Name: postproduction processing of electrospun fibres for tissue engineering
Uploaded: 2012-08-09T13:00:00
Description: Watch this Scientific Journal Video about Postproduction Processing of Electrospun Fibres for Tissue Engineering at JoVE.com

Vi takker BBSRC for at finansiere en ph.d. for Mr. Frazer Bye.

1. Electrospinning af tilfældige og Aligned Fibre Electrospinning skaber fine fiber-netværk ved hjælp af elektriske potentiale til at trække en polymer løsning på vej mod en jordet kollektor. Samlere kan være i mange former og kan være statisk eller, mere almindeligt, at rotere. Opløsningsmidlet fordamper, før opløsningen ankommer ved kollektoren, og strålen størkner til en fiber. Hver polymer kræver sit eget sæt af betingelser til frembringelse af e...

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	<li>Canton, I., McKean, R., Charnley, M., Blackwood, K., Fiorica, C., Ryan, A., MacNeil, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+an+Ibuprofen-releasing+biodegradable+PLA/PGA+electrospun+scaffold+for+tissue+regeneration.">Development of an Ibuprofen-releasing biodegradable PLA/PGA electrospun scaffold for tissue regeneration.</a> Biotechnology and bioengineering. 105, 396-408 (2010).</li><li>Blackwood, K., McKean, R., Canton, I., Freeman, C., Franklin, K., Cole, A., Brook, I., Farthing, P., Rimmer, S., Haycock, J., Ryan, A., MacNeil, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+biodegradable+electrospun+scaffolds+for+dermal+replacement.">Development of biodegradable electrospun scaffolds for dermal replacement.</a> Biomaterials. 29, 3091-3104 (2008).</li><li>Yang, F., Maurugan, R., Wang, S., Ramakrishna, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electrospinning+of+nano/micro+scale+poly(L-lactic+acid)+aligned+fibers+and+their+potential+in+neural+tissue+engineering.">Electrospinning of nano/micro scale poly(L-lactic acid) aligned fibers and their potential in neural tissue engineering.</a> Biomaterials. 26, 2603-2610 (2005).</li><li>Sittichokechaiwut, A., Edwards, J. H., Scutt, A. M., Reilly, G. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Short+bouts+of+mechanical+loading+are+as+effective+as+dexamethasone+at+inducing+matrix+production+by+human+bone+marrow+mesenchymal+stem+cell.">Short bouts of mechanical loading are as effective as dexamethasone at inducing matrix production by human bone marrow mesenchymal stem cell.</a> Eur. Cell Mater. 20, 45-57 (2010).</li><li>Sill, T. J., von Recum, H. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electrospinning:+applications+in+drug+delivery+and+tissue+engineering.">Electrospinning: applications in drug delivery and tissue engineering.</a> Biomaterials. 29 (13), 1989-2006 (2008).</li><li>Deitzel, J., Kleinmeyer, J., Harris, D., Beck Tan, N. C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+effect+of+processing+variables+on+the+morphology+of+electrospun+nanofibers+and+textiles.">The effect of processing variables on the morphology of electrospun nanofibers and textiles.</a> Polymer. 42, 261-272 (2001).</li><li>Fridrikh, S., Yu, J., Brenner, M., Rutledge, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Controlling+the+fiber+diameter+during+electrospinning.">Controlling the fiber diameter during electrospinning.</a> Physical review letters. 90, 1-4 (2003).</li><li>Fong, H., Chun, I., Reneker, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Beaded+nanofibers+formed+during+electrospinning.">Beaded nanofibers formed during electrospinning.</a> Polymer. 40 (16), 4585-4592 (1999).</li><li>Selim, M., Bullock, A. J., Blackwood, K. A., Chapple, C. R., MacNeil, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Developing+biodegradable+scaffolds+for+tissue+engineering+of+the+urethra.">Developing biodegradable scaffolds for tissue engineering of the urethra.</a> BJU Int. 107 (2), 296-302 (2010).</li><li>Tong, H. -. W., Wang, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+investigation+into+the+influence+of+electrospinning+parameters+on+the+diameter+and+alignment+of+poly(hydroxybutyrate-co-hydroxyvalerate)+fibers.">An investigation into the influence of electrospinning parameters on the diameter and alignment of poly(hydroxybutyrate-co-hydroxyvalerate) fibers.</a> Journal of Applied Polymer Science. 120 (3), 1694-1706 (2011).</li><li>Tong, H. -. W., Wang, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Electrospinning+of+poly(hydroxybutyrate-co-hydroxyvalerate)+fibrous+tissue+engineering+scaffolds+in+two+different+electric+fields.">Electrospinning of poly(hydroxybutyrate-co-hydroxyvalerate) fibrous tissue engineering scaffolds in two different electric fields.</a> Polymer Engineering &amp; Science. 51 (7), 1325-1338 (2011).</li><li>Retzepi, M., Donos, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Guided+Bone+Regeneration:+biological+principle+and+therapeutic+applications.">Guided Bone Regeneration: biological principle and therapeutic applications.</a> Clinical oral implants research. 21, 567-576 (2010).</li><li>Moreau, J., Caccamese, J., Coletti, D., Sauk, J., Fisher, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tissue+engineering+solutions+for+cleft+palates.">Tissue engineering solutions for cleft palates.</a> Journal of oral maxillofacial. 65, 2503-2511 (2007).</li><li>Yang, F., Both, S., Yang, X., Walboomers, X., Jansen, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Development+of+an+electrospun+nano-apatite/PCL+composite+membrane+for+GTR/GBR+application.">Development of an electrospun nano-apatite/PCL composite membrane for GTR/GBR application.</a> Acta biomaterialia. 5, 3295-3304 (2009).</li><li>Yoshimoto, H., Shin, Y., Terai, H., Vacanti, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+biodegradable+nanofiber+scaffold+by+electrospinning+and+its+potential+for+bone+tissue+engineering.">A biodegradable nanofiber scaffold by electrospinning and its potential for bone tissue engineering.</a> Biomaterials. 24, 2077-2082 (2003).</li><li>Telemeco, T., Ayres, C., Bowlin, G., Wnek, G., Boland, E., Cohen, N., Baumgarten, C., Mathews, J., Simpson, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regulation+of+cellular+infiltration+into+tissue+engineering+scaffolds+composed+of+submicron+diameter+fibrils+produced+by+electrospinning.">Regulation of cellular infiltration into tissue engineering scaffolds composed of submicron diameter fibrils produced by electrospinning.</a> Acta biomaterialia. 1, 377-385 (2005).</li></ol>

Electrospinning er en meget populær teknik til fremstilling af stilladser til vævsmanipulering. 14, 15, 16 Selv om det er forholdsvis simpelt at fremstille basale electrospun scaffolds til eksperimentel anvendelse teknikken er også kompleks og multifacetteret med mange variable. 6 Der er mange undersøgelser, der beskriver, hvorledes electrospinning parametre bestemmer stilladset fremstillet. I denne undersøgelse er fokus på de betydelige udfordringer, post-produktion at gøre stilladser passe...

<table border="1"><tbody><tr><td> Navn på reagenset </td><td> Firma </td><td> Katalog nummer </td><td> Kommentarer </td></tr><tr><td> Poly mælke-co-glycolsyre </td><td> Sigma Aldrich </td><td></td><td></td></tr><tr><td> Polymælkesyre </td><td> Sigma Aldrich </td><td> 81273 </td><td> Iboende viskositet ~ 2.0dl / g </td></tr><tr><td> Poly ε-caprolacton </td><td> Sigma Aldrich </td><td></td><td></td></tr><tr><td> Poly hydroxybutyrat-co-hydroxyvaleratenheder 00:01 </td><td> Goodfellow </td><td> 578-446-59 </td><td> PHB88/PHV12 </td></tr><tr><td> Dichlormethan </td><td> Sigma Aldrich eller Fisher </td><td> 270997 eller D/1850/17 </td><td> &gt; 99,8% indeholder 50 150 ppm amylen stabilisator </td></tr><tr><td> 50 multi farvede balloner </td><td> Wilkinson hardware Stores Ltd </td><td> 0105790 </td><td></td></tr><tr><td> Gede-anti-kanin-IgG (Fc): FITC </td><td> AbDserotec </td><td> STAR121F </td><td></td></tr><tr><td> Kanin-anti-humant alfa elastin </td><td> AbDserotec </td><td> 4060-1060 </td><td></td></tr><tr><td> Screw Cap GL45 PP 2 Port, PK / 2 </td><td> SLS </td><td> 1129750 </td><td></td></tr><tr><td> 4 &#39;,6-diamidino-2-phenylindol-dihydrochlorid </td><td> Sigma Aldrich </td><td> 32670 </td><td></td></tr><tr><td> CellTracker grøn CMFDA </td><td> Invitrogen </td><td> C7025 </td><td></td></tr><tr><td> CellTracker rød CMTX </td><td> Invitrogen </td><td> C34552 </td><td></td></tr></tbody></table>

postproduction processing of electrospun fibres for tissue engineering

Electrospinning er en almindeligt anvendt og alsidig fremgangsmåde til fremstilling af stilladser, ofte bionedbrydelige) til 3D vævsmanipulering. 1, 2, 3 Mange væv in vivo underkastes biaksial udspiling i forskelligt omfang, såsom hud, blære, bækkenbunden og selv den hårde gane som børn vokse. At producere stilladser til disse formål er der behov for at udvikle scaffolds passende biomekaniske egenskaber (enten opnås med eller uden celler) og som er sterile til klinisk anvendelse. Fokus i dette papir er ikke sådan til at etablere grundlæggende electrospinning parametre (da der er omfattende litteratur om electrospinning), men på hvordan man kan ændre spundet stilladser post produktion for at gøre dem egnede til vævsopbygning formål - her tykkelse, mekaniske egenskaber og sterilisering (kræves til klinisk brug) anses for at være, og vi også beskrive, hvordan cellerne kan dyrkes på stilladser og udsat for biaksial belastning at betingelse dem til specifikke applikationer. Electrospinning tendens til at producere tynde plader, som electrospinning kollektoren bliver belagt med isolerende fibre bliver en dårlig leder, således at fibrene ikke længere aflejring på den. Derfor beskriver vi fremgangsmåder til at fremstille tykkere strukturer ved hjælp af varme eller damp annealing øge styrken af ​​stilladser, men ikke nødvendigvis elasticitet. Sekventielle spinding af stilladser af forskellige polymerer for at opnå komplekse scaffolds er også beskrevet. Sterilisering metoder kan påvirke styrke og elasticitet af stilladser. Vi sammenligner tre metoder til deres virkninger på de biomekaniske egenskaber på electrospun scaffolds poly mælkesyre-co-glycolsyre (PLGA). Billeddannelse af celler på stilladserne og vurdering af produktionen af ​​ekstracellulære matrix (ECM)-proteiner fra celler på stilladserne er beskrevet. Dyrkning af celler på stilladserne in vitro kan forbedre stillads styrke og elasticitet, men vævsmanipulering literature viser, at celler ofte ikke at frembringe passende ECM når den dyrkes under statiske betingelser. Der er få kommercielle systemer tilgængelige, som tillader en at dyrke celler på stilladserne under dynamiske conditioning regimer. - Et eksempel er Bose ElectroForce 3100, som kan anvendes til at udøve en konditionering program på celler i stilladser holdt ved anvendelse af mekaniske styr inden for et medie fyldt kammer 4 En tilgang til et budget cellekultur bioreaktor for kontrolleret forvrængning i 2 dimensioner er beskrevet. Vi viser, at celler kan induceres til at producere elastin under disse betingelser. Endelig vurdering af biomekaniske egenskaber af forarbejdede scaffolds dyrket med eller uden celler er beskrevet.

Watch this Scientific Journal Video about Postproduction Processing of Electrospun Fibres for Tissue Engineering at JoVE.com

Postproduction Processing of Electrospun Fibres for Tissue Engineering

Electrospun stilladser kan bearbejdes post produktion til vævsdyrkningsapplikationer. Her beskriver vi metoder til spinding komplekse stilladser (af på hinanden følgende spinding), for at gøre tykkere stilladser (ved multi-lag ved hjælp af varme eller damp udglødning), for at opnå sterilitet (aseptisk produktion eller sterilisering post produktion), og for at opnå passende biomekaniske egenskaber.

Postproduktion Behandling af Electrospun Fibre til Tissue Engineering

Electrospinning is a commonly used and versatile method to produce  scaffolds (often biodegradable) for 3D tissue engineering.1, 2, 3 Many tissues in vivo ...

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