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DOI :
10.3791/4140-v
•
8:05 min
June 27th, 2012
Chapters
0:05
Title
1:05
Growing and Harvesting HeLa Cells for Nuclear Extraction
1:56
Swell HeLa Cells in Hypotonic Buffer
3:00
Lyse Cells using a Dounce Homogenizer
4:27
Salt-extract the Nuclei
5:15
Concentrate and Dialyze the Nuclear Extract
6:16
Results: Small-scale Nuclear Extract Technique for Functional Assays of Gene Expression Machineries
7:51
Conclusion
一个健壮的,小规模的HeLa细胞的核提取物制备的协议。该协议是为需要使用小种群的细胞,如细胞治疗药物或RNAi,有价值的实验。该方法应该适用于各种各样的基因表达分析和其他类型的细胞,包括病人的细胞。
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