Name: orthotopic small bowel transplantation in rats
Uploaded: 2012-11-06T13:00:00
Description: Watch this Scientific Journal Video about Orthotopic Small Bowel Transplantation in Rats at JoVE.com

1. Donor Operation <ol><li> Donoren rotter holdes fastende i 24 timer (fri adgang til vand / glucoseopløsning). </li><li> For at inducere Isofluran inhalation anæstesi, skal du starte med 2% på standard atomizer, og derefter reducere til 1% efter udførelse laparotomi. Udfør en tå knibe for at kontrollere sedation. </li><li> Barbere maven og rengør med hud prep 3 gange (Kodan). Derefter udføre en median incision efter subkutan administration af det smertestillende stof. </li><li> Efter at implantatet er pakket ...

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	<li>Abu-Elmagd, K. M., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Five+Hundred+Intestinal+and+Multivisceral+Transplantations+at+a+Single+Center:+Major+Advances+With+New+Challenges.">Five Hundred Intestinal and Multivisceral Transplantations at a Single Center: Major Advances With New Challenges.</a> Ann. Surg. 250 (4), 567-56 (2009).</li><li>Berney, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Portal+versus+systemic+drainage+of+small+bowel+allografts:+comparative+assessment+of+survival,+function,+rejection,+and+bacterial+translocation.">Portal versus systemic drainage of small bowel allografts: comparative assessment of survival, function, rejection, and bacterial translocation.</a> J. Am. Coll. Surg. 195 (6), 804 (2002).</li><li>Fishbein, T. 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S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transplantation+of+small+bowel+in+the+rat:+technical+and+immunological+considerations.">Transplantation of small bowel in the rat: technical and immunological considerations.</a> Surgery. 70 (5), 693 (1971).</li><li>Murase, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Graft-versus-host+disease+after+brown+Norway-to-Lewis+and+Lewis-to-Brown+Norway+rat+intestinal+transplantation+under+FK506.">Graft-versus-host disease after brown Norway-to-Lewis and Lewis-to-Brown Norway rat intestinal transplantation under FK506.</a> Transplantation. 55 (1), 1 (1993).</li><li>Murase, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Immunomodulation+of+intestinal+transplant+with+allograft+irradiation+and+simultaneous+donor+bone+marrow+infusion.">Immunomodulation of intestinal transplant with allograft irradiation and simultaneous donor bone marrow infusion.</a> Transplant Proc. 31 (1-2), 565 (1999).</li><li>Pech, T., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Perioperative+infliximab+application+ameliorates+acute+rejection+associated+inflammation+after+intestinal+transplantation.">Perioperative infliximab application ameliorates acute rejection associated inflammation after intestinal transplantation.</a> Am. J. Transplant. 10 (11), 2431 (2010).</li><li>Schaefer, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Resident+macrophages+are+involved+in+intestinal+transplantation-associated+inflammation+and+motoric+dysfunction+of+the+graft+muscularis.">Resident macrophages are involved in intestinal transplantation-associated inflammation and motoric dysfunction of the graft muscularis.</a> Am J Transplant. 7 (5), 1062 (2007).</li><li>Schaefer, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mechanism+and+impact+of+organ+harvesting+and+ischemia-reperfusion+injury+within+the+graft+muscularis+in+rat+small+bowel+transplantation.+Transplant.">Mechanism and impact of organ harvesting and ischemia-reperfusion injury within the graft muscularis in rat small bowel transplantation. Transplant.</a> Proc. 38 (6), 1821 (2006).</li><li>Schaefer, N., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Acute+rejection+and+the+muscularis+propria+after+intestinal+transplantation:+the+alloresponse,+inflammation,+and+smooth+muscle+function.">Acute rejection and the muscularis propria after intestinal transplantation: the alloresponse, inflammation, and smooth muscle function.</a> Transplantation. 85 (10), 1465 (2008).</li><li>Xue, L., et al. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Surgical+experience+of+refined+3-cuff+technique+for+orthotopic+small-bowel+transplantation+in+rat:+a+report+of+270+cases.">Surgical experience of refined 3-cuff technique for orthotopic small-bowel transplantation in rat: a report of 270 cases.</a> Am. J. Surg. 198 (1), 110 (2009).</li><li>Zhang, X. 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Mens intestinaltransplanterede modeller i rotter er blevet beskrevet så tidligt som i 1970&#39;erne 5 mest af de nyligt anvendte modeller involverer heterotop intestinaltransplantation hjælp af forskellige teknikker 13. Mens heterotopiske procedurer generelt har den fordel lettere mikrokirurgiske teknikker og lettere tilgængelighed af transplantatet til bedømmelse, heterotop intestinaltransplantation har den store ulempe, at der ikke tages hensyn til de forskellige vekselvirkninger mellem trans...

<table border="1"><tbody><tr><td> Navn på reagenset </td><td> Firma </td><td> Katalognummer </td><td> Kommentarer (valgfrit) </td></tr><tr><td> University of Wisconsin (UW) opløsning (ViaSpan) </td><td> Bristol-Myers Squibb </td><td></td><td></td></tr><tr><td> Uro-Nebacetin N opløsning </td><td> Nycomed </td><td> 6967855 </td><td></td></tr><tr><td> Ampicillin </td><td> Ratiopharm </td><td></td><td></td></tr><tr><td> Carprofen (Rimadyl) </td><td> Pfizer </td><td></td><td></td></tr><tr><td> Prolene 10-0 unresorbable sutur </td><td> Ethicon </td><td></td><td></td></tr><tr><td> Monocryl 6-0 resorberbar sutur </td><td> Ethicon </td><td></td><td></td></tr><tr><td> Vicryl 3-0 resorberbar sutur </td><td> Ethicon </td><td></td><td></td></tr><tr><td> iv Kateter G 20 1.1x33 mm </td><td> Braun </td><td></td><td></td></tr><tr><td> iv Kateter G 22 0.9x25 mm </td><td> Braun </td><td></td><td></td></tr><tr><td> Kodan Skin Prep </td><td> Schülke </td><td></td><td></td></tr><tr><td> NaCl 0,9% Infusionsopløsning </td><td> Braun </td><td></td><td></td></tr><tr><td> Buede tang small </td><td> FineScienceTools </td><td> 11.009-13 </td><td></td></tr><tr><td> Micro pincet buede </td><td> AESCULAP </td><td> BD 333 </td><td></td></tr><tr><td> Micro pincet buede </td><td> AESCULAP </td><td> FD281R </td><td></td></tr><tr><td> Micro pincet lige 1 </td><td> WPI </td><td> 5 </td><td></td></tr><tr><td> Micro pincet lige 2 </td><td> WPI </td><td> 2 </td><td></td></tr><tr><td> Micro nåleholder </td><td> WPI </td> &lt;td&gt; 14.081 </td><td></td></tr><tr><td> Micro saks </td><td> FineScienceTools </td><td> 15.006-09 </td><td></td></tr><tr><td> Micro skalpel </td><td> MANI </td><td> Ophthalmic kniv </td><td></td></tr><tr><td> Micro klemmer </td><td> AESCULAP </td><td> FB329R </td><td></td></tr></tbody></table>

orthotopic small bowel transplantation in rats

Tyndtarmstransplantation er blevet en accepteret klinisk mulighed for patienter med korte tarm syndrom og svigt af parenteral ernæring (irreversibel intestinal fiasko). I specialiserede centre forbedrede operative og forvaltning strategier har ført til fremragende kort-og mellemliggende sigt patient og transplantat overlevelse samtidig med høj livskvalitet 1,3. Til forskel fra mere almindelige transplantation af andre faste organer (dvs. hjerte, lever) mange underliggende mekanismer af transplantatfunktion og immunologiske ændringer induceret af intestinaltransplantation er ikke helt kendt 6,7. Episoder med akut afstødning, sepsis og kronisk graft svigt er de vigtigste hindringer, som stadig bidrager til mindre gunstige langsigtede resultat og hindrede en mere udbredt beskæftigelse af proceduren på trods af et stigende antal patienter på hjemmebane parenteral ernæring, som potentielt ville drage fordel af en sådan transplantation. Tyndtarmen indeholderet stort antal af passager leukocytterne almindeligvis betegnes som en del af tarmen forbundet lymfoide system (GALT) det er en del af årsagen til det høje immunogenity af intestinal graft. Tilstedeværelsen og tætte nærhed af mange kommensale og patogener i tarmen forklarer sværhedsgraden af ​​sepsis episoder når graft mucosal integritet kompromitteres (for eksempel ved afstødning). At fremme inden for intestinale-og multiorgan transplantation flere data genereret fra pålidelige og gennemførlige dyremodeller er nødvendig. Modellen tilvejebragt heri kombinerer både pålidelighed og gennemførlighed når de er etableret på en standardiseret måde og kan give værdifuld indsigt i de underliggende komplekse molekylære, cellulære og funktionelle mekanismer, der er udløst af intestinale transplantationer. Vi har med succes brugt og forfinet den beskrevne procedure i løbet af mere end 5 år i vores laboratorium 8-11. Den Jové video-baseret format er især nyttig til at demonstrere den komplekse procedurersesprocedure og undgå de første faldgruber for grupper planlægger at etablere en ortotopisk gnavermodel undersøge intestinaltransplanterede.

Normal postoperativ kursus De transplanterede dyr bør komme sig hurtigt fra proceduren under en varmelampe i ca 1 time. Hypotermi er en væsentlig årsag til postoperativ dødelighed og bør omhyggeligt undgås intra-og postoperativt. Intraoperative væsketab skal udskiftes ved sc injektion af 2,5 ml normalt saltvand plus 2,5 ml Glucose 5% hver 8. time i de første 24 timer. Rotterne skal også have fri adgang til glucoseopløsning (eller gel kost) og vand po for de første 24 timer postoperat...

Watch this Scientific Journal Video about Orthotopic Small Bowel Transplantation in Rats at JoVE.com

Orthotopic Small Bowel Transplantation in Rats

Tyndtarmstransplantation er blevet en accepteret behandling for patienter med irreversibel intestinal fiasko. Vores eksperimentelle model af orthotopisk tyndtarmstransplantation i rotter tjener som et pålideligt værktøj til at løse den underliggende immunologiske og inflammatoriske processer, der komplicerer intestinaltransplanterede.

Orthotopisk tyndtarmstransplantation i rotter

Small  bowel transplantation has become an accepted clinical option for patients with  short gut syndrome and failure of parenteral nutrition ...

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Medicine

Development of Obliterative Bronchiolitis in a Murine Model of Orthotopic Lung Transplantation

Udvikling af obliterativ bronchiolitis i en musemodel for Orthotopisk lungetransplantation

Orthotopic lung transplantation in rats was first reported by Asimacopoulos and colleagues in 1971 1. Currently, this method is well accepted and ...

Orthotopic Liver Transplantation in Rats

Ortotopisk levertransplantation i rotter

Clinical progress in the field of liver transplantation has been largely supported by animal models1,2. Since the publication of the first orthotopic rat ...

Orthotopic Aortic Transplantation in Mice for the Study of Vascular Disease

Ortotopisk Aorta Transplantation i mus for Studiet af Vascular Disease

Vascular procedures involving anastomoses in the mouse are generally thought to be difficult and highly dependent on the skill of the individual surgeon. ...

Orthotopic Hind Limb Transplantation in the Mouse

Ortotopisk Hind Limb Transplantation i Mus

In vivo animal model systems, and in particular mouse models, have evolved into powerful and versatile scientific tools indispensable to basic and ...

Transarterial Administration of Oncolytic Viruses for Locoregional Therapy of Orthotopic HCC in Rats

Transarterial Administration af onkolytiske virus for Lokoregional Therapy af ortotopisk HCC i rotter

Hepatocellular carcinoma (HCC) is a disease with limited treatment options and poor prognosis.  In recent years, oncolytic virotherapies have proven ...

A Pre-Clinical Porcine Model of Orthotopic Heart Transplantation

En præklinisk Porcin model af Ortotopisk hjerte transplantation

Fifty-years following the first successful report, cardiac transplantation remains the gold-standard treatment for eligible patients with advanced heart ...

Orthotopic Rat Kidney Transplantation: A Novel and Simplified Surgical Approach

Ortotopic rotte nyre transplantation: en roman og forenklet kirurgisk tilgang

Kidney transplantation offers increased survival rates and improved quality of life for patients with end-stage renal disease, as compared to any type of ...

Reduced Complications after Arterial Reconnection in a Rat Model of Orthotopic Liver Transplantation

Reduceret komplikationer efter arteriel gentilslutning i en rotte model af ortopisk levertransplantation

The rat orthotopic liver transplantation (OLT) model is a powerful tool to study acute and chronic rejection. However, it is not a complete representation ...

Left Lung Orthotopic Transplantation in a Juvenile Porcine Model for ESLP

Venstre lunge ortopisk transplantation i en ung svin model for ESLP

Lung transplantation is the gold-standard treatment for end-stage lung disease, with over 4,600 lung transplantations performed worldwide annually. ...

A Rat Orthotopic Renal Transplantation Model for Renal Allograft Rejection

En rotte ortotopisk nyretransplantationsmodel til afvisning af nyreallograft

Renal allograft rejection limits the long-term survival of patients after renal transplantation. Rat orthotopic renal transplantation is an essential ...