Name: modeling intracerebral hemorrhage in mice: injection of autologous blood or bacterial collagenase
Uploaded: 2012-09-22T14:00:00
Description: Watch this Scientific Journal Video about Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase at JoVE.com

Deze studie werd gedeeltelijk ondersteund door NIH subsidie ​​RO1NS053407 aan JH Zhang. Wij willen de heer Damon Klebe bedanken voor zijn waardevolle bijdragen.

Alle procedures werden uitgevoerd in overeenstemming met de NIH Gids voor de zorg en het gebruik van proefdieren en goedgekeurd door de Animal Care en gebruik Comite op Loma Linda University. 1. Pre-operatieve voorbereidingen Aseptische technieken worden aanbevolen voor alle chirurgische ingrepen. Desinfecteer de stereotactische apparaat en alvorens te bereiden steriele chirurgische instrumenten aan de operatie. Draag persoonlijke beschermingsmiddelen (PBM...

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Diermodellen van intracerebrale bloeding (ICH) dragen sterk bij aan een gevorderd begrip van de pathofysiologie van de ziekte, en worden vaak gebruikt voor het ontwikkelen en evalueren van nieuwe therapeutische strategieën in een preklinische setting. Intraparenchymateuze injecties van autoloog bloed of bacteriële collagenase zijn reeds lang gevestigde methoden om ICH bij knaagdieren te genereren. Beide methoden werden oorspronkelijk ontwikkeld in de rat, maar door de snel toenemende beschikbaarheid van transgene en k...

<table border="1"><tbody><tr><td> Materiaal </td><td> Vennootschap </td><td> Catalogus nummer </td><td> Commentaar </td></tr><tr><td> Stereotactische Hoofd Frame </td><td> Stoelting Co </td><td> 51600 </td><td></td></tr><tr><td> Nanomite spuitpomp </td><td> Harvard Apparatus </td><td> PY2 70-2.217 </td><td></td></tr><tr><td> Hamilton spuit </td><td> Hamilton Company </td><td> 1725RN (250 ul) 1701 RN (10 pl) </td><td> 26 Gauge naald voor 250 ul en 10 ul spuiten. </td></tr><tr><td> Microdrill </td><td> Fine Science Tools </td><td> 18.000-17 </td><td></td></tr><tr><td> Microdrill braam </td><td> Fine Science Tools </td><td> 19.007-09 </td><td> 0,9 mm diameter </td></tr><tr><td> Collagenase Type VII-S </td><td> Sigma-Aldrich </td><td> C2399 </td><td></td></tr><tr><td> Microhematocrietmethode Capillaire buizen </td><td> Fisher Scientific </td><td> 22-362-574 </td><td> unheparinized </td></tr><tr><td> Bone Wax </td><td> Ethicon </td><td> W31 </td><td></td></tr><tr><td> Hechten </td><td> Ethicon </td><td> 1676G </td><td></td></tr><tr><td> Ketamine </td><td> JHP Pharmaceuticals </td><td> 42023-115-10 </td><td> Ketalar </td></tr><tr><td> Xylazine </td><td> LLOYD Laboratories </td><td> 139 tot 236 </td><td> AnaSed </td></tr></tbody></table>

modeling intracerebral hemorrhage in mice: injection of autologous blood or bacterial collagenase

Spontane intracerebrale bloeding (ICH) definieert een potentieel levensbedreigende neurologische ziekte die goed is voor 10-15% van alle CVA-gerelateerde ziekenhuisopnamen en waarvoor geen effectieve behandelingen beschikbaar zijn voor 1,2-date. Vanwege de heterogeniteit van ICH bij de mens, zijn verschillende preklinische modellen die nodig zijn om grondig te verkennen potentiële therapeutische strategieën 3. Experimental ICH wordt vaak geïnduceerd in knaagdieren door intraparenchymale injectie van ofwel autoloog bloed of bacteriële collagenase 4. De geschikte model is gekozen gebaseerd op de pathofysiologie van bloeding inductie en progressie schade. Het bloed injectie model bootst een snel voortschrijdende bloeding. U kunt ook bacteriële collagenase enzymatisch verstoort de basale lamina van de hersenen haarvaten, waardoor een actieve bloeding die in het algemeen evolueert gedurende enkele uren 5. Resulterende perihematomal oedeem en neuro-tekorten kunnen weer worden gekwantificeerdm beide modellen. In deze studie beschrijven we een gemodificeerde en geëvalueerd dubbele injectie model van autoloog volbloed 6 als een ICH injectie model van bacteriële collagenase 7, die beide gericht zijn op de basale ganglia (corpus striatum) van mannelijke CD-1 muizen. We neuro-tekorten en hersenoedeem na 24 en 72 uur vastgesteld na ICH inductie. Intrastriatal injectie van autoloog bloed (30 pl) of bacteriële collagenase (0.075U) veroorzaakt reproduceerbaar neuro tekorten in muizen aanzienlijk verhoogd hersenoedeem na 24 en 72 uur na de operatie (p &lt;0,05). Kortom, beide modellen leveren consistente hemorrhagische infarcten en vertegenwoordigen basismethoden voor preklinisch ICH onderzoek.

Watch this Scientific Journal Video about Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase at JoVE.com

Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase

Klinisch relevante diermodellen van intracerebrale bloeding (ICH) zijn nodig om onze kennis van hemorragische beroerte te breiden en nieuwe therapeutische strategieën te onderzoeken. In deze studie beschrijven we twee ICH en beoordelen modellen die eenzijdig injecties van ofwel autoloog volbloed of bacteriële collagenase implementeren in de basale ganglia (corpus striatum) van muizen.

Modeling intracerebrale bloeding in Muizen: Injectie van autoloog bloed of bacterieel collagenase

Spontaneous intracerebral hemorrhage (ICH) defines a potentially life-threatening neurological malady that accounts for 10-15% of all stroke-related ...

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