资金由美国国家科学基金会通过NUE工程和领导的联盟程序表示感谢。

1。胶体银奈米粒子的合成克莱顿法（略作修改，廉价的）22的反应机制中很详细的描述，在辅助信息的参考帕维尔et.al一起的不需要的水解侧的NaBH 4，在室温或更高温度下反应23 <ol><li> 12-24小时，在10％HNO 3的浴，然后清洁所有的玻璃器皿为4-12小时，在1.25 M的NaOH在40％的乙醇浴中，最后的高压釜。玻璃器皿应彻底漂洗最低五次后，用超纯水（1...

<ol>
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胶体银奈米粒子的紫外 - 可见吸收光谱法和拉曼光谱这是众所周知的，数量的表面等离子体共振一种胶体的吸收光谱的峰的对称性的AGNPS增加而减小。此外，AgNP聚合导致的外观更广泛或红移的峰。25,26的存在下，在394 nm处的一个单一的，尖锐的和对称的SPR峰值表示中度聚集和粒度分布的小的，球形的银奈米粒子。 前和超滤后的胶体样品的纯度，证明...

<table border="1"><tr><td>硝酸银（AgNO 3的 ） </td><td> Acros Organics公司</td><td> CAS：7761-88-8 </td><td></td></tr><tr><td>硼氢化钠（加入NaBH 4） </td><td> Acros Organics公司</td><td> CAS：16940-66-2 </td><td></td></tr><tr><td>硝酸（HNO 3，远舰） </td><td>默飞世尔科技公司</td><td> A467-1 </td><td> ICP分析痕量金属级</td></tr><tr><td> 10,000微克毫升-1银标准，EnviroConcentrate </td><td>超科学</td><td>美IAA-047 </td><td></td></tr><tr><td> KrosFlo研究（二） 我切向流过滤系统</td><td>频谱检测实验室公司</td><td> <a href="http://www.spectrumlabs.com/pshop/RequestPrice/index.html?LiFrom=%2Ffiltration%2FKR2SystemO.html;FrName=Research+IIi+System;Pn=SYR2-U20-01N;" target="_blank">SYR2-U20-01N</a> </td><td></td></tr><tr><td> 0.05微米PS（0.5毫米）460厘米</td><td>频谱检测实验室公司</td><td> <a href="http://www.spectrumlabs.com/filtration/MidiKros.html?Pn=X30S-900-02N;" target="_blank">X30S-900-02N</a> </td><td></td></tr><tr><td>南部100 kD的PS 200平方厘米 </td><td>频谱检测实验室公司</td><td> X3-100S-901-02N </td><td></td></tr><tr><td> micro100 kD的PS 20 cm 2的 </td><td>频谱检测实验室公司</td><td> X1AB-300-10N </td><td></td></tr><tr><td> MASTERFLEX的C-Flex管L / S尺寸17 </td><td>科尔 - 帕默仪器有限公司</td><td> 06424-17 </td><td></td></tr><tr><td> MASTERFLEX的C-Flex管L / S尺寸14 </td><td>科尔 - 帕默仪器有限公司</td><td> 06424-14 </td><td></td></tr><tr><td>卡里50 UV-VIS-NIR分光光度计</td><td>瓦里安公司</td><td></td><td></td></tr><tr><td>的LabRam HR 800系统</td><td> HORIBA Jobin Yvon公司公司</td><td></td><td></td></tr><tr><td>瓦里安710ES ICP-OES </td><td>瓦里安公司</td><td></td><td></td></tr></table> 表1中。具体的试剂和设备。

tangential flow ultrafiltration: a  &ldquo;green&rdquo; method for the size selection and concentration of colloidal silver nanoparticles

如今，AGNPS被广泛用于制造消费类产品，水消毒剂，2疗法，1，3和生物医学设备由于其强大的抗菌性能。3-6这些纳米粒子的应用，我们强烈的AgNP大小和聚集状态的影响。存在许多挑战在受控制造7和未官能化的，同质的银奈米粒子从化学侵蚀性的上限/稳定剂或有机溶剂是免费的基于大小的隔离4,8 7-13摆脱限制成本高的毒性试剂，或减少的AgNP合成或分离方法（ 例如 ，离心，尺寸相关的溶解度，尺寸排阻色谱法，等）的效率。10,14-18为了克服这一点，我们最近发现，TFU允许更大的控制权的大小，浓度和（300克赖顿农业非点源污染的聚集态毫升15.3微克毫升-1 198.7微克毫升-1）下降到10毫升的19比传统的隔离方法，如超速离心。 TFU是常用的重量为基础的分离的蛋白质，病毒和细胞的再循环方法。20,21简单地说，该液体样品通过中空纤维膜的孔径范围从1,000 kD的10 kD的一系列。较小的悬浮或溶解的样品中的成分将通过多孔隔板与溶剂（滤液）一起，而较大的成分被保留（滞留）。 TFU可能被认为是“绿色”的方法，因为它既不损害样品，也不需要额外的溶剂，以消除有毒过量的试剂和副产品。此外，TFU可能被应用到了大量的各种纳米粒子，作为疏水性和亲水性两种过滤器是可用的。 本研究的两个主要目标是：1）说明实验方面的的TFU方法通过一个被邀请的视频体验和2）表现出较大的胶体纳米粒子的体积和更小的体积，截留TFU方法的可行性。首先，unfuctionalized银奈米粒子（4升，15.2微克毫升-1）的合成通过用NaBH 4还原的AgNO 3使用既定克莱顿方法22,23。 AgNP多分散性，然后最小化通过使用50纳米的过滤器（460厘米2），以除去银奈米粒子和AgNP-聚集体由两个100 kD的滤波器（200厘米2和20厘米2）大于50nm，接着的3个步骤TFU集中银奈米粒子。有代表性的样品进行了表征，利用透射电子显微镜，紫外 - 可见吸收光谱法，拉曼光谱法，电感耦合等离子体发射光谱。最终阻滞高度集中（4毫升，8,539.9微克毫升-1），但卑微的汇总和均匀银奈米粒子为1-20纳米的直径。这对应于约62％的银浓度收率。

Watch this Scientific Journal Video about Tangential Flow Ultrafiltration: A  Ã¢â‚¬Å“GreenÃ¢â‚¬Â Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles at JoVE.com

Tangential Flow Ultrafiltration: A  ÃƒÂ¢Ã¢â€šÂ¬Ã…â€œGreenÃƒÂ¢Ã¢â€šÂ¬Ã‚Â Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles

切向流超滤（TFU），是一个循环的生物样品的重量为基础的分离方法。 TFU适于大小选择（1-20 nm直径）和高度集中大量的多分散性的银纳米颗粒（4 L的15.2微克毫升<sup&gt; -1</sup8,539.9微克毫升下降到4毫升<sup&gt; -1</sup&gt;），以最小的聚集。

切向流超：A胶体银纳米粒子的大小选择与集中“绿色”方法

Nowadays, AgNPs are extensively used in the manufacture of consumer products,1 water disinfectants,2 therapeutics,1, 3 and biomedical devices4 due to ...

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Tangential Flow Ultrafiltration: A  &ldquo;Green&rdquo; Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles

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Development of a Colloidal Gold-based Immunochromatographic Test Strip for Detection of Cetacean Myoglobin

对肌红蛋白鲸类检测基于金免疫层析胶体试纸条的研制

This protocol describes the development of a colloidal gold immunochromatographic test strip based on the sandwich format that can be used to ...

Filtration Isolation of Nucleic Acids:  A Simple and Rapid DNA Extraction Method

核酸的分离过滤：一种简单快速的DNA提取方法

FINA, filtration isolation of nucleic acids, is a novel extraction method which utilizes vertical filtration via a separation membrane and absorbent pad ...