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DOI :
10.3791/4216-v
•
13:30 min
November 7th, 2012
Chapters
0:05
Title
1:25
Determining Optimal Heating Conditions
6:15
Generating the Mutant Library
8:44
Soluble Lysate Heat Treatment
10:49
Results: Directed Evolution to Increase the Thermostability of PlyC
13:07
Conclusion
热稳定性工程领域的一种新型的定向进化方法的具体开发,从而验证溶菌酵素。随机诱变只有一轮中,演进式溶菌酶,PlyC 29C3,显示后大于两倍的残留活性相比升高的温度下温育后的野生型蛋白。
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