Name: modeling colitis-associated cancer with azoxymethane (aom) and dextran sulfate sodium (dss)
Uploaded: 2012-09-11T13:00:00
Description: Watch this Scientific Journal Video about Modeling Colitis-Associated Cancer with Azoxymethane AOM and Dextran Sulfate Sodium DSS at JoVE.com

这项工作是由部分DK089016和L30 RR030244（MAC），CA153036（AS），P30-DK52574（华盛顿大学消化疾病研究核心）。 AIT是霍华德·休斯医学研究所的医学研究培训研究员。

1。溃疡性结肠炎相关性诱发癌症<ol><li>抛开笼子里的性别和年龄相匹配的6-8周龄小鼠用于实验组和对照组。与尾标记或耳夹的小鼠可以单独标记。 </li><li>在第0天，记录基线的权重，并与10毫克/公斤的保持在-20摄氏度AOM的工作溶液（1 mg / ml的等渗盐水中，10毫克/毫升原液蒸馏水稀释，注入每只小鼠腹膜内（IP）） 。经验的基础上，可以调整剂量7-14 mg / kg和/之间，在实验的早期或重复。 </li></ol><p...

<ol>
	<li>Ekbom, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ulcerative+colitis+and+colorectal+cancer.+A+population-based+study.">Ulcerative colitis and colorectal cancer. A population-based study.</a> N. Engl. J. Med. 323, 1228-1233 (1990).</li><li>Terzic, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Inflammation+and+colon+cancer.">Inflammation and colon cancer.</a> Gastroenterology. 138, 2101-2114 (2010).</li><li>Ullman, T. A., Itzkowitz, S. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intestinal+inflammation+and+cancer.">Intestinal inflammation and cancer.</a> Gastroenterology. 140, 1807-1816 (2011).</li><li>Okayasu, I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dysplasia+and+carcinoma+development+in+a+repeated+dextran+sulfate+sodium-induced+colitis+model.">Dysplasia and carcinoma development in a repeated dextran sulfate sodium-induced colitis model.</a> J. Gastroenterol. Hepatol. 17, 1078-1083 (2002).</li><li>Kanneganti, M., Mino-Kenudson, M., Mizoguchi, E. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Animal+models+of+colitis-associated+carcinogenesis.">Animal models of colitis-associated carcinogenesis.</a> J. Biomed. Biotechnol. 342637, (2011).</li><li>Neufert, C., Becker, C., Neurath, M. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+inducible+mouse+model+of+colon+carcinogenesis+for+the+analysis+of+sporadic+and+inflammation-driven+tumor+progression.">An inducible mouse model of colon carcinogenesis for the analysis of sporadic and inflammation-driven tumor progression.</a> Nat. Protoc. 2, 1998-2004 (2007).</li><li>Tanaka, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+novel+inflammation-related+mouse+colon+carcinogenesis+model+induced+by+azoxymethane+and+dextran+sodium+sulfate.">A novel inflammation-related mouse colon carcinogenesis model induced by azoxymethane and dextran sodium sulfate.</a> Cancer Sci. 94, 965-973 (2003).</li><li>De Robertis, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+AOM/DSS+murine+model+for+the+study+of+colon+carcinogenesis:+From+pathways+to+diagnosis+and+therapy+studies.">The AOM/DSS murine model for the study of colon carcinogenesis: From pathways to diagnosis and therapy studies.</a> J. Carcinog. 10, 9 (2011).</li><li>Wirtz, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Chemically+induced+mouse+models+of+intestinal+inflammation.">Chemically induced mouse models of intestinal inflammation.</a> Nat. Protoc. 2, 541-546 (2007).</li><li>Cooper, H. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Clinicopathologic+study+of+dextran+sulfate+sodium+experimental+murine+colitis.">Clinicopathologic study of dextran sulfate sodium experimental murine colitis.</a> Lab Invest. 69, 238-249 (1993).</li><li>Becker, C., Fantini, M. C., Neurath, M. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=High+resolution+colonoscopy+in+live+mice.">High resolution colonoscopy in live mice.</a> Nat. Protoc. 1, 2900-2904 (2006).</li><li>Becker, C., Fantini, M. C., Wirtz, S., Nikolaev, A., Kiesslich, R., Lehr, H. A., Galle, P. R., Neurath, M. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=In+vivo+imaging+of+colitis+and+colon+cancer+development+in+mice+using+high+resolution+chromoendoscopy.">In vivo imaging of colitis and colon cancer development in mice using high resolution chromoendoscopy.</a> Gut. 54, 950-954 (2005).</li><li>Shaker, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Epimorphin+deletion+protects+mice+from+inflammation-induced+colon+carcinogenesis+and+alters+stem+cell+niche+myofibroblast+secretion.">Epimorphin deletion protects mice from inflammation-induced colon carcinogenesis and alters stem cell niche myofibroblast secretion.</a> J. Clin. Invest. 120, 2081-2093 (2010).</li><li>Boivin, G. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Pathology+of+mouse+models+of+intestinal+cancer:+consensus+report+and+recommendations.">Pathology of mouse models of intestinal cancer: consensus report and recommendations.</a> Gastroenterology. 124, 762-777 (2003).</li><li>Cooper, H. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dysplasia+and+cancer+in+the+dextran+sulfate+sodium+mouse+colitis+model.+Relevance+to+colitis-associated+neoplasia+in+the+human:+a+study+of+histopathology,+B-catenin+and+p53+expression+and+the+role+of+inflammation.">Dysplasia and cancer in the dextran sulfate sodium mouse colitis model. Relevance to colitis-associated neoplasia in the human: a study of histopathology, B-catenin and p53 expression and the role of inflammation.</a> Carcinogenesis. 21, 757-768 (2000).</li><li>Yoshida, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+forkhead+box+M1+transcription+factor+contributes+to+the+development+and+growth+of+mouse+colorectal+cancer.">The forkhead box M1 transcription factor contributes to the development and growth of mouse colorectal cancer.</a> Gastroenterology. 132, 1420-1431 (2007).</li><li>Suzuki, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Strain+differences+in+the+susceptibility+to+azoxymethane+and+dextran+sodium+sulfate-induced+colon+carcinogenesis+in+mice.">Strain differences in the susceptibility to azoxymethane and dextran sodium sulfate-induced colon carcinogenesis in mice.</a> Carcinogenesis. 27, 162-169 (2006).</li><li>Mahler, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Differential+susceptibility+of+inbred+mouse+strains+to+dextran+sulfate+sodium-induced+colitis.">Differential susceptibility of inbred mouse strains to dextran sulfate sodium-induced colitis.</a> Am. J. Physiol. 274, 544-551 (1998).</li><li>Nambiar, P. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Preliminary+analysis+of+azoxymethane+induced+colon+tumors+in+inbred+mice+commonly+used+as+transgenic/knockout+progenitors.">Preliminary analysis of azoxymethane induced colon tumors in inbred mice commonly used as transgenic/knockout progenitors.</a> Int. J. Oncol. 22, 145-150 (2003).</li><li>Tanaka, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Colorectal+carcinogenesis:+Review+of+human+and+experimental+animal+studies.">Colorectal carcinogenesis: Review of human and experimental animal studies.</a> J Carcinog. 8, (2009).</li><li>Ciorba, M. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Induction+of+IDO-1+by+immunostimulatory+DNA+limits+severity+of+experimental+colitis.">Induction of IDO-1 by immunostimulatory DNA limits severity of experimental colitis.</a> J. Immunol. 184, 3907-3916 (2010).</li><li>Kerr, T. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Dextran+sodium+sulfate+inhibition+of+real-time+polymerase+chain+reaction+amplification:+A+poly-A+purification+solution.">Dextran sodium sulfate inhibition of real-time polymerase chain reaction amplification: A poly-A purification solution.</a> Inflamm. Bowel Dis. 18, 344-348 (2012).</li><li>Tang, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=is+required+for+resection-induced+changes+in+apoptosis,+proliferation,+and+members+of+the+extrinsic+cell+death+pathways.">is required for resection-induced changes in apoptosis, proliferation, and members of the extrinsic cell death pathways.</a> Gastroenterology. 126, 220-230 (2004).</li></ol>

AOM和DSS的小鼠迅速和有效的模型人结肠炎相关的癌症的治疗。假设关于遗传因素结肠炎相关癌症可以容易地研究与遗传工程小鼠结肠炎相关癌症的效果的药理学目标13，16或者，可以通过采用野生型小鼠研究。 虽然这种模式是高度重视的那些有兴趣在结肠肿瘤的发生发展中的设置炎症的研究存在局限性。在这个模型中小鼠中观察到疾病的程度可以有很大的不同，这取?...

<table border="1"><tbody><tr><td> 的试剂的名称 </td><td> 公司 </td><td> 目录编号 </td><td> 评论 </td></tr><tr><td> C57BL/6J小鼠</td><td>杰克逊实验室</td><td> 000664 </td><td></td></tr><tr><td>氧化偶氮甲烷（AOM） </td><td> Sigma Aldrich公司</td><td> A5486-100MG </td><td> 储备溶液：稀释至10被保持在-20℃下在蒸馏水中为0.5毫克/毫升- 1毫升的等分试样。 解决方案：1 mg / ml的等渗（0.9％）盐水稀释股票</td></tr><tr><td>葡聚糖硫酸酯钠（DSS） </td><td> TDB咨询</td><td> DB001 </td><td> MW 40 kDa的（36-50 kDa的准备从其他来源是可接受的;应该使用相同的特定的一个单一的实验）6 </td></tr><tr><td> Coloviewminiendoscopic系统</td><td>卡尔斯托斯</td><td>多种</td><td> Becker 等人 11的设备和设置的详细的解释。 </td></tr><tr><td> TPP快速FILTERMAX 500毫升瓶，过滤器，0.22微米PES </td><td>中西部地区科学</td><td> TP99500 </td><td>任何标准组织文化过滤器是可以接受的</td></tr><tr><td>乙醇200证明ASC / USP </td><td>药物AAPER（或其它） </td><td> 11ACS200 </td><td>在蒸馏水中，稀释至70％的</td></tr><tr><td>异氟醚，USP </td><td>巴特勒动物保健供应</td><td> 4029405 </td><td>将鼠标玻璃瓶中，用纱布或小布浸泡在麻醉</td></tr><tr><td> 18G直灌胃针</td><td>布伦特里科学</td><td> N-008 </td><td></td></tr><tr><td>磷酸盐缓冲液（PBS） </td><td>西格玛Aldrich公司</td><td> P5493 </td><td>在蒸馏水中稀释至1X（0.01M） </td></tr><tr><td>冷托盘（II冷板组织康） </td><td> Fisher Scientific则</td><td> NC9491941 </td><td>存放在-20°C </td></tr><tr><td> ImageJ软件</td><td>美国国立卫生研究院（免费下载） </td><td></td><td> <a href="http://rsbweb.nih.gov/ij/" target="_blank">http://rsbweb.nih.gov/ij/</a> </td></tr><tr><td>甲醛（37％） </td><td> Fisher Scientific则</td><td> F79-500 </td><td>在PBS中稀释至10％的</td></tr><tr><td> BD Bacto琼脂</td><td> Fisher Scientific则</td><td> DF0140-01-0 </td><td>使用烤盘创建2％的溶液，在蒸馏水中</td></tr><tr><td> MILTEX眼睛敷料钳</td><td> MedPlus公司</td><td> 18-780 </td><td></td></tr><tr><td> MILTEX眼睛剪刀</td><td> MedPlus公司</td><td> 18-1430 </td> &lt;TD“&gt;”，曲线点期间防止损坏结肠开幕。 </td></tr><tr><td>经Alcian Blue 8GX（粉末） </td><td> Sigma Aldrich公司</td><td> A5268 </td><td> 1克粉末添加到100毫升3％醋酸（3毫升冰醋酸+ 97毫升蒸馏水中） </td></tr><tr><td> 1毫升结核菌素皮锥针注射器附有26克×3/8 </td><td> BD </td><td> 305946 </td><td>对于注射AOM </td></tr></tbody></table>

modeling colitis-associated cancer with azoxymethane (aom) and dextran sulfate sodium (dss)

个人与炎症性肠病（IBD），如克罗恩病（CD）和溃疡性结肠炎（UC）是在健康的个人发展结直肠癌（CRC）的风险增加。这种风险是成正比的持续时间和程度的疾病，结肠发育不良鸡传染性法氏囊病和结肠炎相关癌症（CAC）被认为是发展的结果，累计发病率高达30％，个人长期的UC结肠的广泛参与。上皮细胞的损伤和修复的重复循环，而这些细胞沐浴在一种慢性炎症性细胞因子环境2虽然自发和结肠炎相关的癌症的共享的质量是腺癌，被认为是相关的分子事件的序列是不同的。3这区分认为需要特定动物模型的CAC。 目前存在的一些小鼠模型的研究CAC。硫酸葡聚糖SODIUM（DSS），代理人对结肠上皮细胞的直接毒性作用，可管理的饮用水中创建多个周期的小鼠慢性炎症状态。有足够的时间，这些小鼠肿瘤的发展。4肿瘤的发展，赶紧在这个模型中，如果一个亲致癌设置的管理。这些包括与遗传突变小鼠在肿瘤发生途径（APC，p53基因，MSH2），以及预先处理的基因毒性剂（氧化偶氮甲烷[AOM]，1,2 -二甲基肼[DMH]）。5小鼠与AOM相结合的DSS结肠炎相关癌症的典范，重现性，效力，低廉的价格，和易用性已经得到普及。虽然他们有一个共享的机制，AOM已被发现是更有效和稳定的溶液中比DMH。虽然肿瘤的发生发展中的其他车型，一般需要几个月，注射AOM，随后用DSS小鼠肿瘤的制定适当的的小为7-10周。6，7的最后，AOM和DSS可以施用任何遗传背景的小鼠（基因敲除，转基因等），而不到一个特定的致瘤菌株杂交育种。在这里，我们展示了一个协议驱动的炎症结肠肿瘤在小鼠体内利用一个单一的注射AOM三个7天为一个周期超过10周的时间DSS。这种模式导致肿瘤的组织学和分子的变化密切类似于发生在人类CAC，在本病的发生和化学预防的研究提供了一个非常有价值的模型8

Watch this Scientific Journal Video about Modeling Colitis-Associated Cancer with Azoxymethane AOM and Dextran Sulfate Sodium DSS at JoVE.com

Modeling Colitis-Associated Cancer with Azoxymethane AOM and Dextran Sulfate Sodium DSS

我们展示了一个协议，氧化偶氮甲烷（AOM）的遗传毒性剂，其中给药后由促炎性剂硫酸葡聚糖钠（DSS）的迅速的三个周期的和一贯生成结肠肿瘤在小鼠与在人类结肠炎所见的形态学和分子的相似之处相关的癌症。

建模结肠炎相关的癌症与​​氧化偶氮甲烷（AOM）和葡聚糖硫酸钠（DSS）

Individuals with inflammatory bowel disease (IBD), such as Crohn's disease (CD) or ulcerative colitis (UC) are at increased risk of developing colorectal ...

Research

JoVE Journal

Medicine

Modeling Colitis-Associated Cancer with Azoxymethane (AOM) and Dextran Sulfate Sodium (DSS)

Modeling Stroke in Mice - Middle Cerebral Artery Occlusion with the Filament Model

建模在小鼠体内的行程 - 中东与灯丝模型大脑中动脉闭塞

Stroke is among the most frequent causes of death and adult disability, especially in highly developed countries. However, treatment options to date are ...

科研

医学

Modeling and Imaging 3-Dimensional Collective Cell Invasion

3维建模和成像集体细胞的侵袭

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Heterotypic Three-dimensional In Vitro Modeling of Stromal-Epithelial Interactions During Ovarian Cancer Initiation and Progression

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Retinal Detachment Model in Rodents by Subretinal Injection of Sodium Hyaluronate

视网膜脱离模型鼠害由透明质酸钠的视网膜下注射

Subretinal injection of sodium hyaluronate is a widely accepted method of inducing retinal detachment (RD). However, the height and duration of RD or the ...

Modeling Spontaneous Metastatic Renal Cell Carcinoma (mRCC) in Mice Following Nephrectomy

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在小鼠模型自发转移性肾细胞癌（MRCC）肾切除

One of the key challenges to improved testing of new experimental therapeutics in renal cell carcinoma (RCC) is the development of models that faithfully ...

Working with Human Tissues for Translational Cancer Research

与人体组织转化为癌症研究工作

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Encephalopathy of prematurity (EoP) is a term that encompasses the central nervous system (CNS) abnormalities associated with preterm birth. To best ...

Modeling the Early Steps of Ovarian Cancer Dissemination in an Organotypic Culture of the Human Peritoneal Cavity

造型卵巢癌传播在人类腹腔的器官文化的早期步骤

The pattern of ovarian cancer metastasis is markedly different from that of most other epithelial tumors, because it rarely spreads hematogenously. ...

Modeling Chemotherapy Resistant Leukemia In Vitro

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建模化疗耐药白血病<em&gt;体外</em

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There are many advantages of direct nose-to-brain drug delivery in the treatment of neurological disorders. However, its application is limited by the ...

建模结肠炎相关的癌症与​​氧化偶氮甲烷（AOM）和葡聚糖硫酸钠（DSS）

建模结肠炎相关的癌症与氧化偶氮甲烷（AOM）和葡聚糖硫酸钠（DSS）