Name: fabrication and application of rose bengal-chitosan films in laser tissue repair
Uploaded: 2012-10-23T15:00:00
Description: Watch this Scientific Journal Video about Fabrication and Application of Rose Bengal-chitosan Films in Laser Tissue Repair at JoVE.com

支持这项工作是由西悉尼大学研究资助。

1。壳聚糖黏胶剂<ol><li>脱乙酰壳多糖粉末溶解在乙酸溶液中，用于制备乙酸2％（体积/体积）的储备液，添加10毫升冰醋酸的490毫升去离子水（DI-H 2 O）。 </li><li>如果在孟加拉玫瑰红（RB）在乙酸（0.01％重量/体积）的储备液的制备中，称量5毫克的RB的小瓶中，用铝箔包裹，以避免光漂白。 DI-H 2 O中加入0.5 ml溶解的粉末，然后加49.5毫升的醋酸原液（详见第1.1节）。 </li><li>为了制?...

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	<li>O'Neill, A. C., Winograd, J. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microvascular+anastomosis+using+a+photochemical+tissue+bonding+technique.">Microvascular anastomosis using a photochemical tissue bonding technique.</a> Lasers Surg. Med. 39, 716-722 (2007).</li><li>Yao, M., Yaroslavsky, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Phototoxicity+is+not+associated+with+photochemical+tissue+bonding+of+skin.">Phototoxicity is not associated with photochemical tissue bonding of skin.</a> Lasers Surg. 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玫瑰粘合剂制造是基于干铸在一个简单的过程，但在酸性pH条件下，需要延长的RB的溶解的脱乙酰壳多糖溶液中搅拌。这是重要的，让溶液干涸，直到它成为一个水不溶性膜。这种情况发生时的重量的水的含量为〜10％，在干燥的膜6。不溶性的膜通常是获得干铸造后2个星期，在标准条件下的温度和压力（〜25℃，1个大气压）。组织接合的机理尚未完全清楚，但有人指出，RB扩散从进入相邻?...

<table border="1"><tbody><tr><td> 的试剂的名称 </td><td> 公司 </td><td> 目录编号 </td><td> 评论（可选） </td></tr><tr><td>孟加拉玫瑰红</td><td> Sigma-Aldrich公司</td><td> 632-69-9 </td><td></td></tr><tr><td>壳聚糖（MW中等） </td><td> Sigma-Aldrich公司</td><td> 10318AJ </td><td></td></tr><tr><td>冰醋酸</td><td> Sigma-Aldrich公司</td><td> 08050051 </td><td> 2％（体积/体积）在DI水</td></tr><tr><td>磁力搅拌器</td><td> Heidolph </td><td>陈喜混合小号</td><td></td></tr><tr><td>离心分离</td><td> Beckman Coulter公司</td><td> Allegra的X-12R </td><td></td></tr><tr><td>分光光度计</td><td>瓦里安</td><td>卡里4000紫外 - 可见</td><td></td></tr><tr><td>激光</td><td> CNI激光</td><td> MGL-532 </td><td></td></tr><tr><td>千分尺</td><td>三丰</td><td>系列227 </td><td></td></tr><tr><td>手术显微镜</td><td>蔡司</td><td> OPMI </td><td></td></tr></tbody></table>

fabrication and application of rose bengal-chitosan films in laser tissue repair

光化学组织结合（PTB）是一种无缝合的组织修复的技术，这是实现通过施加一个解决办法，玫瑰红（RB）之间的两个组织的边缘1,2。然后这些被选择性地吸收由RB通过激光照射。据说所得的光化学反应交联，在组织中的胶原纤维与最小的热量生产3。在这份报告中，RB已成立于薄的壳聚糖膜制造一个新的组织粘合剂，是激光激活。基于脱乙酰壳多糖和含有〜0.1％（重量）RB，粘接膜，制造和粘接小牛肠和大鼠胫神经由固态激光器（λ= 532nm的，注量〜110焦耳/厘米2，光斑大小〜0.5厘米） 。一个单一的列的张力计，与个人计算机连接，用于测试的接合强度。 RB-壳聚糖粘合剂粘合牢固，肠强度为15±6千帕（N = 30）。的粘接强度下降到2＆plusmn; 2千帕（= 30）时，激光不施加到粘合剂。胫神经的吻合，也可以不使用的缝合线的情况下完成。一种新型的壳聚糖胶粘剂已债券光化学组织制造的，不需要缝合。

Watch this Scientific Journal Video about Fabrication and Application of Rose Bengal-chitosan Films in Laser Tissue Repair at JoVE.com

Fabrication and Application of Rose Bengal-chitosan Films in Laser Tissue Repair

缝线在手术过程中，通常需要修复组织。然而，它们的应用可能是有问题的，因为它们是侵入性的，并且可能会损坏组织。新的组织粘合剂的制备及应用方法，在这里报道。该粘合膜的激光激活的，并且不需要使用缝线。

激光对生物组织的修复孟加拉玫瑰红壳聚糖膜的制备及应用

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