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26.3K Views
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09:42 min
August 17th, 2012
DOI :
10.3791/4141-v
Chapters
0:05
Title
1:50
Preparing Neuronal Culture Coverslips
2:37
Collecting Adult Mouse DRG Neurons
4:12
Preparing Adult Mouse DRG Neurons for Electroporation
5:40
Electroporation and Culturing of Neurons
8:03
Genetically Manipulated Adult Mouse DRG Cultures
9:15
Conclusion
Summary
不定冠詞 in vitroでモデルが記載されています。方法は、遺伝子操作を受けているニューロンから軸索の再成長を可能にするre-suspension/re-platingステップを含む。このアプローチは、RNAiベースのタンパク質のノックダウンを使用して、軸索再生の機能喪失研究のために特に便利です。
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