Name: postproduction processing of electrospun fibres for tissue engineering
Uploaded: 2012-08-09T13:00:00
Description: Watch this Scientific Journal Video about Postproduction Processing of Electrospun Fibres for Tissue Engineering at JoVE.com

Vi takker BBSRC for finansiere en doktorgrad for Mr. Frazer Bye.

1. Electrospinning av tilfeldige og alliansefrie Fibre Electrospinning skaper gode fibrøse nettverk ved hjelp av elektrisk potensial til å tegne en polymer løsning mot jordet oppsamler. Samlere kan være i svært mange former og kan være statisk eller, mer vanlig, roterende. Løsningsmiddelet fordamper før løsningen kommer til solfangeren og jet stivner i en fiber. Hver polymer krever sitt eget sett av betingelser for å produsere en gitt type fiber. Konsentr...

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Electrospinning er en meget populær teknikk for å produsere stillasene for tissue engineering. 14, 15, 16 mens det er relativt enkelt å produsere grunnleggende electrospun stillasene for eksperimentell bruk teknikken er også komplekse og mangefasetterte med mange variabler. 6 Det er mange studier som beskriver hvordan electrospinning parametre bestemme stillaset produsert. I denne studien er fokus på det betydelige utfordringer innlegget produksjonen å gjøre stillasene av hensiktsmessige ark...

<table border="1"><tbody><tr><td> Navn på reagensen </td><td> Firma </td><td> Katalognummer </td><td> Kommentarer </td></tr><tr><td> Poly melkesyre-ko-glykolsyre </td><td> Sigma Aldrich </td><td></td><td></td></tr><tr><td> Poly melkesyre </td><td> Sigma Aldrich </td><td> 81273 </td><td> Iboende viskositet ~ 2.0dl / g </td></tr><tr><td> Poly ε-caprolactone </td><td> Sigma Aldrich </td><td></td><td></td></tr><tr><td> Poly hydroxybutyrate-ko-hydroxyvalerate 12:01 </td><td> Goodfellow </td><td> 578-446-59 </td><td> PHB88/PHV12 </td></tr><tr><td> Dichloromethane </td><td> Sigma Aldrich eller Fisher </td><td> 270 997 eller D/1850/17 </td><td> &gt; 99,8% inneholder 50-150ppm amylene stabilisator </td></tr><tr><td> 50 multi fargede ballonger </td><td> Wilkinsons jernvareforretninger Ltd </td><td> 0105790 </td><td></td></tr><tr><td> Goat anti-kanin IgG (FC): FITC </td><td> AbDserotec </td><td> STAR121F </td><td></td></tr><tr><td> Rabbit anti-human alfa elastin </td><td> AbDserotec </td><td> 4060-1060 </td><td></td></tr><tr><td> Skrukork GL45 PP 2 Port, PK / 2 </td><td> SLS </td><td> 1129750 </td><td></td></tr><tr><td> 4 &#39;,6-Diamidino-2-phenylindole dihydrochloride </td><td> Sigma Aldrich </td><td> 32670 </td><td></td></tr><tr><td> CellTracker grønn CMFDA </td><td> Invitrogen </td><td> C7025 </td><td></td></tr><tr><td> CellTracker rød CMTX </td><td> Invitrogen </td><td> C34552 </td><td></td></tr></tbody></table>

postproduction processing of electrospun fibres for tissue engineering

Electrospinning er et vanlig og allsidig metode for å produsere stillaser (ofte nedbrytbart) for 3D tissue engineering. 1, 2, 3 Mange vev in vivo gjennomgå toaksiale distensjon i varierende grad for eksempel hud, blære, bekkenbunnen og selv den harde gane som barn vokse. I produsere stillasene for disse formålene er det behov for å utvikle stillasene av hensiktsmessige biomekaniske egenskaper (enten oppnås uten eller med celler) og som er sterile for klinisk bruk. Fokuset i denne artikkelen er ikke hvordan å etablere grunnleggende electrospinning parametre (som det er omfattende litteratur om electrospinning), men på hvordan du endrer spunnet stillasene post produksjon slik at de passer for tissue engineering formål - her tykkelse, mekaniske egenskaper og sterilisering (kreves for klinisk bruk) er vurdert og vi også beskrive hvordan celler kan dyrkes på stillasene og utsatt for toaksiale påkjenning å betingelse dem for spesifikke applikasjoner. Electrospinning tendens til å produsere tynne ark, som electrospinning kollektoren blir belagt med isolerende fiber blir det en fattig dirigent slik at fibrene ikke lenger innskudd på den. Derfor vi beskriver tilnærminger til å produsere tykkere konstruksjoner av varme eller damp annealing øke styrken stillasene, men ikke nødvendigvis den elastisitet. Sekvensielle spinning av stillasene av ulike polymerer for å oppnå komplekse Stillasene er også beskrevet. Sterilisering metoder kan påvirke styrke og elastisitet av stillaser. Vi sammenligner tre metoder for deres virkninger på de biomekaniske egenskapene på electrospun stillasene av poly melkesyre-co-glykolsyre (PLGA). Imaging av celler på stillasene og vurdering av produksjon av ekstracellulær matrix (ECM) proteiner i celler på stillasene er beskrevet. Dyrkning celler på stillasene in vitro kan forbedre stillas styrke og elastisitet, men tissue engineering literature viser at cellene ofte ikke klarer å produsere passende ECM når dyrket under statiske betingelser. Det er få kommersielle systemer tilgjengelig tillate at en til kultur celler på stillasene under dynamiske condition regimer -. Ett eksempel er det Bose Electroforce 3100 som kan brukes til å utøve en condition program på celler i stillasene holdt ved hjelp av mekaniske grep i ​​løpet av noen media fylt kammer 4 En tilnærming til et budsjett cellekultur bioreaktor for kontrollert forvrengning i 2 dimensjoner er beskrevet. Vi viser at cellene kan bli overtalt til å produsere elastin under disse forholdene. Endelig vurdering av de biomekaniske egenskapene til bearbeidede stillasene dyrket med eller uten celler er beskrevet.

Watch this Scientific Journal Video about Postproduction Processing of Electrospun Fibres for Tissue Engineering at JoVE.com

Postproduction Processing of Electrospun Fibres for Tissue Engineering

Electrospun stillasene kan behandles etter produksjon for tissue engineering applikasjoner. Her beskriver vi metoder for å spinne komplekse stillasene (med påfølgende sentrifugering), for å lage tykkere stillasene (av multi-lagene ved hjelp av varme eller damp annealing), for å oppnå sterilitet (aseptisk produksjon eller sterilisering post produksjon) og for å oppnå hensiktsmessige biomekaniske egenskaper.

Postproduction Behandling av Electrospun Fibre for Tissue Engineering

Electrospinning is a commonly used and versatile method to produce  scaffolds (often biodegradable) for 3D tissue engineering.1, 2, 3 Many tissues in vivo ...

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Bioengineering

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