Name: lensfree on-chip tomographic microscopy employing multi-angle illumination and pixel super-resolution
Uploaded: 2012-08-16T13:00:00
Description: Watch this Scientific Journal Video about Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution at JoVE.com

1. Imaging Setup LOT kan samles i et kompakt og let field-bærbar arkitektur 8, og alternativt som en optofluidic mikroskop med sektionsopdelt billeddannelse evne. 9 i denne rapport, men vi vil beskrive den grundlæggende billedbehandling opsætning af en bench-top implementering mod tomografi af statisk prøver. <ol><li> Belysning Modul: i Lot, kan delvist sammenhængende lyskilder såsom lysemitterende-dioder (LED) anvendes. For eksperimentel fleksi...

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	<li>Schmitt, J. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optical+coherence+tomography+(OCT):+a+review,+J.+Sel.+Top.">Optical coherence tomography (OCT): a review, J. Sel. Top.</a> Quant. Elect. 5, 1205-1215 (1999).</li><li>Keller, P. J., Schmidt, A. D., Wittbrodt, J., Stelzer, E. H. K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Reconstruction+of+zebrafish+early+embryonic+development+by+scanned+light+sheet+microscopy.">Reconstruction of zebrafish early embryonic development by scanned light sheet microscopy.</a> Science. 322, 1065-1069 (2008).</li><li>Sharpe, J., Ahlgren, U., Perry, P., Hill, B., Ross, A., Hecksher-S&#248;rensen, J., Baldock, R., Davidson, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optical+Projection+Tomography+as+a+Tool+for+3D+Microscopy+and+Gene+Expression+Studies.">Optical Projection Tomography as a Tool for 3D Microscopy and Gene Expression Studies.</a> Science. 296, 541-545 (2002).</li><li>Sung, Y., Choi, W., Fang-Yen, C., Badizadegan, K., Dasari, R. R., Feld, M. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optical+diffraction+tomography+for+high+resolution+live+cell+imaging.">Optical diffraction tomography for high resolution live cell imaging.</a> Opt. Exp. 17, 266-277 (2009).</li><li>Debailleul, M., Simon, B., Georges, V., Haeberle, O., Lauer, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Holographic+microscopy+and+diffractive+microtomography+of+transparent+samples.">Holographic microscopy and diffractive microtomography of transparent samples.</a> Meas. Sci. Technol. 19, 074009 (2008).</li><li>Charri&#232;re, F., Pavillon, N., Colomb, T., Depeursinge, C., Heger, T. J., Mitchell, E. A. D., Marquet, P., Rappaz, B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Living+specimen+tomography+by+digital+holographic+microscopy:+Morphometry+of+testate+amoeba.">Living specimen tomography by digital holographic microscopy: Morphometry of testate amoeba.</a> Opt. Exp. 14, 7005-7013 (2006).</li><li>Isikman, S. O., Bishara, W., Mavandadi, S., Yu, S. W., Feng, S., Lau, R., Ozcan, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lens-free+optical+tomographic+microscope+with+a+large+imaging+volume+on+a+chip.">Lens-free optical tomographic microscope with a large imaging volume on a chip.</a> Proc. Nat. Acad. Sci. 108, 7296-7301 (2011).</li><li>Isikman, S. O., Bishara, W., Sikora, U., Yaglidere, O., Yeah, J., Ozcan, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Field-portable+Lensfree+Tomographic+Microscope.">Field-portable Lensfree Tomographic Microscope.</a> Lab Chip. 11, 2222-2230 (2011).</li><li>Isikman, S. O., Bishara, W., Zhu, H., Ozcan, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optofluidic+tomography+on+a+chip.">Optofluidic tomography on a chip.</a> App. Phys. Lett. 98, 161109 (2011).</li><li>Bishara, W., Su, T. W., Coskun, A., Ozcan, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Lensfree+on-chip+microscopy+over+a+wide+field-of-view+using+pixel+super-resolution.">Lensfree on-chip microscopy over a wide field-of-view using pixel super-resolution.</a> Opt. Exp. 18, 11181-11191 (2010).</li><li>Messaoudi, C., Boudier, T., Sorzano, C. O. S., Marco, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=TomoJ:+tomography+software+for+three-dimensional+reconstruction+in+transmission+electron+microscopy.">TomoJ: tomography software for three-dimensional reconstruction in transmission electron microscopy.</a> BMC Bioinformatics. 8, 288 (2007).</li><li>Heng, X., Erickson, D., Baugh, L. R., Yaqoob, Z., Sternberg, P. W., Psaltis, D., Yang, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Optofluidic+Microscopy:+A+Method+for+Implementing+High+Resolution+Optical+Microscope+On+A+Chip.">Optofluidic Microscopy: A Method for Implementing High Resolution Optical Microscope On A Chip.</a> Lab on a Chip. 6, 1274-1276 (2006).</li></ol>

Det er vigtigt at understrege, at den unikke geometri lensfree on-chip holografisk mikroskopi er den kritiske enabler for at opnå pixel super-opløsning og tomografisk billeddannelse. Da de optagede billeder ikke antages at være projektion billeder som i usammenhængende kontakt billedbehandling nærmer sig 12, men projektion hologrammer og diffraktion af det transmitterede lys, indtil den rammer detektoren kan digitalt korrigeres ved holografisk genopbygning. Derfor kan variationer i prøve-til-se...

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lensfree on-chip tomographic microscopy employing multi-angle illumination and pixel super-resolution

Tomografisk scanning har været et almindeligt anvendte værktøj i medicin som kan tilvejebringe tredimensionale (3D) strukturel information om genstande af forskellig størrelse skalaer. I mikrometer og millimeter skalaer, finde optisk mikroskopi modaliteter stigende brug på grund af ikke-ioniserende karakter af synligt lys, og tilgængeligheden af ​​en bred vifte af belysning kilder (såsom lasere og lysemitterende-dioder) og påvisning elementer (såsom stort format CCD og CMOS-detektor-arrays). Blandt de nyligt udviklede optiske tomografiske mikroskopi nærmere, kan man inkludere optisk kohærens tomografi, optisk diffraktion tomografi, optisk projektion tomografi og lys-ark mikroskopi. 1-6 Disse platforme giver i snit billeddannelse af celler, mikroorganismer og model dyr, såsom C. elegans, zebrafisk og musefostre. Eksisterende 3D optiske kameraer har generelt forholdsvis klodsede og kompleks arkitektur, hvilket begrænser the tilgængeligheden af ​​disse udstyr til avancerede laboratorier, og hæmmer deres integration med lab-on-a-chip platforme og mikrofluid chips. At give et alternativ tomografisk mikroskop, vi for nylig udviklet lensfree optisk tomografi (LOT) som en high-throughput, kompakt og omkostningseffektiv optisk tomografi modalitet. 7 LOT udsmid brugen af linser og voluminøse optiske komponenter, og i stedet bygger på multi-vinkel belysning og digital beregning for at opnå dybde løst billeder af mikro-objekter over en stor billedbehandling volumen. Meget kan billedet biologisk prøve med en rumlig opløsning på under 1 um x &lt;1 um x &lt;3 um i x, y og z dimensioner, henholdsvis over et stort billeddannelse mængde fra 15 til 100 mm3, og kan være særligt nyttige til lab-on-a-chip platforme.

Watch this Scientific Journal Video about Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution at JoVE.com

Lensfree On-chip Tomographic Microscopy Employing Multi-angle Illumination and Pixel Super-resolution

Lensfree optisk tomografi er en tredimensional mikroskopi teknik, som giver en rumlig opløsning på under 1 um x &lt;1 um x &lt;3 um x, y og z dimensioner henholdsvis over et stort billeddannelse-volumen fra 15 til 100 mm<sup&gt; 3</sup&gt;, Som kan være særligt nyttige til integration med lab-on-en-chip platforme.

Lensfree On-chip tomografiske Microscopy Under anvendelse af Multi-vinkel belysning og Pixel Super-resolution

Tomographic imaging has been a widely used tool in medicine as it can provide three-dimensional (3D) structural information regarding objects of different ...

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