Generazione Substrato per endonucleasi di CRISPR / Cas Sistemi

1:37

CRISPR/Cas System Overview

2:29

Generation of Long Pre-crRNA Substrates via PCR

4:14

Generation of Intermediate Pre-cdRNA Substrates via Annealing of DNA Oligonucleotides

6:10

Generation of Short Cas RNA Substrates via Custom RNA Oligonucleotide Synthesis

6:41

In vitro T7 RNA Polymerase Transcription

8:04

Cas6 Endonuclease Assay

9:08

Results: Representative Cas6 Endonuclease Assay

11:10

Conclusion