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DOI :
10.3791/4216-v
•
13:30 min
November 7th, 2012
Chapters
0:05
Title
1:25
Determining Optimal Heating Conditions
6:15
Generating the Mutant Library
8:44
Soluble Lysate Heat Treatment
10:49
Results: Directed Evolution to Increase the Thermostability of PlyC
13:07
Conclusion
耐熱性工学の分野に特有の新奇向進化法は溶菌酵素のために開発され、結果的に検証されました。高温のインキュベーションの後、野生型タンパク質と比較した場合、ランダム突然変異誘発の唯一の1ラウンドの後、進化した溶菌酵素、PlyC 29C3は、二回残存活性よりも大きく表示されます。
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