Name: c. elegans tracking and behavioral measurement
Uploaded: 2012-11-17T14:00:00
Description: Watch this Scientific Journal Video about C. elegans Tracking and Behavioral Measurement at JoVE.com

1。跟踪显微镜的说明<ol><li>纤维光源照亮的琼脂板和具有摄像机成像。被安装到该系统的X，Y翻译阶段。 </li><li>的阶段被移动由步进电机的标准，它被连接到一个步进电机控制器。 </li><li>该控制器和相机连接到计算机，并且由自定义程序LabVIEW中编写的控制。 </li><li>摄像机图像的琼脂板的表面，并确定在浅色背景上的暗的物体。 </li><li>的图像质量被调整，使得计算机程序可以量化对象实时。的...

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非侵入性的运动和自然行为的研究需要跟踪技术的合作伙伴，数据压缩技术。在这里，我们已经演示了一个易于使用的跟踪系统，记录详细的图像C.线虫的行为，因为它抓取的琼脂平板的表面上的。在这些图像中包含的信息量是巨大的，高维的，所以我们也开发的方法进行降维的数据导入到只有四个基本措施。这些措施是全面的，是很容易解释的蠕虫行为。对于这项工作，我们向前和向后?...

<table border="1"><tbody><tr><td>的部分的名称</td><td>公司</td><td>目录编号</td><td>评论（可选） </td></tr><tr><td> CCD相机</td><td>巴斯勒</td><td> A601f </td><td></td></tr><tr><td>镜片</td><td>爱特蒙特光学</td><td> MMS系列</td><td></td></tr><tr><td>光纤照明</td><td>杜兰·詹纳</td><td> DC-950H </td><td></td></tr><tr><td>翻译阶段</td><td>大舆</td><td> LS3-4 </td><td></td></tr><tr><td>步进电机</td><td>美国数字</td><td> MS23C </td><td></td></tr><tr><td>步进电机驱动器</td><td>壁虎</td><td> G201 </td><td></td></tr><tr><td>步进电机控制</td><td> SimpleStep </td><td> SSXYZ </td><td></td></tr><tr><td></td><td></td><td></td><td>所有的程序代码是可用的。请发送一个请求到相应的作者的电子邮件。 </td></tr></tbody></table>

c. elegans tracking and behavioral measurement

我们已经开发出了仪表，图像处理和数据分析技术量化的运动器官行为C.线虫 ，因为它的琼脂平板的表面上爬行。为了进行这项研究的遗传，生化和神经行为的基础上，C.线虫是一种理想的生物，因为它是转基因温顺，服从显微镜的，并显示了一些复杂的行为，包括的士，学习，社会交往1,2。行为分析基于跟踪的蠕虫的变动，因为它们的琼脂平皿上抓取的感官行为3，运动4，和一般的突变表型5的研究中是特别有用的。我们的系统的工作原理，通过移动摄像机和照明系统作为蠕虫抓取在一个固定的琼脂平板上，以确保没有机械的刺激传递到蜗杆。我们的跟踪系统是容易使用的，并包括一个半自动的校准功能。阿查llenge的所有视频跟踪系统，它会产生一个巨大的数据量，在本质上是高维。我们的图像处理和数据分析程序，应对这一挑战，减少蠕虫形状为一组独立的组件，全面重建的蠕虫行为的函数只有3-4尺寸6,7。的过程中作为一个例子，我们表明，蠕虫在一个阶段的特定的方式进入和离开它的反转状态。

例如：觅食时，C.线虫转换从正向，逆向运动，经常进行重新定位（欧米茄反过来），然后返回向前运动状态。 ，量化这种转变是很重要的，理解的觅食的运动模式，也该蠕虫的电机控制。使用我们的追踪装置的运动行为，揭示微妙的细节可以看出。 作为一个例子，我们在前进，以扭转和扭转转发〜30分钟的琼脂平板上自由爬行的蠕虫捕捉高清晰度的图像过渡。运行。...

Watch this Scientific Journal Video about C. elegans Tracking and Behavioral Measurement at JoVE.com

C. elegans Tracking and Behavioral Measurement

我们已经开发出一种视频率跟踪显微镜系统，可以记录和量化<em&gt; C.线虫</em&gt;在高分辨率和高转速的行为。我们还开发了计算方法，以减少维数的的蠕虫图像，一组基本的测量，完全描述形状的蠕虫病毒。

线虫跟踪和行为测量

We have developed instrumentation, image processing, and data analysis techniques to quantify the locomotory behavior of C. elegans as it crawls on the ...

Research

JoVE Journal

Neuroscience

C. elegans Tracking and Behavioral Measurement

Assaying &beta;-amyloid Toxicity using a Transgenic C. elegans Model

Assaying ÃƒÅ½Ã‚Â²-amyloid Toxicity using a Transgenic C. elegans Model

含量测定β-淀粉样蛋白的毒性，使用转基因 C。线虫模型

Accumulation of the &beta;-amyloid peptide (A&beta;) is generally believed to be central to the induction of Alzheimer's disease, but the relevant ...

科研

神经科学

C. elegans Positive Butanone Learning, Short-term, and Long-term Associative Memory Assays

C. elegans Positive Butanone Learning, Short-term, and Long-term Associative Memory Assays

线虫的积极丁酮学习，短期，长期联想记忆检测

The memory of experiences and learned information is critical for organisms to make choices that aid their survival. C. elegans navigates its environment ...

In vivo Laser Axotomy in C. elegans

In vivo Laser Axotomy in C. elegans

在 C 在体内激光干切断线虫

Neurons communicate with other cells via axons and dendrites, slender membrane extensions that contain pre- or post-synaptic specializations. If a neuron ...

Automated Quantification of Synaptic Fluorescence in C. elegans

Automated Quantification of Synaptic Fluorescence in C. elegans

突触荧光自动定量<em&gt; C。线虫</em

Synapse strength refers to the amplitude of postsynaptic responses to presynaptic neurotransmitter release events, and has a major impact on overall ...

A Molecular Readout of Long-term Olfactory Adaptation in C. elegans

A Molecular Readout of Long-term Olfactory Adaptation in C. elegans

长期的嗅觉适应性的分子读数<em&gt; C.线虫</em

During sustained stimulation most sensory neurons will adapt their response by decreasing their sensitivity to the signal. The adaptation response helps ...

Hippocampal Insulin Microinjection and In vivo Microdialysis During Spatial Memory Testing

Hippocampal Insulin Microinjection and In vivo Microdialysis During Spatial Memory Testing

海马胰岛素显微注射和<em在体内</em&gt;微透析过程中空间记忆测试

Glucose metabolism is a useful marker for local neural activity, forming the basis of methods such as 2-deoxyglucose and functional magnetic resonance ...

A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo

A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo

单个锥体神经元的高保真光遗传学控制方法<em&gt;在体内</em

Optogenetic methods  have emerged as a powerful tool for elucidating neural circuit activity  underlying a diverse set of behaviors across a broad range ...

In vivo Neuronal Calcium Imaging in C. elegans

In vivo Neuronal Calcium Imaging in C. elegans

C.在体内神经元钙成像技术线虫

The nematode worm C. elegans is an ideal model organism  for relatively simple, low cost neuronal imaging in vivo. Its small transparent body and simple, ...

Long-term Behavioral Tracking of Freely Swimming Weakly Electric Fish

自由游泳弱电鱼的长期行为跟踪

Long-term behavioral tracking can capture and quantify natural animal behaviors, including those occurring infrequently. Behaviors such as exploration and ...

Visualizing Neuroblast Cytokinesis During C. elegans Embryogenesis

Visualizing Neuroblast Cytokinesis During C. elegans Embryogenesis

可视化的神经细胞胞质分裂过程中<em&gt; C。线虫</em&gt;胚胎

This protocol describes the use of fluorescence microscopy to image dividing cells within developing Caenorhabditis elegans embryos. In particular, this ...