Substrate Generation for Endonucleases of CRISPR/Cas SystemsJudith Zoephel 1, Srivatsa Dwarakanath 1, Hagen Richter 1, André Plagens 1, Lennart Randau 1
1Prokaryotic Small RNA Biology, Max-Planck-Institute for Terrestrial Microbiology
CRISPR/Cas systems mediate adaptive immunity in Bacteria and Archaea. Many Cas proteins are proposed to act as endoribonucleases acting on crRNA precursors of varying length. Here we illustrate three different approaches to generate pre-crRNA substrates for the biochemical analysis of Cas endonuclease activity.
