Name: modeling intracerebral hemorrhage in mice: injection of autologous blood or bacterial collagenase
Uploaded: 2012-09-22T14:00:00
Description: Watch this Scientific Journal Video about Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase at JoVE.com

Questo studio è stato in parte sostenuto da NIH sovvenzione RO1NS053407 a JH Zhang. Vorremmo ringraziare il signor Damon Klebe per i suoi preziosi contributi.

Tutte le procedure sono state effettuate in conformità con la Guida NIH per la cura ed uso di animali da laboratorio e approvato dalla cura degli animali e del Comitato uso della Loma Linda University. 1. Preparazioni prechirurgica Tecniche asettiche sono raccomandati per tutte le procedure chirurgiche. Disinfettare l&#39;apparato stereotassico e preparare sterili strumenti chirurgici prima dell&#39;intervento. Indossare dispositivi di protezione individu...

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Modelli animali di emorragia intracerebrale (ICH) contribuiscono notevolmente a una conoscenza avanzata della fisiopatologia della malattia, e sono comunemente utilizzati per sviluppare e valutare nuove strategie terapeutiche in un ambiente preclinica. Iniezioni intraparenchimali di sangue autologo o collagenasi batterica sono consolidati metodi per generare ICH nei roditori. Entrambi i metodi sono stati sviluppati inizialmente nel ratto, tuttavia, a causa della disponibilità rapida crescita di ceppi transgenici e knoc...

<table border="1"><tbody><tr><td> Materiale </td><td> Azienda </td><td> Numero di catalogo </td><td> Commento </td></tr><tr><td> Testa telaio stereotassica </td><td> Stoelting Co. </td><td> 51600 </td><td></td></tr><tr><td> Nanomite pompa a siringa </td><td> Harvard Apparatus </td><td> PY2 70-2217 </td><td></td></tr><tr><td> Hamilton Siringa </td><td> Hamilton Azienda </td><td> 1725RN (250 microlitri) 1701 RN (10 microlitri) </td><td> 26 gauge per 250 microlitri e 10 siringhe pl. </td></tr><tr><td> Microdrill </td><td> Strumenti Scienza Belle </td><td> 18000-17 </td><td></td></tr><tr><td> Microdrill bava </td><td> Strumenti Scienza Belle </td><td> 19007-09 </td><td> 0,9 millimetri di diametro </td></tr><tr><td> Collagenasi di tipo VII-S </td><td> Sigma-Aldrich </td><td> C2399 </td><td></td></tr><tr><td> Microematocrito tubi capillari </td><td> Fisher Scientific </td><td> 22-362-574 </td><td> unheparinized </td></tr><tr><td> Bone Wax </td><td> Ethicon </td><td> W31 </td><td></td></tr><tr><td> Suturare </td><td> Ethicon </td><td> 1676G </td><td></td></tr><tr><td> Ketamina </td><td> JHP Pharmaceuticals </td><td> 42023-115-10 </td><td> Ketalar </td></tr><tr><td> Xylazina </td><td> LLOYD Laboratories </td><td> 139-236 </td><td> AnaSed </td></tr></tbody></table>

modeling intracerebral hemorrhage in mice: injection of autologous blood or bacterial collagenase

Spontanea emorragia intracerebrale (ICH) definisce una malattia potenzialmente pericolosa per la vita neurologica che rappresenta il 10-15% di tutti i ricoveri legati a ictus e per i quali non sono disponibili trattamenti efficaci ad oggi 1,2. A causa della eterogeneità delle ICH negli esseri umani, diversi modelli preclinici sono necessari per esplorare a fondo i potenziali strategie terapeutiche 3. ICH sperimentale è comunemente indotta in roditori da iniezione intraparenchimale sia di sangue autologo o collagenasi batterica 4. Il modello appropriato viene selezionato in base alla fisiopatologia di induzione emorragie e lesioni progressione. Il modello di iniezione sangue imita una emorragia rapida progressione. In alternativa, collagenasi batterica enzimaticamente sconvolge la lamina basale dei capillari cerebrali, causando un sanguinamento attivo che si evolve in genere per diverse ore 5. Risultante edema perihematomal e deficit neurofunzionali possono essere quantificati from entrambi i modelli. In questo studio abbiamo descritto e valutato un modello modificato doppia iniezione di sangue intero autologo 6 così come un modello ICH iniezione di collagenasi batterica 7, entrambi bersaglio gangli della base (corpo striato) di maschi topi CD-1. Abbiamo valutato deficit neurofunzionali ed edema cerebrale a 24 e 72 ore dopo l&#39;induzione ICH. Intrastriatal iniezione di sangue autologo (30 microlitri) o collagenasi batterica (0.075U) causata riproducibili deficit neurofunzionali in topi e edema cerebrale significativamente aumentata a 24 e 72 ore dopo l&#39;intervento (p &lt;0,05). In conclusione, entrambi i modelli producono consistenti infarti emorragici e rappresentano metodi di base per la ricerca preclinica ICH.

Watch this Scientific Journal Video about Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase at JoVE.com

Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase

Modelli animali clinicamente rilevanti di emorragia intracerebrale (ICH) sono necessari per ampliare la nostra conoscenza di ictus emorragico e di esaminare nuove strategie terapeutiche. In questo studio, abbiamo descrivere e valutare due modelli ICH che implementano iniezioni unilaterali di ciascuna sangue autologo intero o collagenasi batterica nel gangli della base (corpo striato) di topi.

Modellazione emorragia intracerebrale in Mouse: iniezione di sangue autologo o collagenasi batterica

Spontaneous intracerebral hemorrhage (ICH) defines a potentially life-threatening neurological malady that accounts for 10-15% of all stroke-related ...

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Early brain injury and delayed cerebral vasospasm both contribute to unfavorable outcomes after subarachnoid hemorrhage (SAH). Reproducible and ...