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DOI :
10.3791/4145-v
•
13:48 min
June 24th, 2012
Chapters
0:05
Title
1:34
Procedure Overview
3:16
RNA Transcription and Capping for the Recovery of Infectious MNV
6:27
MNV Recovery by RNA Electroporation into Raw Cells
8:25
Recovery by Lipofection into BSR-T7 Cells
10:07
Direct Recovery of Infectious MNV from cDNA in Cells Expressing T7 RNA Polymerase
12:20
Representative Results
Noroviruses들이 특성화를위한 위장염 아직 분자 기술의 주요 원인은 아직 비교적 새로운입니다. 여기 murine 노로 바이러스 (MNV), 세포 배양에서 증식 수있는이 속의 유일한 구성원의 효율적인 복구를위한 두 개의 역방향 유전학 방법을보고합니다.
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