Name: modeling intracerebral hemorrhage in mice: injection of autologous blood or bacterial collagenase
Uploaded: 2012-09-22T14:00:00
Description: Watch this Scientific Journal Video about Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase at JoVE.com

Denne undersøgelse blev delvist støttet af NIH tilskud RO1NS053407 til JH Zhang. Vi vil gerne takke hr. Damon Klebe for hans værdifulde bidrag.

Alle procedurer blev udført i overensstemmelse med NIH Guide til Pleje og anvendelse af forsøgsdyr og godkendt af Animal Care og brug Udvalg på Loma Linda University. 1. Presurgical Forberedelser Aseptiske teknikker anbefales til alle kirurgiske procedurer. Desinficér stereotaktisk apparat og fremstille sterile kirurgiske redskaber forud for operationen. Bær personlige værnemidler (PPE) under hele håndtering af dyr. Bruge en varmepude under kirurgi ...

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Dyremodeller for intracerebral blødning (ICH) bidrager i høj grad til en avanceret forståelse af sygdommens patofysiologi, og er almindeligt anvendt til at udvikle og evaluere nye terapeutiske strategier i en præklinisk indstilling. Intraparenkymal injektioner af autologt blod eller bakteriel collagenase, er veletablerede metoder til at generere ICH hos gnavere. Begge metoder er oprindelig udviklet i rotter, men på grund af den hurtigt stigende tilgængelighed af transgene og knockout-stammer, mus blev uundværlig ...

<table border="1"><tbody><tr><td> Materiale </td><td> Firma </td><td> Katalognummer </td><td> Kommentar </td></tr><tr><td> Stereotaktisk lampehuset </td><td> Stoelting Co </td><td> 51.600 </td><td></td></tr><tr><td> Nanomite Sprøjtepumpe </td><td> Harvard Apparatus </td><td> PY2 70-2217 </td><td></td></tr><tr><td> Hamilton-sprøjte </td><td> Hamilton Company </td><td> 1725RN (250 ul) 1701 RN (10 pi) </td><td> 26 gauge kanyle til 250 ul og 10 ul sprøjter. </td></tr><tr><td> Microdrill </td><td> Fin Science Tools </td><td> 18.000-17 </td><td></td></tr><tr><td> Microdrill burr </td><td> Fin Science Tools </td><td> 19.007-09 </td><td> 0,9 mm diameter </td></tr><tr><td> Collagenase Type VII-S </td><td> Sigma-Aldrich </td><td> C2399 </td><td></td></tr><tr><td> Microhematocrit kapillarrørene </td><td> Fisher Scientific </td><td> 22-362-574 </td><td> unheparinized </td></tr><tr><td> Knoglevoks </td><td> Ethicon </td><td> W31 </td><td></td></tr><tr><td> Suturere </td><td> Ethicon </td><td> 1676G </td><td></td></tr><tr><td> Ketamin </td><td> JHP Pharmaceuticals </td><td> 42023-115-10 </td><td> Ketalar </td></tr><tr><td> Xylazin </td><td> LLOYD Laboratories </td><td> 139-236 </td><td> AnaSed </td></tr></tbody></table>

modeling intracerebral hemorrhage in mice: injection of autologous blood or bacterial collagenase

Spontan intracerebral blødning (ICH) definerer en potentielt livstruende neurologiske sygdom, der tegner sig for 10-15% af alle slagtilfælde-relaterede indlæggelser og for hvilke der ikke effektive behandlinger er tilgængelige til dato 1,2. På grund af de uensartede ICH hos mennesker er forskellige prækliniske modeller for at grundigt undersøge potentielle terapeutiske strategier 3. Eksperimentel ICH almindeligvis induceres hos gnavere ved intraparenchymal injektion af enten autologt blod eller bakteriel collagenase 4. Den passende model vælges på basis af patofysiologien af ​​blødning induktion og skade progression. Blod injektion model efterligner en hurtigt fremadskridende blødning. Alternativt kan bakteriel collagenase enzymatisk forstyrrer den basale lamina i hjernen kapillærer, hvilket forårsager en aktiv blødning, der almindeligvis udvikler sig over flere timer 5. Resulterende perihematomal ødem og neurofunctional underskud kan kvantificeres from begge modeller. I denne undersøgelse beskrev vi og evalueres en modificeret dobbelt injektion model af autologt blod 6 samt et ICH injektion model af bakteriel collagenase 7, som begge target de basale ganglier (corpus striatum) af mandlige CD-1 mus. Vi vurderede neurofunctional underskud og hjerneødem ved 24 og 72 timer efter ICH induktion. Intrastriatal injektion af autologt blod (30 pl) eller bakteriel collagenase (0.075U) forårsagede reproducerbare neurofunctional underskud hos mus og signifikant forøget hjerne-ødem 24 og 72 timer efter kirurgi (p &lt;0,05). Som konklusion, udbytte begge modeller konsistente hæmorrhagiske infarkter og repræsenterer grundlæggende metoder til præklinisk ICH forskning.

Watch this Scientific Journal Video about Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase at JoVE.com

Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase

Klinisk relevante dyremodeller for intracerebral blødning (ICH) er nødvendige for at udvide kendskabet til hæmoragisk slagtilfælde og til at undersøge nye terapeutiske strategier. I denne undersøgelse beskriver vi og vurdere to ICH-modeller, der implementerer ensidige injektioner af enten autologt blod eller bakteriel collagenase i de basale ganglier (corpus striatum) af mus.

Modellering intracerebral blødning i mus: Injektion af autologt blod eller bakteriel collagenase

Spontaneous intracerebral hemorrhage (ICH) defines a potentially life-threatening neurological malady that accounts for 10-15% of all stroke-related ...

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