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DOI :
10.3791/4140-v
•
8:05 min
June 27th, 2012
Chapters
0:05
Title
1:05
Growing and Harvesting HeLa Cells for Nuclear Extraction
1:56
Swell HeLa Cells in Hypotonic Buffer
3:00
Lyse Cells using a Dounce Homogenizer
4:27
Salt-extract the Nuclei
5:15
Concentrate and Dialyze the Nuclear Extract
6:16
Results: Small-scale Nuclear Extract Technique for Functional Assays of Gene Expression Machineries
7:51
Conclusion
堅牢な、小規模のHeLa細胞核抽出物を調製するためのプロトコルが記述されています。このプロトコルはそのような薬剤又はRNAi処理した細胞などの細胞の小集団の使用を必要とするアッセイのために貴重なものです。方法は、患者の細胞を含む遺伝子発現アッセイおよび他の細胞さまざまな種類のに適用する必要があります。
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