Name: fabrication and application of rose bengal-chitosan films in laser tissue repair
Uploaded: 2012-10-23T15:00:00
Description: Watch this Scientific Journal Video about Fabrication and Application of Rose Bengal-chitosan Films in Laser Tissue Repair at JoVE.com

Dit werk werd ondersteund door de UWS Research Grant.

1. Chitosan Lijm Voorbereiding <ol><li> De chitosan poeder is oplosbaar in azijnzuuroplossing, voor de bereiding van een voorraad oplossing van azijnzuur (2% v / v), 10 ml ijsazijn aan 490 ml gedeïoniseerd water (DI-H 2 O). </li><li> Voor de bereiding van een voorraadoplossing van Rose Bengal (RB) (0,01% w / v) in azijnzuur, wegen 5 mg van RB in een injectieflacon omwikkeld met aluminiumfolie om te voorkomen fotobleken. Voeg 0,5 ml van DI-H 2 O om het poeder op te lossen dan 49,5 ml van het azij...

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De roos lijm fabricage is gebaseerd op een eenvoudige droge-cast proces, hoewel het oplossen van RB in zure pH heeft langdurig roeren van de chitosan-oplossing. Het is belangrijk dat de oplossing drogen totdat het een in water onoplosbaar film. Dit gebeurt wanneer het gewicht watergehalte ~ 10% in de gedroogde film 6. Onoplosbare films worden gewoonlijk verkregen 2 weken na droog-casting bij standaardomstandigheden van temperatuur en druk (~ 25 ° C en ~ 1 atm). Het mechanisme van weefsel binding is nog niet ...

<table border="1"><tbody><tr><td> Naam van het reagens </td><td> Vennootschap </td><td> Catalogusnummer </td><td> Opmerkingen (optioneel) </td></tr><tr><td> Bengaals- </td><td> Sigma-Aldrich </td><td> 632-69-9 </td><td></td></tr><tr><td> Chitosan (medium MW) </td><td> Sigma-Aldrich </td><td> 10318AJ </td><td></td></tr><tr><td> IJsazijn </td><td> Sigma-Aldrich </td><td> 08050051 </td><td> 2% v / v in DI water </td></tr><tr><td> Magneetroerder </td><td> Heidolph </td><td> MR Hei-Mix S </td><td></td></tr><tr><td> Centrifugeer </td><td> Beckman Coulter </td><td> Allegra X-12R </td><td></td></tr><tr><td> Spectrofotometer </td><td> Varian </td><td> Cary 4000 UV-zichtbaar </td><td></td></tr><tr><td> Laser </td><td> CNI Laser </td><td> MGL-532 </td><td></td></tr><tr><td> Micrometer </td><td> Mitutoyo </td><td> Serie 227 </td><td></td></tr><tr><td> Operatiemicroscoop </td><td> Zeiss </td><td> OPMI </td><td></td></tr></tbody></table>

fabrication and application of rose bengal-chitosan films in laser tissue repair

Fotochemische weefsel bonding (PTB) is een techniek voor hechtingen weefselherstel, wat wordt bereikt door een oplossing Bengaals (RB) tussen twee weefselranden 1,2. Deze worden vervolgens bestraald door een laser die selectief wordt geabsorbeerd door de RB. De resulterende fotochemische reacties zou verknopen de collageenvezels in het weefsel met minimale warmteproductie 3. In dit rapport, is RB opgenomen in dunne chitosan films op een nieuw weefsel lijm dat is met een laser-geactiveerde fabriceren. Zelfklevende films, gebaseerd op chitosan en met 0,1 gew% ~ RB zijn vervaardigd en verbonden met kalf ingewanden en rat tibiale zenuwen door een vaste-stof laser (λ = 532 nm, Fluence ~ 110 J / cm 2, puntgrootte ~ 0,5 cm) . Een kolom tensiometer, gekoppeld aan een personal computer wordt gebruikt om de hechting te testen. De RB-chitosan lijmverbindingen stevig aan de darm met een sterkte van 15 ± 6 kPa (n = 30). De kleefkracht daalt tot 2 &amp;plusmn, 2 kPa (n = 30) wanneer de laser niet wordt toegepast op de kleefstof. De anastomose van scheenbeen zenuwen kunnen ook aangevuld worden zonder het gebruik van hechtingen. A novel chitosan lijm is vervaardigd dat obligaties fotochemisch aan weefsel en vereist geen hechtingen.

Watch this Scientific Journal Video about Fabrication and Application of Rose Bengal-chitosan Films in Laser Tissue Repair at JoVE.com

Fabrication and Application of Rose Bengal-chitosan Films in Laser Tissue Repair

Hechtingen zijn meestal nodig om weefsel te herstellen tijdens chirurgische procedures. Echter, de toepassing problematisch omdat ze invasief en tast weefsel. De fabricage en applicatie methoden van een nieuwe weefsel lijm worden hier gemeld. Deze kleeffolie laser geactiveerd en vereist niet het gebruik van hechtingen.

Fabricage en toepassing van Rose Bengal-chitosan Films in Laser Tissue Repair

Photochemical tissue bonding (PTB) is a sutureless technique for tissue repair, which is achieved by applying a solution of rose bengal (RB) between two ...

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