Name: repair of a critical-sized calvarial defect model using adipose-derived stromal cells harvested from lipoaspirate
Uploaded: 2012-10-31T10:03:00
Description: Watch this Scientific Journal Video about Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate at JoVE.com

Vorremmo ringraziare il Dott. Commons George e il Dr. Dean Vistnes per il loro sostegno e la collaborazione della nostra ricerca. Questo lavoro è supportato dal National Institute of Dental Research e borse di craniofacciale 1 R21 DE019274-01, R01EB009689 e RC2 DE020771-02, la Fondazione di quercia e Hagey Laboratorio di Medicina Rigenerativa di Pediatric MTL Dr. Hyun è supportato dal San Giuseppe Mercy Hospital GME .

1. Cella di isolamento e di espansione <ol><li> Tutti consenso del paziente e protocolli sperimentali sono stati esaminati e approvati dalla Stanford University Institutional Review Board (protocollo # 2188 e # 9999). </li><li> Ottenere umano tessuto adiposo sottocutaneo di procedure elettive di cui lipoaspirazione locale / anestesia generale. </li><li> Ci saranno due strati nella lipoaspirate (Figura 1A). Il surnatante contiene la maggior parte del materiale trattato cellulare. Lo strato inferiore è...

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Poiché l&#39;isolamento di adiposo cellule stromali 2 da lipoaspirate, queste cellule sono state differenziate in una varietà di linee cellulari. Il tessuto adiposo è di origine mesodermica e, quindi, cellule stromali multipotenti derivate da tessuto adiposo sarà probabilmente più efficace con l&#39;applicazione nei confronti di un lignaggio mesodermica. La capacità di generare tessuto scheletrico è particolarmente importante data la carenza di siti donatori per un innesto di osso autologo e le limitaz...

<table border="1"><tbody><tr><td> Nome del reagente: </td><td> Azienda </td><td> Numero di catalogo </td><td> Commenti (opzionale) </td></tr><tr><td></td><td></td><td></td><td> Lipoaspirate Harvest </td></tr><tr><td> PBS </td><td> Gibco </td><td> 10010-023 </td><td></td></tr><tr><td> Sale di Hank soluzione equilibrata </td><td> Cellgro </td><td> 21-023-CV </td><td></td></tr><tr><td> Collagenasi </td><td> Sigma </td><td> C6885-500mg </td><td></td></tr><tr><td> Cella Filtro 100 micron </td><td> BD Falcon </td><td> 352360 </td><td></td></tr><tr><td> Steri-top 500 ml 0,22 micron filtro </td><td> Millipore </td><td> SCGPT05RE </td><td></td></tr><tr><td></td><td></td><td></td><td> Difetto cranica </td></tr><tr><td> Z500 Brushless MicromotorsUM50C </td><td> NSK </td><td> NSKZ500 </td><td></td></tr><tr><td> Circolare Knife 4,0 millimetri </td><td> Xemax chirurgico </td><td> CK40 </td><td></td></tr></tbody></table>

repair of a critical-sized calvarial defect model using adipose-derived stromal cells harvested from lipoaspirate

Craniofacciale scheletrica riparazione e rigenerazione offre la promessa di formazione de novo tessuto attraverso una cella approccio che utilizza cellule staminali. Derivate da tessuto adiposo cellule stromali (CSA) hanno dimostrato di essere una fonte abbondante di cellule staminali multipotenti in grado di subire osteogenico, differenziazione condrogenico, adipogenico e miogenico. Molti studi hanno esplorato il potenziale osteogenico di queste cellule in vivo con l&#39;uso di biomateriali impalcature varie consegna cellulare. È stato dimostrato che utilizzando un osteoconduttiva, rivestimento in idrossiapatite poli (lattico-co-glicolico) (HA-PLGA) scaffold seminati con ASC, un difetto critico dimensioni cranica, un difetto che è definita dalla sua incapacità di subire spontanea guarigione sulla durata di vita dell&#39;animale, può essere efficacemente mostrare robusta rigenerazione ossea. Questo modello in vivo dimostra la base di approcci translazionale volta la rigenerazione del tessuto osseo - il cellularecomponente e matrice biologica. Questo metodo serve da modello per l&#39;applicazione clinico finale di una cellula progenitrice verso la riparazione di un difetto specifico tessuto.

Watch this Scientific Journal Video about Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate at JoVE.com

Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate

Questo protocollo descrive l&#39;isolamento di adiposo cellule stromali di lipoaspirate e la creazione di un millimetro 4 critico imprese difetto cranica per valutare rigenerazione scheletrico.

Riparazione di un critico di dimensioni modello Difetto cranica Usando derivate da tessuto adiposo cellule stromali raccolte da Lipoaspirate

Craniofacial skeletal repair and regeneration offers the promise of de novo tissue formation through a cell-based approach utilizing stem cells. ...

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