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1. Sintesi di AgNPs colloidale Il meccanismo di reazione per il metodo Creighton (leggermente modificato, poco costoso) 22 è descritto in dettaglio nelle informazioni di supporto di riferimento Pavel et.al insieme con l&#39;idrolisi indesiderato laterale reazione di NaBH 4 a temperatura ambiente o superiore. 23 <ol><li> Pulire la vetreria per 12-24 ore a 10% in HNO 3 bagno, poi per 4-12 ore in un 1,25 M NaOH in bagno di etanolo al 40%, e infine ...

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UV-Vis spettrofotometria di assorbimento e la Spettroscopia Raman di AgNPs colloidale E &#39;noto che il numero di picchi di risonanza plasmonica di superficie nello spettro di assorbimento di un colloide diminuisce la simmetria degli aumenti AgNPs. Inoltre, AgNP aggregazione porta alla comparsa dei picchi più ampi o rosso-spostato. 25,26 La presenza di un unico tagliente e simmetrica SPR picco a 394 nm è indicativo di piccole AgNPs sferiche di aggregazione moderata e distribuzione...

<table border="1"><tr><td> Di nitrato d&#39;argento (AgNO 3) </td><td> Acros Organics Inc. </td><td> CAS: 7761-88-8 </td><td></td></tr><tr><td> Sodio boroidruro (NaBH4) </td><td> Acros Organics Inc. </td><td> CAS: 16940-66-2 </td><td></td></tr><tr><td> L&#39;acido nitrico (HNO 3, Optima) </td><td> Fisher Scientific Inc. </td><td> A467-1 </td><td> Trace grado, metallo per l&#39;analisi ICP </td></tr><tr><td> 10.000 mg ml -1 argento standard, EnviroConcentrate </td><td> Ultra Scientific </td><td> US-IAA-047 </td><td></td></tr><tr><td> KrosFlo ricerca II i tangenziale sistema di filtrazione di flusso </td><td> Spectrum Laboratories Inc. </td><td> <a href="http://www.spectrumlabs.com/pshop/RequestPrice/index.html?LiFrom=%2Ffiltration%2FKR2SystemO.html;FrName=Research+IIi+System;Pn=SYR2-U20-01N;" target="_blank">SYR2-U20-01N</a> </td><td></td></tr><tr><td> 0,05 micron PS (0,5 mm) 460 cm 2 </td><td> Spectrum Laboratories Inc. </td><td> <a href="http://www.spectrumlabs.com/filtration/MidiKros.html?Pn=X30S-900-02N;" target="_blank">X30S-900-02N</a> </td><td></td></tr><tr><td> Midi 100 kD PS 200 cm 2 </td><td> Spectrum Laboratories Inc. </td><td> X3-100S-901-02N </td><td></td></tr><tr><td> Micro100 kD PS 20 cm 2 </td><td> Spectrum Laboratories Inc. </td><td> X1AB-300-10N </td><td></td></tr><tr><td> Masterflex C-Flex tubi L / S Dimensioni 17 </td><td> Cole-Palmer Instrument Co. </td><td> 06424-17 </td><td></td></tr><tr><td> Masterflex C-Flex tubi L / S Dimensioni 14 </td><td> Cole-Palmer Instrument Co. </td><td> 06424-14 </td><td></td></tr><tr><td> Cary 50 UV-VIS-NIR spettrofotometro </td><td> Varian Inc. </td><td></td><td></td></tr><tr><td> LabRam HR 800 Sistema </td><td> Horiba Jobin Yvon Inc. </td><td></td><td></td></tr><tr><td> 710ES Varian ICP-OES </td><td> Varian Inc. </td><td></td><td></td></tr></table> Tabella 1. Reagenti e attrezzature specifiche.

tangential flow ultrafiltration: a  &ldquo;green&rdquo; method for the size selection and concentration of colloidal silver nanoparticles

Oggi, AgNPs sono ampiamente utilizzati nella fabbricazione di prodotti di consumo, disinfettanti acqua 1, 2 terapeutiche, 1, 3 e 4 dispositivi biomedicali causa delle loro proprietà antimicrobiche potenti. 3-6 Queste applicazioni nanoparticelle sono fortemente influenzati dalla dimensione AgNP e stato di aggregazione . Esistono molte sfide nella fabbricazione controllato 7 e basato sulle dimensioni di isolamento 4,8 unfunctionalized, AgNPs omogenee che sono esenti da chimicamente aggressivi incappucciamento / agenti stabilizzanti o solventi organici. 7-13 Limitazioni emergere dalla tossicità dei reagenti, costi elevati o ridotti efficienza dei metodi di sintesi AgNP o isolamento (ad esempio, centrifugazione, dipendente dalle dimensioni solubilità, cromatografia ad esclusione dimensionale, ecc). 10,14-18 Per superare questo, abbiamo recentemente dimostrato che TFU permette un maggiore controllo sulla concentrazione di dimensione, e stato di aggregazione Creighton AgNPs (300ml di 15,3 pg ml -1 fino a 10 ml di 198,7 mg ml -1) rispetto ai metodi convenzionali di isolamento come ultracentrifugazione. 19 TFU è un metodo comunemente utilizzato per il ricircolo del peso basata isolamento di proteine, virus e cellule. 20,21 Brevemente, il campione liquido viene fatto passare attraverso una serie di membrane a fibre cave con dimensione dei pori compresa tra 1000 kD e 10 kD. Piccoli componenti sospesi o disciolti nel campione passa attraverso la membrana porosa con il solvente (filtrato), mentre i componenti più grandi vengono trattenuti (ritentato). TFU può essere considerato un metodo &quot;verde&quot; man mano che il campione né danni, né richiede ulteriore solvente tossico per eliminare i reagenti in eccesso e sottoprodotti. Inoltre, TFU può essere applicato ad una grande varietà di nanoparticelle come entrambi i filtri idrofobe e idrofile sono disponibili. I due obiettivi principali di questo studio sono stati: 1) per illustraregli aspetti sperimentali dell&#39;approccio TFU attraverso un&#39;esperienza video invitati e 2) per dimostrare la fattibilità del metodo TFU per grandi quantità di nanoparticelle colloidali e quantità inferiori di ritentato. Primi, AgNPs unfuctionalized (4 L, 15,2 pg ml -1) sono stati sintetizzati utilizzando il consolidato metodo Creighton 22,23 dalla riduzione di AgNO 3 con NaBH 4. AgNP polidispersità è stato poi minimizzato attraverso una 3-step TFU utilizzando un 50-nm filtro (460 cm 2) per rimuovere AgNPs e AgNP-aggregati maggiore di 50 nm, seguite da due 100-kD (200 cm 2 e 20 cm 2) filtri per concentrare le AgNPs. I campioni rappresentativi sono stati caratterizzati utilizzando la microscopia elettronica a trasmissione, spettrofotometria UV-Vis di assorbimento, spettroscopia Raman, e plasma ad accoppiamento induttivo spettroscopia ottica. Il ritenuto finale consisteva in alta concentrazione (4 ml, 8,539.9 mg ml -1) ma umile aggregata e omogeneaAgNPs di 1-20 nm di diametro. Questo corrisponde ad un rendimento argento concentrazione di circa 62%.

Watch this Scientific Journal Video about Tangential Flow Ultrafiltration: A  Ã¢â‚¬Å“GreenÃ¢â‚¬Â Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles at JoVE.com

Tangential Flow Ultrafiltration: A  ÃƒÂ¢Ã¢â€šÂ¬Ã…â€œGreenÃƒÂ¢Ã¢â€šÂ¬Ã‚Â Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles

Ultrafiltrazione a flusso tangenziale (TFU) è un metodo utilizzato per il ricircolo peso separazione basata su biosamples. TFU è stato adattato alle dimensioni-select (1-20 nm di diametro) e molto concentrato un grande volume di polidispersi nanoparticelle d&#39;argento (4 L di 15,2 ml mg<sup&gt; -1</sup&gt; Fino a 4 ml di 8,539.9 ml mcg<sup&gt; -1</sup&gt;) Con aggregazione minima.

Flusso tangenziale Ultrafiltrazione: un metodo &quot;verde&quot; per la selezione del formato e la concentrazione di nanoparticelle di argento colloidale

Nowadays, AgNPs are extensively used in the manufacture of consumer products,1 water disinfectants,2 therapeutics,1, 3 and biomedical devices4 due to ...

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Tangential Flow Ultrafiltration: A  &ldquo;Green&rdquo; Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles

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