Name: repair of a critical-sized calvarial defect model using adipose-derived stromal cells harvested from lipoaspirate
Uploaded: 2012-10-31T10:03:00
Description: Watch this Scientific Journal Video about Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate at JoVE.com

Biz araştırma verdikleri destek ve işbirliği için Dr George Avam ve Dr Dean Vistnes teşekkür etmek istiyorum. Saint Joseph Mercy Hastane GME tarafından desteklenen bu çalışma Diş ve Kraniofasial Araştırma hibeleri 1 R21 DE019274-01, R01EB009689 ve RC2 DE020771-02, MTL Dr Hyun Pediatrik Rejeneratif Tıp Oak Vakfı ve Hagey Laboratuvarı Ulusal Enstitüsü tarafından desteklenmektedir .

1. Hücre İzolasyonu &amp; Genişletme <ol><li> Tüm hastanın rızası ve deneysel protokolleri Stanford Üniversitesi Kurumsal İnceleme Kurulu (Protokol # 2188 ve # 9999) tarafından gözden geçirilmiş ve onaylanmıştır. </li><li> Genel / lokal anestezi altında elektif lipoaspiration prosedürlerden İnsan subkutanöz adipoz dokusu elde edilir. </li><li> Lipoaspirate iki kat (Şekil 1A) olacaktır. Süpernatant işlenmiş hücresel malzemenin büyük bir çoğunluğu içerir. Alt tabaka daha ...

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Ve izolasyonu yağ olarak türetilmiş lipoaspirate dan stromal hücreleri 2, bu hücrelerin hücre soylarının çeşitli ayrışmıştır edilmiştir. Yağ dokusu mezodermal kökenlerden gelen ve bu nedenle, multipotent adipoz-kaynaklı stromal hücrelerin büyük olasılıkla bir mezodermal soy doğru uygulama ile en etkili olacaktır. İskelet doku oluşturmak için yeteneği verici bir otogreft için siteleri ve enfeksiyon, rejeksiyon ve zaman üzerinde 3 arızaları dahil sentetik malzemenin ...

<table border="1"><tbody><tr><td> Reaktif Adı: </td><td> Şirket </td><td> Katalog numarası </td><td> Yorumlar (isteğe bağlı) </td></tr><tr><td></td><td></td><td></td><td> Hasat Lipoaspirate </td></tr><tr><td> PBS </td><td> Gibco </td><td> 10010-023 </td><td></td></tr><tr><td> Hank Dengeli Tuz Çözeltisi </td><td> Cellgro </td><td> 21-023-CV </td><td></td></tr><tr><td> Kollajenaz </td><td> Sigma </td><td> C6885-500MG </td><td></td></tr><tr><td> Hücre Süzgeç 100 mikron </td><td> BD Falcon </td><td> 352360 </td><td></td></tr><tr><td> Steri-500 ml .22 mikron filtre </td><td> Millipore </td><td> SCGPT05RE </td><td></td></tr><tr><td></td><td></td><td></td><td> Kalvaryum Kusur </td></tr><tr><td> Z500 Fırçasız MicromotorsUM50C </td><td> NSK </td><td> NSKZ500 </td><td></td></tr><tr><td> Dairesel Bıçak 4,0 mm </td><td> Xemax Cerrahi </td><td> CK40 </td><td></td></tr></tbody></table>

repair of a critical-sized calvarial defect model using adipose-derived stromal cells harvested from lipoaspirate

Kraniyofasiyal iskelet onarımı ve yenilenmesi kök hücreleri kullanan bir hücre-temelli bir yaklaşım ile de novo doku oluşumu vaadi sunar. Yağ-kaynaklı stromal hücreler (TSK) osteojenik, kondrojenik adipojenik ve miyojenik farklılaşma geçiren yeteneğine multipotent kök hücrelerin bol bir kaynağı olduğu kanıtlanmıştır. Pek çok çalışma hücresel teslim için çeşitli yapı iskelesi biyomalzemelerin kullanımı ile in vivo olarak bu hücrelerin osteojenik potansiyel araştırdılar. Bu gibi olduğu gösterildi bir osteokondüktif, hidroksiapatit-kaplı poli (laktik-ko-glikolik asit) (PLGA-HA) TSK ile iskele tohumlu, bir kritik boyutlu kalvaryal kusur, kendiliğinden geçmesi için kendi yetersizlik ile tanımlanan bir bozukluk kullanılarak , hayvanın ömrü boyunca şifa etkili sağlam kemik rejenerasyon göstermek olabilir. Bu in vivo modelde kemik dokusu yenilenmesine yönelik translasyonel yaklaşım esas göstermektedir - hücreselbileşeni ve biyolojik matrisi. Bu yöntem, bir doku spesifik kusurun tamir yönelik bir projenitör hücre nihai klinik uygulama için bir model olarak hizmet vermektedir.

Watch this Scientific Journal Video about Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate at JoVE.com

Repair of a Critical-sized Calvarial Defect Model Using Adipose-derived Stromal Cells Harvested from Lipoaspirate

Bu protokol izolasyonu açıklar adipoz-türetilmiş lipoaspirate ve iskelet rejenerasyon değerlendirmek için 4 mm kritik ölçüdeki kalvaryal defekt oluşturmadan stromal hücreler.

Lipoaspirate itibaren hasat Yağ kaynaklı stromal hücreler kullanarak Kritik ölçekli Kalvaryum Defekt Model tamiri

Craniofacial skeletal repair and regeneration offers the promise of de novo tissue formation through a cell-based approach utilizing stem cells. ...

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