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DOI :
10.3791/4254-v
October 11th, 2012
Chapters
0:05
Title
1:48
LysoTracker Staining of Mouse Embryos
4:53
LysoTracker Staining of Differentiating ES Cultures
6:51
Mounting Samples for Imaging
7:44
Viewing LysoTracker-stained Samples and Image Analysis
9:21
LysoTracker Staining in Shh Null Embryos and Cultured Cells
12:10
Conclusion
우리는 마우스 배아의 프로그래밍 세포 죽음의 영역 (PCD)와 LysoTracker라는 높은 가용성 염료를 사용하여 배아 줄기 (ES) 세포 문화를 차별화를 시각화하는 간단한 프로토콜을 제시한다.
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