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DOI :
10.3791/4254-v
October 11th, 2012
Chapters
0:05
Title
1:48
LysoTracker Staining of Mouse Embryos
4:53
LysoTracker Staining of Differentiating ES Cultures
6:51
Mounting Samples for Imaging
7:44
Viewing LysoTracker-stained Samples and Image Analysis
9:21
LysoTracker Staining in Shh Null Embryos and Cultured Cells
12:10
Conclusion
我々は、マウス胚とLysoTrackerと呼ばれる非常に水溶性染料を使用して差別化胚性幹(ES)細胞培養でプログラムされた細胞死(PCD)の領域を可視化するための単純なプロトコルを提示。
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