Name: orthotopic small bowel transplantation in rats
Uploaded: 2012-11-06T13:00:00
Description: Watch this Scientific Journal Video about Orthotopic Small Bowel Transplantation in Rats at JoVE.com

1。供体手术<ol><li>应该保持供体鼠禁食24小时（水/葡萄糖溶液的免费访问）。 </li><li> ，引起吸入异氟醚麻醉，开始以2％的标准雾化器，然后降低到1％后进行剖腹手术。执行脚趾捏了把检查镇静。 </li><li>刮腹部和清洁皮肤准备3次（Kodan）。然后执行后正中切口皮下给药的镇痛。 </li><li>后移植物包裹在生理盐水浸泡过的纱布，分离胰腺和升结肠，轻轻地用一个Q-尖端（6倍放大倍率的显微镜下的?...

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	<li>Abu-Elmagd, K. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Five+Hundred+Intestinal+and+Multivisceral+Transplantations+at+a+Single+Center:+Major+Advances+With+New+Challenges.">Five Hundred Intestinal and Multivisceral Transplantations at a Single Center: Major Advances With New Challenges.</a> Ann. Surg. 250 (4), 567-56 (2009).</li><li>Berney, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Portal+versus+systemic+drainage+of+small+bowel+allografts:+comparative+assessment+of+survival,+function,+rejection,+and+bacterial+translocation.">Portal versus systemic drainage of small bowel allografts: comparative assessment of survival, function, rejection, and bacterial translocation.</a> J. Am. Coll. Surg. 195 (6), 804 (2002).</li><li>Fishbein, T. 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J. Transplant. 10 (11), 2431 (2010).</li><li>Schaefer, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Resident+macrophages+are+involved+in+intestinal+transplantation-associated+inflammation+and+motoric+dysfunction+of+the+graft+muscularis.">Resident macrophages are involved in intestinal transplantation-associated inflammation and motoric dysfunction of the graft muscularis.</a> Am J Transplant. 7 (5), 1062 (2007).</li><li>Schaefer, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mechanism+and+impact+of+organ+harvesting+and+ischemia-reperfusion+injury+within+the+graft+muscularis+in+rat+small+bowel+transplantation.+Transplant.">Mechanism and impact of organ harvesting and ischemia-reperfusion injury within the graft muscularis in rat small bowel transplantation. 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最近采用的模型大鼠小肠移植模型中被描述为早在1970ies 5涉及异位小肠移植使用不同的技术13。异位一般程序的优点是更容易的显微外科技术，更容易获得移植物的评估，异位小肠移植有很大的缺点，不考虑的移植小肠的收缩活动功能等方面的多重相互作用黏膜屏障功能提供了广阔的主机的共生和病原体的背景下，原位移植的特点。我们的团队已获得了大量的经验，本文描述的的?...

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orthotopic small bowel transplantation in rats

小肠移植已成为公认的短肠综合征和失败的肠外营养（不可逆肠衰竭）患者的临床治疗选择。在专门的医疗中心，提高操作和管理策略导致了优异的短期和中期患者和移植物存活率，同时提供高品质的生活1,3。不同的是较常见的其他实体器官移植（ 即心脏，肝脏）许多潜在的诱导小肠移植的移植肾功能和免疫学改变的机制尚不完全清楚6,7。发作的急性排斥反应，败血症，慢性移植物功能衰竭的主要障碍仍然较为有利的长期结果，并阻碍更广泛的就业的过程中，尽管越来越多的家庭肠外营养的患者可能受益于这样的移植。小肠包含乘客白细胞的大量的，通常被称为作为肠相关的淋巴系统（GALT）肠道接枝的高免疫原性的原因，这是一部分的一部分。许多共生和病原体在肠道中的存在和靠近解释脓毒症发作的严重程度，一旦接枝粘膜的完整性受到损害（例如，通过抑制）。为了推动该领域的肠和多器官移植产生的数据更可靠和可行的动物模型是必要的。本文结合该模型的可靠性和可行性一旦确立以标准化的方式，可以在底层的复杂的分子，细胞和功能的机制所触发的小肠移植提供了宝贵的见解。我们已经成功地使用超过5年以上的在我们的实验室8-11细化所描述的过程。朱庇特的视频格式是特别有用的，表现出复杂的多项式dure，避免最初的规划，建立原位啮齿动物​​模型研究小肠移植组的陷阱。

术后正常移植的动物迅速恢复的过程中，下加热灯约1小时。低温的术后死亡率的一个重要原因，应谨慎地避免内和术后。皮下注射2.5毫升生理盐水加2.5毫升的5％葡萄糖每8个小时，第24小时，术中的流体损失，必须更换。大鼠也应该有术后第一个24小时免费使用葡萄糖溶液（或凝胶饮食）和水区。在此期间，他们应该免费使用标准大鼠饲料和水自由采食恢复​​正常进食行为。?...

Watch this Scientific Journal Video about Orthotopic Small Bowel Transplantation in Rats at JoVE.com

Orthotopic Small Bowel Transplantation in Rats

小肠移植已成为一种公认的不可逆肠衰竭患者的治疗选择。我们作为一个可靠的工具，以解决潜在的免疫和炎症过程的复杂小肠移植大鼠原位小肠移植的实验模型。

大鼠原位小肠移植

Small  bowel transplantation has become an accepted clinical option for patients with  short gut syndrome and failure of parenteral nutrition ...

Research

JoVE Journal

Medicine

Development of Obliterative Bronchiolitis in a Murine Model of Orthotopic Lung Transplantation

闭塞性细支气管炎在原位肺移植小鼠模型的发展

Orthotopic lung transplantation in rats was first reported by Asimacopoulos and colleagues in 1971 1. Currently, this method is well accepted and ...

科研

医学

Orthotopic Liver Transplantation in Rats

大鼠原位肝移植

Clinical progress in the field of liver transplantation has been largely supported by animal models1,2. Since the publication of the first orthotopic rat ...

Orthotopic Aortic Transplantation in Mice for the Study of Vascular Disease

原位主动脉瓣移植在小鼠血管疾病的研究

Vascular procedures involving anastomoses in the mouse are generally thought to be difficult and highly dependent on the skill of the individual surgeon. ...

Orthotopic Hind Limb Transplantation in the Mouse

原位后肢移植中的鼠标

In vivo animal model systems, and in particular mouse models, have evolved into powerful and versatile scientific tools indispensable to basic and ...

Transarterial Administration of Oncolytic Viruses for Locoregional Therapy of Orthotopic HCC in Rats

溶瘤病毒的Transarterial管理局大鼠原位肝癌的局部治疗

Hepatocellular carcinoma (HCC) is a disease with limited treatment options and poor prognosis.  In recent years, oncolytic virotherapies have proven ...

A Pre-Clinical Porcine Model of Orthotopic Heart Transplantation

正交心脏移植的临床前猪模型

Fifty-years following the first successful report, cardiac transplantation remains the gold-standard treatment for eligible patients with advanced heart ...

Orthotopic Rat Kidney Transplantation: A Novel and Simplified Surgical Approach

大鼠原位肾移植: 一种新的简化的手术方法

Kidney transplantation offers increased survival rates and improved quality of life for patients with end-stage renal disease, as compared to any type of ...

Reduced Complications after Arterial Reconnection in a Rat Model of Orthotopic Liver Transplantation

矫形肝移植大鼠模型中动脉重联后并发症减少

The rat orthotopic liver transplantation (OLT) model is a powerful tool to study acute and chronic rejection. However, it is not a complete representation ...

Left Lung Orthotopic Transplantation in a Juvenile Porcine Model for ESLP

ESLP幼猪模型中的左肺原位移植

Lung transplantation is the gold-standard treatment for end-stage lung disease, with over 4,600 lung transplantations performed worldwide annually. ...

A Rat Orthotopic Renal Transplantation Model for Renal Allograft Rejection

一种用于肾同种异体排斥的大鼠原位肾移植模型

Renal allograft rejection limits the long-term survival of patients after renal transplantation. Rat orthotopic renal transplantation is an essential ...