엔지니어링 및 LEADER 컨소시엄 프로그램에 NUE을 통해 국립 과학 재단 (National Science Foundation)의 자금 지원은 굉장히 인정됩니다.

1. 콜로이드 AgNPs의 합성 크 레이튼 방법 (약간 수정, 저렴한) 22에 대한 반응 메커니즘은 원치 않는 가수 분해 실온 이상에서 NaBH 4 쪽 반응과 함께 참조 파벨 et.al의 지원 정보에 큰 자세히 설명되어 있습니다. 23 <ol><li> 4-12 40 % 에탄올 욕조에서 1.25 M NaOH의 시간, 그리고 마지막으로 압력솥 다음은 10 % HNO 3 목욕탕에 12-24 시간에 대한 모든 유리를...

<ol>
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UV-VIS 흡수 분광 광도법 및 콜로이드 AgNPs의 라만 분광학 이곳은 콜로이드의 흡수 스펙트럼의 표면 plasmon 공명 봉우리의 수는 AgNPs 증가 대칭으로 감소하는 것으로 알려져 있습니다. 또한, AgNP 집계는 광범위한이나 붉은 이동 봉우리의 모양이 생깁니다. 25,26은 394 nm의에서 단일 샤프하고 대칭 SPR 피크의 존재가 적당한 집계 및 크기 분포의 작은 구형 AgNPs을 나타내는 것입?...

<table border="1"><tr><td> 실버 질산염 (AgNO 3) </td><td> Acros 유기물 주식회사 </td><td> CAS : ​​7761-88-8 </td><td></td></tr><tr><td> 나트륨 borohydride (NaBH 4) </td><td> Acros 유기물 주식회사 </td><td> CAS : ​​16940-66-2 </td><td></td></tr><tr><td> 질산 (HNO 3, Optima) </td><td> 피셔 과학 주식회사 </td><td> A467-1 </td><td> ICP 분석을위한 금속 등급을 추적 </td></tr><tr><td> 10,000 μg ML -1은 표준 EnviroConcentrate </td><td> 울트라 과학 </td><td> US-IAA-047 </td><td></td></tr><tr><td> KrosFlo 연구 II 전 접선 흐름 여과 시스템 </td><td> 스펙트럼 연구소 주식회사 </td><td> <a href="http://www.spectrumlabs.com/pshop/RequestPrice/index.html?LiFrom=%2Ffiltration%2FKR2SystemO.html;FrName=Research+IIi+System;Pn=SYR2-U20-01N;" target="_blank">SYR2-U20-01N</a> </td><td></td></tr><tr><td> 0.05 μm PS (0.5 mm) 460cm 2 </td><td> 스펙트럼 연구소 주식회사 </td><td> <a href="http://www.spectrumlabs.com/filtration/MidiKros.html?Pn=X30S-900-02N;" target="_blank">X30S-900-02N</a> </td><td></td></tr><tr><td> 미디 100 KD PS 200cm 2 </td><td> 스펙트럼 연구소 주식회사 </td><td> X3-100S-901-02N </td><td></td></tr><tr><td> Micro100 KD PS 20cm이 </td><td> 스펙트럼 연구소 주식회사 </td><td> X1AB-300-10N </td><td></td></tr><tr><td> MasterFlex C-플렉스 튜브 L / S 사이즈 17 </td><td> 콜 - 팔머 인스트루먼트 (주) </td><td> 06424-17 </td><td></td></tr><tr><td> MasterFlex C-플렉스 튜브 L / S 사이즈 14 </td><td> 콜 - 팔머 인스트루먼트 (주) </td><td> 06424-14 </td><td></td></tr><tr><td> 캐리 50 UV-VIS-NIR 분광 광도계 </td><td> Varian 주식회사 </td><td></td><td></td></tr><tr><td> LabRam HR 800 시스템 </td><td> Horiba Jobin Yvon 주식회사 </td><td></td><td></td></tr><tr><td> Varian 710ES ICP-것들 </td><td> Varian 주식회사 </td><td></td><td></td></tr></table> 표 1. 특정 시약 및 장비.

tangential flow ultrafiltration: a  &ldquo;green&rdquo; method for the size selection and concentration of colloidal silver nanoparticles

현재, AgNPs이 광범위하게 자신의 강력한 항균 특성으로 인해 소비자 제품, 1 물 소독제, 2 치료학, 1, 3, 생물 의학 장치 (4)의 제조에 사용됩니다. 3-6이 nanoparticle 응용 프로그램을 강력하게 AgNP 크기와 집합 상태에 의해 영향을받습니다 . 많은 도전 제어 제조 7 캡핑 / 대리인 또는 유기 용제를 안정화 화학적으로 공격적인에서 무료로 제공되는 unfunctionalized, 동질 AgNPs의 크기 기반 격리 4,8에 존재합니다. 7-13 제한이 시약의 독성에서 높은 비용을 등장하거나 감소 AgNP 합성 또는 분리 방법 (예를 들어, 원심 분리, 크기에 따라 달라 용해도, 크기 배제 크로마토 그래피 등)의 효율성. 10,14-18이를 극복하기 위해, 우리는 최근 TFU 더 큰 크기, 농도 제어 등을 허용 것을 보여 주었다 크 레이튼 AgNPs (300 집계 상태같은 ultracentrifugation 등의 절연이 기존의 방법보다 198.7 μg ML -1)의 15.3 μg ML -1의 ML 다운 10 ML. 19 TFU은 일반적으로 단백질, 바이러스, 세포의 무게 기반의 절연에 사용되는 재순환 방식입니다. 간단히 20,21, 액체 샘플 KD 1,000에서 10 KD에 이르기까지 다양한 기공 크기와 중공 섬유 멤브레인의 일련을 통해 전달됩니다. 큰 성분은 (retentate) 유지하는 동안 예제에서 작은 중지 또는 해산 성분은 (여과) 용매와 함께 다공성 장벽을 전달합니다. TFU이가도 손해 샘플을로 &quot;녹색&quot;방법으로 간주하거나 독성 초과 시약 및 부산물을 제거 할 추가는 용매 필요 할 수 있습니다. 모두 소수성과 친수성 ​​필터를 사용할 수 있습니다대로 또한, TFU은 나노 입자의 다양한 적용 할 수 있습니다. 본 연구의 두 가지 주요 목표는 있었다 : 1) 설명하기초대 비디오 경험과 2를 통해 TFU 접근 방식의 실험 부분)은 콜로이드 나노 입자와 retentate의 작은 볼륨의 큰 볼륨에 대한 TFU 방법의 타당성을 입증합니다. 첫째, unfuctionalized AgNPs는 (4 L, 15.2 μg ML -1) NaBH 4 AgNO 3의 감소에 의해 잘 알려진 크 레이튼 방법 22,23을 사용하여 합성 하였다. AgNP polydispersity는 두개를 100 KD (200cm 2, 20cm 2) 필터에 의해 다음 AgNPs 및 AgNP-집계 큰 50 나노 미터를 제거하기 위해 50 나노 필터를 (460cm 2)를 사용하여 3 단계 TFU을 통해 최소화 된 AgNPs을 집중합니다. 대표 샘플은 전송 전자 현미경, UV-VIS 흡수 분광 광도법, 라만 분광법 및 유도 결합 플라즈마 광 방출 분광법을 사용하여 특징되었습니다. 최종 retentate이 높은 농축 (4 ML, 8,539.9 μg ML -1) 아직 통합과 균일 겸손한으로 구성직경 1-20 nm의 AgNPs. 이 62 % 정도의 실버 농도 수율에 해당합니다.

Watch this Scientific Journal Video about Tangential Flow Ultrafiltration: A  Ã¢â‚¬Å“GreenÃ¢â‚¬Â Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles at JoVE.com

Tangential Flow Ultrafiltration: A  ÃƒÂ¢Ã¢â€šÂ¬Ã…â€œGreenÃƒÂ¢Ã¢â€šÂ¬Ã‚Â Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles

접선 흐름 ultrafiltration (TFU)는 biosamples의 무게 기반의 분리에 사용되는 재순환 방식입니다. TFU는 크기를 선택합니다 (1-20 nm 정도의 직경)에 적응하고 높은 polydisperse 실버 나노 입자의 큰 볼륨 (15.2 μg의 ML 4 L을 집중했다<sup&gt; -1</sup8,539.9 μg의 ML의&gt; 4 다운 ML<sup&gt; -1</sup&gt;) 최소한의 집합이 있습니다.

접선 흐름 Ultrafiltration : 콜로이드 실버 나노 입자의 크기 선택과 집중을위한 &quot;그린&quot;방법

Nowadays, AgNPs are extensively used in the manufacture of consumer products,1 water disinfectants,2 therapeutics,1, 3 and biomedical devices4 due to ...

Research

JoVE Journal

Biology

Tangential Flow Ultrafiltration: A  &ldquo;Green&rdquo; Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles

A Method for 2-Photon Imaging of Blood Flow in the Neocortex through a Cranial Window

두개골의 창문을 통해 네크로 텍스의 혈류의 2 광자 이미징을위한 방법

The ability to image the cerebral vasculature (from large vessels to capillaries) and record blood flow dynamics in the intact brain of living rodents is ...

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생물학

Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat

쥐에서 도보의 질적 분석 방법으로 Rotorod의 사용

High speed videoanalysis of the details of movement can provide a source of information about qualitative aspects of walking movements. When walking on a ...

A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients

안정적인 농도 기울기에 박테리아 Chemotaxis을 Quantifying위한 Microfluidic 장치

Chemotaxis allows bacteria to approach sources of attractant chemicals or to avoid sources of repellent chemicals. Bacteria constantly monitor the ...

Analysis of the Development of a Morphological Phenotype as a Function of Protein Concentration in Budding Yeast

버딩 효모에서 단백질 농도의 함수로 형태학의 표현형의 발전 분석

Gene deletion and protein overexpression are common methods for studying functions of proteins. In this article, we describe a protocol for analysis of ...

Flow Cytometry Purification of Mouse Meiotic Cells

마우스 Meiotic 셀 유동세포계측법의 정제

The heterogeneous nature of cell types in the testis and the absence of meiotic cell culture models have been significant hurdles to the study of the ...

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes

셀 추적 염료를 사용하여 전지 부문 모니터링에 최적화 된 착색 및 확산 모델링 방법

Fluorescent cell tracking dyes, in combination with flow and image cytometry, are powerful tools with which to study the interactions and fates of ...

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

재조합 단백질의 미끼를 사용 파지 디스플레이와 궁합 선택을위한 프로토콜

Using  recombinant phage as a scaffold to present various protein portions encoded by  a directionally cloned cDNA library to immobilized bait molecules ...

An Innovative Method for Exosome Quantification and Size Measurement

엑소 정량화 및 크기 측정을위한 혁신적인 방법

Although the biological importance of exosomes has recently gained an increasing amount of scientific and clinical attention, much is still unknown about ...

Development of a Colloidal Gold-based Immunochromatographic Test Strip for Detection of Cetacean Myoglobin

고래 마이오글로빈의 검출을위한 콜로이드 골드 기반 면역 크로마토 그래피 테스트 스트립의 개발

This protocol describes the development of a colloidal gold immunochromatographic test strip based on the sandwich format that can be used to ...

Filtration Isolation of Nucleic Acids:  A Simple and Rapid DNA Extraction Method

핵산의 여과 분리 : 간단하고 신속한 DNA 추출 방법

FINA, filtration isolation of nucleic acids, is a novel extraction method which utilizes vertical filtration via a separation membrane and absorbent pad ...