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1. Kolloidal AGNPS sentezi Creighton yöntemi (biraz değiştirilmiş ucuz) 22 için reaksiyon mekanizması istenmeyen hidroliz, oda sıcaklığında ya da daha yüksek NaBH4 yan reaksiyon ile birlikte referans Pavel et.al arasında desteklenmesi bilgi de ayrıntılı olarak tarif edilmektedir. 23 <ol><li> 4-12% 40 etanol banyosu içinde bir 1.25 M NaOH içinde, hr ve son olarak otoklav, daha sonra da% 10 HNO 3 banyosu içinde 12-24 saat için he...

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UV-Vis Absorbsiyon Spektrometresi ve Kolloidal AGNPS Raman Spektroskopisi Bu, iyi bir kolloid absorbsiyon spektrumunu, yüzey plazmon rezonans tepe sayı AGNPS artar simetri olarak azalttığı bilinmektedir. Ayrıca, daha geniş veya daha AgNP toplama kırmızı-kaydırılmış zirveleri görünüme neden olur. 25,26 394 nm &#39;de bir tek, keskin ve simetrik SPR tepe varlığı orta toplama ve boyut dağılımının küçük, yuvarlak AGNPS göstergesidir. <p class="jove_conten...

<table border="1"><tr><td> Gümüş nitrat (AgNO 3) </td><td> Acros Organics Inc </td><td> CAS: 7761-88-8 </td><td></td></tr><tr><td> Sodyum borohidrid (NaBH4) </td><td> Acros Organics Inc </td><td> CAS: 16940-66-2 </td><td></td></tr><tr><td> Nitrik asit (HNO 3, Optima) </td><td> Fisher Scientific Inc </td><td> A467-1 </td><td> ICP analizi için metal tenörü Trace </td></tr><tr><td> 10.000 mg ml -1 gümüş standart, EnviroConcentrate </td><td> Ultra Bilimsel </td><td> ABD-IAA-047 </td><td></td></tr><tr><td> KrosFlo Araştırması II i Teğet Akış Filtrasyon Sistemi </td><td> Spektrum Laboratories Inc </td><td> <a href="http://www.spectrumlabs.com/pshop/RequestPrice/index.html?LiFrom=%2Ffiltration%2FKR2SystemO.html;FrName=Research+IIi+System;Pn=SYR2-U20-01N;" target="_blank">SYR2-U20-01N</a> </td><td></td></tr><tr><td> 0.05 mikron PS (0.5 mm) 460 cm 2 </td><td> Spektrum Laboratories Inc </td><td> <a href="http://www.spectrumlabs.com/filtration/MidiKros.html?Pn=X30S-900-02N;" target="_blank">X30S-900-02N</a> </td><td></td></tr><tr><td> Midi 100 kD PS 200 cm 2 </td><td> Spektrum Laboratories Inc </td><td> X3-100S-901-02N </td><td></td></tr><tr><td> Micro100 kD PS 20 cm 2 </td><td> Spektrum Laboratories Inc </td><td> X1AB-300-10N </td><td></td></tr><tr><td> MASTERFLEX C-Flex hortum L / S Boyutu 17 </td><td> Cole-Palmer Instrument Co </td><td> 06424-17 </td><td></td></tr><tr><td> MASTERFLEX C-Flex hortum L / S Boyutu 14 </td><td> Cole-Palmer Instrument Co </td><td> 06424-14 </td><td></td></tr><tr><td> Cary 50 UV-VIS-NIR spektrofotometre </td><td> Varian Inc </td><td></td><td></td></tr><tr><td> LabRam HR 800 sistemi </td><td> Horiba Jobin Yvon A.Ş. </td><td></td><td></td></tr><tr><td> Varian 710ES ICP-OES </td><td> Varian Inc </td><td></td><td></td></tr></table> Tablo 1. Özgül reaktifler ve ekipman.

tangential flow ultrafiltration: a  &ldquo;green&rdquo; method for the size selection and concentration of colloidal silver nanoparticles

Günümüzde yaygın olarak bunların AGNPS güçlü antimikrobiyel özellikleri nedeniyle tüketici ürünleri, 1 su dezenfektanlar, 2 Therapeutics, 1, 3 ve 4 Biyomedikal cihazların üretiminde kullanılmaktadır. 3-6 Bu nanoparçacık uygulamalar kuvvetle AgNP boyut ve toplama durum tarafından etkilenmektedir . Birçok zorluklar kontrollü imalat 7 ve kapaklama / ajanlar veya organik çözücüler stabilize agresif kimyasal arınmış unfunctionalized, homojen AGNPS büyüklüğü tabanlı yalıtım 4,8 var. 7-13 Sınırlamalar, reaktiflerin toksisitesi yüksek maliyetler ortaya veya azaltılmış AgNP sentez veya izolasyon yöntemleri (örneğin, santrifüj, boyut bağımlı çözünürlük, boyut dışlama kromatografisi vb) verimlilik. 10,14-18 Bunun üstesinden gelmek için, son zamanlarda TFU büyükse, konsantrasyonu üzerinde kontrol ve izin gösterdi Creighton AGNPS (300 agregasyonu devletBöyle Ultrasantrifügasyon gibi izolasyon geleneksel yöntemlere kıyasla 198.7 mg ml -1) 15.3 mg ml -1 ml aşağı 10 ml. 19 TFU genellikle proteinler, virüslerin ve hücrelerin ağırlık temelli izolasyon için kullanılan bir devridaim yöntemi. Kısaca 20,21, sıvı numune kD 1.000 ila 10 kD arasında değişen gözenek büyüklüğüne sahip içi boş elyaf membran bir dizi geçirilir. Büyük bileşenlerin (retentat) aynen korunurken örnek küçük askıya veya çözünmüş bileşenler (süzüntü) çözücü ile birlikte gözenekli bariyer geçecek. TFU o ne zarar örnek olarak &quot;yeşil&quot; bir yöntem olarak kabul veya zehirli aşırı ayıraç ve yan ortadan kaldırmak için ek çözücü gerektirir olabilir. Her ikisi de hidrofobik ve hidrofilik filtreler bulunmaktadır Dahası, TFU nanoparçacıklar büyük bir çeşitlilik için uygulanabilir. Bu çalışmanın iki temel amacı vardı: 1) göstermek içinBir davet video deneyimi ve 2 ile TFU yaklaşımın deneysel yönlerini) kolloidal nanopartiküller ve retentat daha küçük hacimli büyük hacimler için TFU yöntemin uygulanabilirliğini göstermek için. İlk olarak, unfuctionalized AGNPS (4 L, 15.2 ug ml-1) NaBH4 ile AgNO 3 azaltılması ile iyi kurulmuş Creighton 22,23 yöntem kullanılarak sentezlendi. AgNP polidispersite sonra iki 100-kD (200 cm 2 ve 20 cm 2) Filtreleri takip AGNPS ve AgNP-agrega daha büyük 50 nm, kaldırmak için bir 50-nm filtresi (460 cm 2) kullanılarak bir 3-adım TFU yoluyla minimize edildi AGNPS konsantre. Örnek örnekleri transmisyon elektron mikroskobu, UV-Vis absorpsiyon spektrofotometresi, Raman spektroskopisi ve indüktif eşleşmiş plazma optik emisyon spektroskopisi ile karakterize edildi. Nihai retentat yüksek konsantrasyonlu (4 mi, 8,539.9 mg ml-1) henüz birleştirilmiş ve homojen aşağı oluşuyorduÇapı 1-20 nm AGNPS. Bu, yaklaşık% 62 arasında bir konsantrasyonda gümüş verim karşılık gelir.

Watch this Scientific Journal Video about Tangential Flow Ultrafiltration: A  Ã¢â‚¬Å“GreenÃ¢â‚¬Â Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles at JoVE.com

Tangential Flow Ultrafiltration: A  ÃƒÂ¢Ã¢â€šÂ¬Ã…â€œGreenÃƒÂ¢Ã¢â€šÂ¬Ã‚Â Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles

Teğet Akış ultrafiltrasyon (TFU) biosamples ağırlığı bazlı ayrılması için kullanılan bir devridaim yöntemi. TFU boyutu-select (1-20 nm çapında) uyarlanmış ve yüksek polidispers gümüş nanopartikülleri büyük bir hacmi (15.2 mikrogram ml 4 L konsantre edildi<sup&gt; -1</sup8,539.9 mikrogram ml&gt; 4 aşağı ml<sup&gt; -1</sup&gt;) Minimal agregasyonu ile.

Teğet Akış Ultrafiltrasyon: Kolloidal Gümüş Nanopartiküllerin Boyutu Seçme ve Konsantrasyon için &quot;Yeşil&quot; Yöntemi

Nowadays, AgNPs are extensively used in the manufacture of consumer products,1 water disinfectants,2 therapeutics,1, 3 and biomedical devices4 due to ...

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Tangential Flow Ultrafiltration: A  &ldquo;Green&rdquo; Method for the Size Selection and Concentration of Colloidal Silver Nanoparticles

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