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DOI :
10.3791/4287-v
•
22:49 min
December 13th, 2012
Chapters
0:05
Title
2:19
General Membrane Labeling with PKH26 Cell Tracking Dye
5:43
Flow Cytometer Setup and Staining Verification
7:34
Proliferation Model Selection for Cell Division Monitoring by Dye Dilution
10:16
Results: Optimization of Cell Tracking Dye Staining for Monitoring Cell Division
22:03
Conclusion
免疫細胞機能や増殖を監視するための細胞追跡用染料の使用の成功は、いくつかの重要な手順が含まれます。我々は、するための方法を説明します:1)膜染料と明るく、均一で、再現可能なラベルり得るステップと、2)蛍光色素とデータ収集条件を選択すると、3)色素希釈に基づいて、細胞増殖を定量化するためのモデルを選択する。
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