Name: fabrication and application of rose bengal-chitosan films in laser tissue repair
Uploaded: 2012-10-23T15:00:00
Description: Watch this Scientific Journal Video about Fabrication and Application of Rose Bengal-chitosan Films in Laser Tissue Repair at JoVE.com

This work was supported by the UWS Research Grant.

1. Chitosan Adhesive Preparation
<ol>
 <li>The chitosan powder is soluble in acetic acid solution; for the preparation of a stock solution of acetic acid (2% v/v), add 10 ml glacial acetic acid to 490 ml deionized water (DI-H2O).</li>
 <li>For the preparation of a stock solution of Rose Bengal (RB) (0.01% w/v) in acetic acid, weigh 5 mg of RB in a vial wrapped with aluminum foil to avoid photobleaching. Add 0.5 ml of DI-H2O to dissolve the powder then add 49.5 ml of the acetic acid stock solution ...

<ol>
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The rose adhesive fabrication is based on a simple dry-cast process, though the dissolution of RB in acidic pH requires prolonged stirring of the chitosan solution. It is important to let the solution dry up until it becomes a water-insoluble film. This happens when the weight water content is ~10% in the dried film6. Insoluble films are usually obtained 2 weeks after dry-casting at standard conditions of temperature and pressure (~25 &deg;C and ~1 atm). The mechanism of tissue bonding is not clear yet, althou...

<table border="1">
<tbody>
 <tr>
 <td>Name of the reagent</td>
 <td>Company</td>
 <td>Catalogue number</td>
 <td>Comments (optional)</td>
 </tr>
 <tr>
 <td>Rose Bengal</td>
 <td>Sigma-Aldrich</td>
 <td>632-69-9</td>
 <td></td>
 </tr>
 <tr>
 <td>Chitosan (medium MW)</td>
 <td>Sigma-Aldrich</td>
 <td>10318AJ</td>
 <td></td>
 </tr>
 <tr>
 <td>Glacial acetic acid</td>
 <td>Sigma-Aldrich</td>
 <td>08050051</td>
 <td>2% v/v in DI water</td>
 </tr>
 <tr>
 <td>Magnetic stirrer</td>
 <td>Heidolph</td>
 <td>MR Hei-Mix S</td>
 <td></td>
 </tr>
 <tr>
 <td>Centrifuge</td>
 <td>Beckman Coulter</td>
 <td>Allegra X-12R</td>
 <td></td>
 </tr>
 <tr>
 <td>Spectrophotometer</td>
 <td>Varian </td>
 <td>Cary 4000 UV-Visible</td>
 <td></td>
 </tr>
 <tr>
 <td>Laser</td>
 <td>CNI Laser </td>
 <td>MGL-532 </td>
 <td></td>
 </tr>
 <tr>
 <td>Micrometer</td>
 <td>Mitutoyo</td>
 <td>Series 227</td>
 <td></td>
 </tr>
 <tr>
 <td>Surgical 			microscope</td>
 <td>Zeiss </td>
 <td>OPMI</td>
 <td></td>
 </tr>
 </tbody>
</table>

fabrication and application of rose bengal-chitosan films in laser tissue repair

Photochemical tissue bonding (PTB) is a sutureless technique for tissue repair, which is achieved by applying a solution of rose bengal (RB) between two tissue edges1,2. These are then irradiated by a laser that is selectively absorbed by the RB. The resulting photochemical reactions supposedly crosslink the collagen fibers in the tissue with minimal heat production3. In this report, RB has been incorporated in thin chitosan films to fabricate a novel tissue adhesive that is laser-activated. Adhesive films, based on chitosan and containing ~0.1 wt% RB, are fabricated and bonded to calf intestine and rat tibial nerves by a solid state laser (&lambda;=532 nm, Fluence~110 J/cm2, spot size~0.5 cm). A single-column tensiometer, interfaced with a personal computer, is used to test the bonding strength. The RB-chitosan adhesive bonds firmly to the intestine with a strength of 15 &plusmn; 6 kPa, (n=30). The adhesion strength drops to 2 &plusmn; 2 kPa (n=30) when the laser is not applied to the adhesive. The anastomosis of tibial nerves can be also completed without the use of sutures. A novel chitosan adhesive has been fabricated that bonds photochemically to tissue and does not require sutures.

Watch this Scientific Journal Video about Fabrication and Application of Rose Bengal-chitosan Films in Laser Tissue Repair at JoVE.com

Fabrication and Application of Rose Bengal-chitosan Films in Laser Tissue Repair

Sutures are usually needed to repair tissue during surgical procedures. However, their application can be problematic as they are invasive and may damage tissue. The fabrication and application methods of a novel tissue adhesive are here reported. This adhesive film is laser-activated and does not require the use of sutures.

Photochemical tissue bonding (PTB) is a sutureless technique for tissue repair, which is achieved by applying a solution of rose bengal (RB) between two ...

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