Finansiering fra National Science Foundation gennem NUE i Engineering og Leader Consortium Programmer er modtaget med tak.

1. Syntese af Kolloide AgNPs Reaktionsmekanismen for Creighton metoden (let modificeret, billig) 22 er beskrevet i detaljer i Hjælpeoplysninger reference Pavel et.al sammen med den uønskede hydrolyse sidereaktion af NaBH4 ved stuetemperatur eller højere. 23 <ol><li> Rens alt glasudstyr i 12-24 timer i en 10% HNO 3 bad, derefter i 4 til 12 timer i en 1,25 M NaOH i 40% ethanol-bad, og til slut autoklaven. Glasgenstande bør skylles grundigt mind...

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UV-Vis absorptionsspektrofotometri og Raman spektroskopi af kolloide AgNPs Det er velkendt, at antallet af overfladeplasmonresonans toppe i absorptionsspektret for en kolloid aftager, når symmetrien mellem de AgNPs forøges. Derudover AgNP aggregation fører til fremkomsten af bredere eller rød-skiftede toppe. 25,26 Tilstedeværelsen af et enkelt, skarp og symmetrisk SPR top ved 394 nm er tegn på små, sfæriske AgNPs af moderat aggregering og størrelsesfordeling. <p class="j...

<table border="1"><tr><td> Sølvnitrat (AgNO 3) </td><td> Acros Organics Inc. </td><td> CAS: 7761-88-8 </td><td></td></tr><tr><td> Natriumborhydrid (NaBH4) </td><td> Acros Organics Inc. </td><td> CAS: 16940-66-2 </td><td></td></tr><tr><td> Salpetersyre (HNO 3, Optima) </td><td> Fisher Scientific Inc. </td><td> A467-1 </td><td> Trace metal kvalitet til ICP-analyse </td></tr><tr><td> 10.000 ug ml-1 sølv standard, EnviroConcentrate </td><td> Ultra Scientific </td><td> US-IAA-047 </td><td></td></tr><tr><td> KrosFlo Research II i tangentielstrømningsfiltrering System </td><td> Spectrum Laboratories Inc. </td><td> <a href="http://www.spectrumlabs.com/pshop/RequestPrice/index.html?LiFrom=%2Ffiltration%2FKR2SystemO.html;FrName=Research+IIi+System;Pn=SYR2-U20-01N;" target="_blank">SYR2-U20-01N</a> </td><td></td></tr><tr><td> 0,05 um PS (0,5 mm) 460 cm2 </td><td> Spectrum Laboratories Inc. </td><td> <a href="http://www.spectrumlabs.com/filtration/MidiKros.html?Pn=X30S-900-02N;" target="_blank">X30S-900-02N</a> </td><td></td></tr><tr><td> Midi 100 kD PS 200 cm 2 </td><td> Spectrum Laboratories Inc. </td><td> X3-100S-901-02N </td><td></td></tr><tr><td> Micro100 kD PS 20 cm2 </td><td> Spectrum Laboratories Inc. </td><td> X1AB-300-10N </td><td></td></tr><tr><td> MASTERFLEX C-Flex slange L / S størrelse 17 </td><td> Cole-Palmer Instrument Co </td><td> 06.424-17 </td><td></td></tr><tr><td> MASTERFLEX C-Flex slange L / S Størrelse 14 </td><td> Cole-Palmer Instrument Co </td><td> 06.424-14 </td><td></td></tr><tr><td> Cary 50 UV-VIS-NIR spektrofotometer </td><td> Varian Inc. </td><td></td><td></td></tr><tr><td> LabRam HR 800-systemet </td><td> Horiba Jobin Yvon Inc. </td><td></td><td></td></tr><tr><td> Varian 710ES ICP-OES </td><td> Varian Inc. </td><td></td><td></td></tr></table> Tabel 1. Specifikke reagenser og udstyr.

tangential flow ultrafiltration: a  &ldquo;green&rdquo; method for the size selection and concentration of colloidal silver nanoparticles

I dag er AgNPs flittigt brugt til fremstilling af forbrugsgoder, 1 vand desinfektionsmidler, 2 terapi, 1, 3 og biomedicinsk udstyr 4 på grund af deres stærke antimikrobielle egenskaber. 3-6 Disse nanopartikler applikationer er kraftigt påvirket af den AgNP størrelse og sammenlægning tilstand . Mange udfordringer findes i den styrede fremstilling 7 og størrelse baseret isolation 4,8 af ikke-funktionaliseret, homogene AgNPs der er fri for kemisk aggressive capping / stabiliseringsmidler eller organiske opløsningsmidler. 7-13 Begrænsninger komme ud toksiciteten af reagenser, høje omkostninger og reduceret effektiviteten af de AgNP syntese eller isolering metoder (f.eks, centrifugering, størrelse-afhængig opløselighed, gelpermeationskromatografi, etc.). 10,14-18 For at overvinde dette har vi for nylig vist, at TFU tillader større kontrol over størrelsen, koncentrationen og sammenlægning tilstand af Creighton AgNPs (300ml 15,3 ug ml-1 ned til 10 ml 198,7 ug ml-1) end konventionelle fremgangsmåder til isolation, såsom ultracentrifugering. 19 TFU er en recirkulation, der almindeligvis anvendes til den vægtbaserede isolering af proteiner, vira og celler. 20,21 Kort fortalt anvendes den flydende prøve ledes gennem en serie af hulfibermembraner med en porestørrelse i området fra 1000 kD til 10 kD. Mindre suspenderet eller opløst i prøven vil passere gennem den porøse barriere sammen med opløsningsmidlet (filtrat), medens de større bestanddele bevares (retentat). TFU kan betragtes som en &quot;grøn&quot; metode, da det hverken skader prøve heller ikke kræver yderligere opløsningsmiddel til at fjerne giftige overskydende reagenser og biprodukter. Endvidere kan TFU anvendes på et stort udvalg af nanopartikler som både hydrofobe og hydrofile filtre findes. De to vigtigste mål med denne undersøgelse var: 1) at illustrerede eksperimentelle aspekter af TUF tilgang gennem en indbudt video oplevelse og 2) at demonstrere muligheden for at TUF metoden til større mængder kolloide nanopartikler og mindre mængder af retentat. Første, unfuctionalized AgNPs (4 L, 15,2 ug ml-1) blev syntetiseret under anvendelse af veletablerede Creighton metoden 22,23 ved reduktion af AgNO 3 med NaBH4. AgNP polydispersitet blev derefter minimeret via en 3-trins TFU anvendelse af en 50-nm filter (460 cm2) for at fjerne AgNPs og AgNP-aggregater større end 50 nm, efterfulgt af to 100-kD (200 cm 2 og 20 cm 2) filtre at koncentrere de AgNPs. Repræsentative prøver blev karakteriseret ved hjælp af transmissionselektronmikroskopi, UV-Vis absorptionsspektrofotometri, Raman spektroskopi, og induktiv koblet optisk emissionsspektroskopi. Det endelige retentat bestod af stærkt koncentreret (4 ml, 8,539.9 ug ml-1) endnu ringe aggregerede og homogenAgNPs på 1-20 nm i diameter. Dette svarer til en sølvkoncentration udbytte på ca 62%.

Watch this Scientific Journal Video about Tangential Flow Ultrafiltration: A  Ã¢â‚¬Å“GreenÃ¢â‚¬Â Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles at JoVE.com

Tangential Flow Ultrafiltration: A  ÃƒÂ¢Ã¢â€šÂ¬Ã…â€œGreenÃƒÂ¢Ã¢â€šÂ¬Ã‚Â Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles

Tangentiel strømnings-ultrafiltrering (TFU) er en recirkulation metode, der anvendes til den vægtbaserede adskillelse af biosamples. TFU blev tilpasset til størrelsen-select (1-20 nm diameter) og yderst koncentreret en stor mængde polydisperse sølvnanopartikler (4 L på 15,2 ug ml<sup&gt; -1</sup&gt; Ned til 4 ml 8,539.9 ug ml<sup&gt; -1</sup&gt;) Med minimal aggregering.

Tangential Flow Ultrafiltrering: &quot;Grønt&quot; Metode til størrelse udvælgelse og koncentration af kolloidt sølv Nanopartikler

Nowadays, AgNPs are extensively used in the manufacture of consumer products,1 water disinfectants,2 therapeutics,1, 3 and biomedical devices4 due to ...

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Tangential Flow Ultrafiltration: A  &ldquo;Green&rdquo; Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles

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