Name: repair of a critical-sized calvarial defect model using adipose-derived stromal cells harvested from lipoaspirate
Uploaded: 2012-10-31T10:03:00
Description: Watch this Scientific Journal Video about Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate at JoVE.com

Nos gustaría dar las gracias al Dr. Commons George y el Dr. Dean Vistnes por su apoyo y colaboración de nuestra investigación. Este trabajo es apoyado por el Instituto Nacional de las subvenciones Investigación Dental y Craneofacial 1 DE019274 R21-01, R01EB009689 y DE020771 RC2-02, la Fundación Oak y Laboratorio de Medicina Pediátrica Hagey Regenerativa con MTL Dr. Hyun es apoyado por el Saint Joseph Mercy Hospital GME .

1. Celda de aislamiento y Expansión <ol><li> Todo el consentimiento del paciente y de los protocolos experimentales fueron revisados ​​y aprobados por la Universidad de Stanford Institutional Review Board (Protocolo # 2188 y # 9999). </li><li> Obtención de tejido adiposo subcutáneo humano de los procedimientos electivos bajo lipoaspiración local / anestesia general. </li><li> Habrá dos capas en el lipoaspirado (Figura 1A). El sobrenadante contiene la gran mayoría del material celular procesa...

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Desde el aislamiento de tejido adiposo derivado de células estromales 2 de lipoaspirado, estas células se han diferenciado en una amplia variedad de linajes celulares. El tejido adiposo es de origen mesodérmico y por lo tanto, multipotentes derivadas de tejido adiposo células estromales será probablemente más eficaz con aplicación hacia un linaje mesodérmico. La capacidad de generar tejido óseo es especialmente importante dada la escasez de zonas donantes para un autoinjerto y las limitaciones inhere...

<table border="1"><tbody><tr><td> Nombre del reactivo: </td><td> Empresa </td><td> Número de catálogo </td><td> Comentarios (opcional) </td></tr><tr><td></td><td></td><td></td><td> Lipoaspirado Harvest </td></tr><tr><td> PBS </td><td> Gibco </td><td> 10010-023 </td><td></td></tr><tr><td> Solución salina equilibrada de Hank </td><td> Cellgro </td><td> 21-023 CV- </td><td></td></tr><tr><td> La colagenasa </td><td> Sigma </td><td> C6885-500MG </td><td></td></tr><tr><td> Filtro de células 100 micras </td><td> BD Falcon </td><td> 352360 </td><td></td></tr><tr><td> Steri-top 500 ml 0,22 micras filtro </td><td> Millipore </td><td> SCGPT05RE </td><td></td></tr><tr><td></td><td></td><td></td><td> Defecto del calvario </td></tr><tr><td> Z500 Brushless MicromotorsUM50C </td><td> NSK </td><td> NSKZ500 </td><td></td></tr><tr><td> Circular Knife 4,0 mm </td><td> Xemax quirúrgica </td><td> CK40 </td><td></td></tr></tbody></table>

repair of a critical-sized calvarial defect model using adipose-derived stromal cells harvested from lipoaspirate

Reparación y regeneración del esqueleto craneofacial ofrece la promesa de la formación de tejido de novo a través de un enfoque basado en células utilizando células madre. Derivadas de tejido adiposo células del estroma (ASCs) han demostrado ser una fuente abundante de células madre multipotentes capaces de experimentar diferenciación osteogénica, condrogénicos, adipogénicos, y miogénica. Muchos estudios han explorado el potencial osteogénico de estas células in vivo con el uso de biomateriales andamios diferentes para la entrega celular. Se ha demostrado que mediante la utilización de un osteoconductivos, con recubrimiento de hidroxiapatita poli (láctico-co-glicólico) (PLGA-HA) andamio se sembró con ASC, un defecto de la bóveda craneal de tamaño crítico, un defecto que se define por su incapacidad de someterse espontánea cicatrización durante la vida del animal, se puede mostrar efectivamente la regeneración ósea robusta. Este modelo in vivo demuestra la base de enfoques translacional destinada a regenerar el tejido óseo - el celularcomponente y la matriz biológica. Este método sirve como un modelo para la aplicación clínica final de una célula progenitora hacia la reparación de un defecto específico de tejido.

Watch this Scientific Journal Video about Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate at JoVE.com

Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate

Este protocolo describe el aislamiento de tejido adiposo derivado de células estromales de lipoaspirado y la creación de un 4 mm de tamaño crítico defecto craneal para evaluar la regeneración esquelética.

La reparación de un modelo de defecto crítico del tamaño de calota Uso adiposo derivados de las células del estroma cosechadas desde lipoaspirado

Craniofacial skeletal repair and regeneration offers the promise of de novo tissue formation through a cell-based approach utilizing stem cells. ...

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