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DOI :
10.3791/4182-v
October 2nd, 2012
Chapters
0:05
Title
2:33
BioBrick Assembly
3:37
Alkane Converstion Resting Cell Assay, in vivo
4:46
Alkane Conversion Enzyme Assay, in vitro
6:18
Ethyl Acetate Hydrocarbon Extraction and Concentration Measurements
7:40
Alcohol/Aldehyde Dehydrogenase Activity Assay
8:49
pCaiF Characterization and Tolerance Assay
10:26
Homolog Interaction Mapping
11:11
Representative Hydrocarbon Conversion Results
19:46
Conclusion
一个可持续发展的自动调节修复石油污染的细菌系统的设计采用标准的可互换的DNA部分(BioBricks)。一个工程 E.大肠杆菌菌株用于在有毒的水溶液环境中,通过β-氧化降解烷烃。来自不同物种的酶显示烷烃的降解活性。此外,增加的耐受性 N己烷,通过引入从烷烃耐受菌的基因来实现。
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