Sign In

JoVE Journal

Medicine

This content is Open Access.

Improved Visualization of Lung Metastases at Single Cell Resolution in Mice by Combined In-situ Perfusion of Lung Tissue and X-Gal Staining of lacZ-Tagged Tumor Cells

17.1K Views

08:47 min

August 21st, 2012

August 21st, 2012

17,139 Views

0:05

Title

1:12

In-situ Lung Perfusion and Fixation

3:30

Visualizing lacZ-tagged Metastatic Tumor Cells in Entire Lung Lobes

4:31

Visualizing lacZ-tagged Metastatic Tumor Cells in Lung Cryosections

5:48

Metastases Analysis in Dunn/LM8 Osteosarcoma-bearing Mice

8:00

Conclusion

The novel protocol reported in the present study allows selective detection of lung metastases at single cell resolution in mice by combined in-situ lung perfusion and fixation and X-Gal staining of lacZ-tagged tumor cells.

We use cookies to enhance your experience on our website.

By continuing to use our website or clicking “Continue”, you are agreeing to accept our cookies.

Learn More