Name: postproduction processing of electrospun fibres for tissue engineering
Uploaded: 2012-08-09T13:00:00
Description: Watch this Scientific Journal Video about Postproduction Processing of Electrospun Fibres for Tissue Engineering at JoVE.com

हम श्री फ्रेज़र अलविदा के लिए एक पीएचडी वित्त पोषण के लिए बीबीएसआरसी धन्यवाद.

1. रैंडम और निरपेक्ष रेशे की electrospinning Electrospinning बिजली क्षमता का उपयोग करने के लिए एक भूसम्पर्कित कलेक्टर की ओर एक बहुलक समाधान आकर्षित द्वारा ठीक रेशेदार नेटवर्क बनाता है. कलेक्टरों बहुत कई आकार मे?...

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Electrospinning ऊतक इंजीनियरिंग के लिए scaffolds के उत्पादन के लिए एक बहुत लोकप्रिय तकनीक है 14, 16, 15. हालांकि यह अपेक्षाकृत सरल है प्रयोगात्मक उपयोग के लिए बुनियादी electrospun scaffolds के उत्पादन तकनीक भी कई चर के साथ जटिल और बह?...

<table border="1"><tbody><tr><td> अभिकर्मक का नाम </td><td> कंपनी </td><td> सूचीपत्र संख्या </td><td> टिप्पणियाँ </td></tr><tr><td> पाली लैक्टिक - सह - glycolic एसिड </td><td> सिग्मा Aldrich </td><td></td><td></td></tr><tr><td> पाली लैक्टिक एसिड </td><td> सिग्मा Aldrich </td><td> ८१,२७३ </td><td> निहित चिपचिपापन ~ 2.0dl / छ </td></tr><tr><td> पाली ε - caprolactone </td><td> सिग्मा Aldrich </td><td></td><td></td></tr><tr><td> पाली hydroxybutyrate सह hydroxyvalerate 00:01 </td><td> गूड </td><td> 578-446-59 </td><td> PHB88/PHV12 </td></tr><tr><td> Dichloromethane </td><td> सिग्मा Aldrich या फिशर </td><td> 270,997 या D/1850/17 </td><td> &gt; 99.8% 50-150ppm amylene स्टेबलाइजर शामिल हैं </td></tr><tr><td> 50 बहु रंगीन गुब्बारे </td><td> विल्किनसन हार्डवेयर स्टोर लिमिटेड </td><td> 0105790 </td><td></td></tr><tr><td> बकरी विरोधी खरगोश आईजीजी: FITC (एफसी) </td><td> AbDserotec </td><td> STAR121F </td><td></td></tr><tr><td> खरगोश विरोधी मानव अल्फा elastin </td><td> AbDserotec </td><td> 4060-1060 </td><td></td></tr><tr><td> भाड़ GL45 पीपी 2 पोर्ट कैप, पी / 2 </td><td> SLS </td><td> 1129750 </td><td></td></tr><tr><td> 4 &#39;dihydrochloride ,6-Diamidino-2-phenylindole </td><td> सिग्मा Aldrich </td><td> 32,670 </td><td></td></tr><tr><td> CellTracker हरे रंग CMFDA </td><td> Invitrogen </td><td> C7025 </td><td></td></tr><tr><td> CellTracker लाल CMTX </td><td> Invitrogen </td><td> C34552 </td><td></td></tr></tbody></table>

postproduction processing of electrospun fibres for tissue engineering

Electrospinning is a commonly used and versatile method to produce scaffolds (often biodegradable) for 3D tissue engineering.1, 2, 3 Many tissues in vivo undergo biaxial distension to varying extents such as skin, bladder, pelvic floor and even the hard palate as children grow. In producing scaffolds for these purposes there is a need to develop scaffolds of appropriate biomechanical properties (whether achieved without or with cells) and which are sterile for clinical use. The focus of this paper is not how to establish basic electrospinning parameters (as there is extensive literature on electrospinning) but on how to modify spun scaffolds post production to make them fit for tissue engineering purposes - here thickness, mechanical properties and sterilisation (required for clinical use) are considered and we also describe how cells can be cultured on scaffolds and subjected to biaxial strain to condition them for specific applications.
Electrospinning tends to produce thin sheets; as the electrospinning collector becomes coated with insulating fibres it becomes a poor conductor such that fibres no longer deposit on it. Hence we describe approaches to produce thicker structures by heat or vapour annealing increasing the strength of scaffolds but not necessarily the elasticity. Sequential spinning of scaffolds of different polymers to achieve complex scaffolds is also described. Sterilisation methodologies can adversely affect strength and elasticity of scaffolds. We compare three methods for their effects on the biomechanical properties on electrospun scaffolds of poly lactic-co-glycolic acid (PLGA).
Imaging of cells on scaffolds and assessment of production of extracellular matrix (ECM) proteins by cells on scaffolds is described. Culturing cells on scaffolds in vitro can improve scaffold strength and elasticity but the tissue engineering literature shows that cells often fail to produce appropriate ECM when cultured under static conditions. There are few commercial systems available that allow one to culture cells on scaffolds under dynamic conditioning regimes - one example is the Bose Electroforce 3100 which can be used to exert a conditioning programme on cells in scaffolds held using mechanical grips within a media filled chamber.4 An approach to a budget cell culture bioreactor for controlled distortion in 2 dimensions is described. We show that cells can be induced to produce elastin under these conditions. Finally assessment of the biomechanical properties of processed scaffolds cultured with or without cells is described.

Watch this Scientific Journal Video about Postproduction Processing of Electrospun Fibres for Tissue Engineering at JoVE.com

Postproduction Processing of Electrospun Fibres for Tissue Engineering

Electrospun scaffolds के ऊतक इंजीनियरिंग अनुप्रयोगों के लिए पोस्ट उत्पादन संसाधित किया जा सकता है. यहाँ हम जटिल scaffolds के लगातार कताई द्वारा मोटा scaffolds के बहु layering के गर्मी का उपयोग कर या वाष्प annealing के बाँझपन (सड़न रोकनेवाला उत्पादन या नसबंदी के बाद उत्पादन) को प्राप्त करने के लिए और उपयुक्त biomechanical गुणों को प्राप्त करने के लिए, बनाने के लिए, कताई के लिए तरीकों का वर्णन.

Electrospun रेशे के ऊतक इंजीनियरिंग के लिए postproduction प्रसंस्करण

Electrospinning is a commonly used and versatile method to produce  scaffolds (often biodegradable) for 3D tissue engineering.1, 2, 3 Many tissues in vivo ...

Research

JoVE Journal

Bioengineering

Fabrication of Biologically Derived Injectable Materials for Myocardial Tissue Engineering

Myocardial ऊतक इंजीनियरिंग के लिए Biologically व्युत्पन्न इंजेक्शन सामग्री का निर्माण

This protocol provides methods for the preparation of an injectable extracellular matrix (ECM) gel for myocardial tissue engineering applications.  ...

Elastomeric PGS Scaffolds in Arterial Tissue Engineering

Elastomeric PGS धमनी ऊतक इंजीनियरिंग में scaffolds

Cardiovascular disease is one of the leading cause of mortality in the US and especially, coronary artery disease increases with an aging population and ...

Capillary Force Lithography for Cardiac Tissue Engineering

हृदय ऊतक इंजीनियरिंग के लिए केशिका बल लिथोग्राफी

Cardiovascular disease remains the leading cause of death worldwide1. Cardiac tissue engineering holds much promise to deliver groundbreaking medical ...

Design of a Biaxial Mechanical Loading Bioreactor for Tissue Engineering

ऊतक इंजीनियरिंग के लिए एक द्विअक्षीय यांत्रिक लोड हो रहा है बायोरिएक्टर का डिजाइन

We  designed a loading device that is capable of applying uniaxial or biaxial  mechanical strain to a tissue engineered biocomposites fabricated for  ...

Tissue Engineering of a Human 3D in vitro Tumor Test System

Tissue Engineering of a Human 3D in vitro Tumor Test System

एक मानव 3 डी के ऊतक इंजीनियरिंग<em&gt; इन विट्रो</em&gt; ट्यूमर परीक्षा प्रणाली

Cancer is one of the leading causes of death worldwide.  Current therapeutic strategies are predominantly developed in 2D culture  systems, which ...

Electrospun Fibrous Scaffolds of Poly(glycerol-dodecanedioate) for Engineering Neural Tissues From Mouse Embryonic Stem Cells

Electrospun Fibrous Scaffolds of Polyglycerol-dodecanedioate for Engineering Neural Tissues From Mouse Embryonic Stem Cells

माउस भ्रूणीय स्टेम कोशिकाओं से इंजीनियरिंग तंत्रिका ऊतकों के लिए पाली के Electrospun रेशेदार Scaffolds (ग्लिसरॉल dodecanedioate)

For tissue engineering applications, the preparation of biodegradable and biocompatible scaffolds is the most desirable but challenging task. &#160;Among ...

Engineering 3D Cellularized Collagen Gels for Vascular Tissue Regeneration

संवहनी ऊतक उत्थान के लिए इंजीनियरिंग 3 डी Cellularized कोलेजन जैल

Synthetic materials are known to initiate clinical complications such as inflammation, stenosis, and infections when implanted as vascular substitutes. ...

Preparation of Thermoresponsive Nanostructured Surfaces for Tissue Engineering

ऊतक इंजीनियरिंग के लिए Thermoresponsive Nanostructured सतहों की तैयारी

Thermoresponsive poly(N-isopropylacrylamide) (PIPAAm)-immobilized surfaces for controlling cell adhesion and detachment were fabricated by the ...

Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber

Tissue Engineering by Intrinsic Vascularization in an In Vivo Tissue Engineering Chamber

एक में आंतरिक vascularization से ऊतक इंजीनियरिंग<em&gt; Vivo</em&gt; टिशू इंजीनियरिंग चैंबर

In reconstructive surgery, there is a clinical need for an alternative to the current methods of autologous reconstruction which are complex, costly and ...

Isolation of Murine Adipose Tissue-derived Microvascular Fragments as Vascularization Units for Tissue Engineering

ऊतक इंजीनियरिंग के लिए vascularization इकाइयों के रूप में म्यूरीन वसा ऊतक व्युत्पन्न माईक्रवैस्कुलर टुकड़े के अलगाव

A functional microvascular network is of pivotal importance for the survival and integration of engineered tissue constructs. For this purpose, several ...