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DOI :
10.3791/4091-v
August 13th, 2012
Chapters
0:05
Title
1:36
Expression and Purification of Recombinant Tagged Fusion Proteins in Bacteria
5:21
Cell Culture and Cell Lysate Preparation
6:03
In vitro Assembly of a CFTR-containing Macromolecular Complex (CFTR-PDZK1-MRP4)
8:23
Representative Results
9:06
Conclusion
囊性纤维化跨膜电导调节器(CFTR),上皮细胞的氯离子通道,已与多种蛋白质相互作用和调节重要的细胞过程的CFTR序序介导的相互作用,其中已经有据可查。这个协议描述我们开发组装的PDZ-依赖的CFTR大分子信号复合物的方法在体外。
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