Финансирование от Национального научного фонда через Нюрнберг в технике и программы ЛИДЕР консорциума с благодарностью.

1. Синтез коллоидных AgNPs Механизм реакции для метода Крейтон (слегка измененный, недорогой) 22 описан очень подробно в поддержку информации ссылка Павел et.al вместе с нежелательный гидролиз побочных реакции NaBH 4 при комнатной температуре или выше 23. <ol><li> ?...

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UV-Vis абсорбционной спектрофотометрии и спектроскопии комбинационного рассеяния коллоидных AgNPs Хорошо известно, что число поверхностных плазмонов резонансных пиков в спектре поглощения коллоидных уменьшается с симметрией AgNPs увеличивается. Кроме того, ССПС агрегации пр...

<table border="1"><tr><td> Нитрат серебра (AgNO 3) </td><td> Acros Organics Инк </td><td> CAS: 7761-88-8 </td><td></td></tr><tr><td> Боргидрид натрия (NaBH 4) </td><td> Acros Organics Инк </td><td> CAS: 16940-66-2 </td><td></td></tr><tr><td> Азотная кислота (HNO 3, Optima) </td><td> Fisher Scientific Инк </td><td> A467-1 </td><td> Трассировка металла класса для МСП анализа </td></tr><tr><td> 10000 мкг мл -1 серебряный стандарт, EnviroConcentrate </td><td> Ультра Научные </td><td> Американо-IAA-047 </td><td></td></tr><tr><td> KrosFlo исследований II я Тангенциальная система фильтрации потока </td><td> Spectrum Laboratories, Inc </td><td> <a href="http://www.spectrumlabs.com/pshop/RequestPrice/index.html?LiFrom=%2Ffiltration%2FKR2SystemO.html;FrName=Research+IIi+System;Pn=SYR2-U20-01N;" target="_blank">SYR2-U20-01N</a> </td><td></td></tr><tr><td> PS 0,05 мкм (0,5 мм) 460 см 2 </td><td> Spectrum Laboratories, Inc </td><td> <a href="http://www.spectrumlabs.com/filtration/MidiKros.html?Pn=X30S-900-02N;" target="_blank">X30S-900-02N</a> </td><td></td></tr><tr><td> Midi 100 кДа PS 200 см 2 </td><td> Spectrum Laboratories, Inc </td><td> X3-100S-901-02N </td><td></td></tr><tr><td> Micro100 кДа PS 20 см 2 </td><td> Spectrum Laboratories, Inc </td><td> X1AB-300-10N </td><td></td></tr><tr><td> MasterFlex C-Flex трубка L / S Размер 17 </td><td> Cole-Palmer машиностроительный завод </td><td> 06424-17 </td><td></td></tr><tr><td> MasterFlex C-Flex трубка L / S Размер 14 </td><td> Cole-Palmer машиностроительный завод </td><td> 06424-14 </td><td></td></tr><tr><td> Cary 50 UV-VIS-NIR спектрофотометр </td><td> Varian Инк </td><td></td><td></td></tr><tr><td> LabRam HR 800 системы </td><td> Horiba Jobin Yvon Инк </td><td></td><td></td></tr><tr><td> Varian 710ES ICP-OES </td><td> Varian Инк </td><td></td><td></td></tr></table> Таблица 1. Специфических реагентов и оборудования.

tangential flow ultrafiltration: a  &ldquo;green&rdquo; method for the size selection and concentration of colloidal silver nanoparticles

Nowadays, AgNPs are extensively used in the manufacture of consumer products,1 water disinfectants,2 therapeutics,1, 3 and biomedical devices4 due to their powerful antimicrobial properties.3-6 These nanoparticle applications are strongly influenced by the AgNP size and aggregation state. Many challenges exist in the controlled fabrication7 and size-based isolation4,8 of unfunctionalized, homogenous AgNPs that are free from chemically aggressive capping/stabilizing agents or organic solvents.7-13 Limitations emerge from the toxicity of reagents, high costs or reduced efficiency of the AgNP synthesis or isolation methods (e.g., centrifugation, size-dependent solubility, size-exclusion chromatography, etc.).10,14-18 To overcome this, we recently showed that TFU permits greater control over the size, concentration and aggregation state of Creighton AgNPs (300 ml of 15.3 &mu;g ml-1 down to 10 ml of 198.7 &mu;g ml-1) than conventional methods of isolation such as ultracentrifugation.19
TFU is a recirculation method commonly used for the weight-based isolation of proteins, viruses and cells.20,21 Briefly, the liquid sample is passed through a series of hollow fiber membranes with pore size ranging from 1,000 kD to 10 kD. Smaller suspended or dissolved constituents in the sample will pass through the porous barrier together with the solvent (filtrate), while the larger constituents are retained (retentate). TFU may be considered a "green" method as it neither damages the sample nor requires additional solvent to eliminate toxic excess reagents and byproducts. Furthermore, TFU may be applied to a large variety of nanoparticles as both hydrophobic and hydrophilic filters are available.
The two main objectives of this study were: 1) to illustrate the experimental aspects of the TFU approach through an invited video experience and 2) to demonstrate the feasibility of the TFU method for larger volumes of colloidal nanoparticles and smaller volumes of retentate. First, unfuctionalized AgNPs (4 L, 15.2 &mu;g ml-1) were synthesized using the well-established Creighton method22,23 by the reduction of AgNO3 with NaBH4. AgNP polydispersity was then minimized via a 3-step TFU using a 50-nm filter (460 cm2) to remove AgNPs and AgNP-aggregates larger than 50 nm, followed by two 100-kD (200 cm2 and 20 cm2) filters to concentrate the AgNPs. Representative samples were characterized using transmission electron microscopy, UV-Vis absorption spectrophotometry, Raman spectroscopy, and inductively coupled plasma optical emission spectroscopy. The final retentate consisted of highly concentrated (4 ml, 8,539.9 &mu;g ml-1) yet lowly aggregated and homogeneous AgNPs of 1-20 nm in diameter. This corresponds to a silver concentration yield of about 62%.

Watch this Scientific Journal Video about Tangential Flow Ultrafiltration: A  Ã¢â‚¬Å“GreenÃ¢â‚¬Â Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles at JoVE.com

Tangential Flow Ultrafiltration: A  ÃƒÂ¢Ã¢â€šÂ¬Ã…â€œGreenÃƒÂ¢Ã¢â€šÂ¬Ã‚Â Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles

Тангенциальный ультрафильтрации потока (ФПУ) является рециркуляция метод, используемый для веса на основе разделения биообъектов. TFU был адаптирован к размеру выберите (1-20 нм в диаметре) и очень сконцентрировать большое количество полидисперсных наночастиц серебра (4 л на 15,2 мкг мл<sup&gt; -1</sup&gt; До 4 мл 8,539.9 мкг мл<sup&gt; -1</sup&gt;) С минимальным агрегации.

Тангенциальный ультрафильтрации потока: &quot;Зеленый&quot; способ выбора размера и концентрации коллоидных наночастиц серебра

Nowadays, AgNPs are extensively used in the manufacture of consumer products,1 water disinfectants,2 therapeutics,1, 3 and biomedical devices4 due to ...

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Biology

Tangential Flow Ultrafiltration: A  &ldquo;Green&rdquo; Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles

A Method for 2-Photon Imaging of Blood Flow in the Neocortex through a Cranial Window

Метод 2-Фотон изображений кровотока в коре головного мозга через черепной Window

The ability to image the cerebral vasculature (from large vessels to capillaries) and record blood flow dynamics in the intact brain of living rodents is ...

Use of Rotorod as a Method for the Qualitative Analysis of Walking in Rat

Использование Rotorod как метод Качественный анализ Прогулка по Крыса

High speed videoanalysis of the details of movement can provide a source of information about qualitative aspects of walking movements. When walking on a ...

A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients

Микрожидкостных Устройство для Количественная Бактериальные Хемотаксис в стабильных градиента концентрации

Chemotaxis allows bacteria to approach sources of attractant chemicals or to avoid sources of repellent chemicals. Bacteria constantly monitor the ...

Analysis of the Development of a Morphological Phenotype as a Function of Protein Concentration in Budding Yeast

Анализ развития Морфологические Фенотип как функция концентрации белка в почкующихся дрожжей

Gene deletion and protein overexpression are common methods for studying functions of proteins. In this article, we describe a protocol for analysis of ...

Flow Cytometry Purification of Mouse Meiotic Cells

Проточной цитометрии Очистка мыши мейоза клетки

The heterogeneous nature of cell types in the testis and the absence of meiotic cell culture models have been significant hurdles to the study of the ...

Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes

Оптимизирован Окрашивание и распространения методов моделирования для деления клеток Мониторинг использования красителей сотовых слежения

Fluorescent cell tracking dyes, in combination with flow and image cytometry, are powerful tools with which to study the interactions and fates of ...

A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits

Протокол для фагов и Affinity выделение с помощью рекомбинантного белка Приманки

Using  recombinant phage as a scaffold to present various protein portions encoded by  a directionally cloned cDNA library to immobilized bait molecules ...

An Innovative Method for Exosome Quantification and Size Measurement

Инновационный метод для экзосома количественной и измерения размера

Although the biological importance of exosomes has recently gained an increasing amount of scientific and clinical attention, much is still unknown about ...

Development of a Colloidal Gold-based Immunochromatographic Test Strip for Detection of Cetacean Myoglobin

Разработка коллоидного золота на основе Иммунохроматографические тест-полоски для обнаружения китообразных миоглобина

This protocol describes the development of a colloidal gold immunochromatographic test strip based on the sandwich format that can be used to ...

Filtration Isolation of Nucleic Acids:  A Simple and Rapid DNA Extraction Method

Фильтрация Выделение нуклеиновых кислот: Простой и быстрый ДНК Метод экстракции

FINA, filtration isolation of nucleic acids, is a novel extraction method which utilizes vertical filtration via a separation membrane and absorbent pad ...