Name: modeling intracerebral hemorrhage in mice: injection of autologous blood or bacterial collagenase
Uploaded: 2012-09-22T14:00:00
Description: Watch this Scientific Journal Video about Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase at JoVE.com

Denne studien ble delvis støttet av NIH stipend RO1NS053407 til JH Zhang. Vi vil gjerne takke Mr. Damon Klebe for hans verdifulle bidrag.

Alle prosedyrer ble utført i samsvar med NIH Guide for omsorg og bruk av forsøksdyr og godkjent av Animal Care og bruk komité ved Loma Linda University. 1. Presurgical Forberedelser Aseptiske teknikker anbefales for alle kirurgiske prosedyrer. Desinfisere stereotactic apparater og forberede sterile kirurgiske verktøy før operasjonen. Bruk personlig verneutstyr (PVU) under alle dyr håndtering. Bruk en varmepute under kirurgi for å opprettholde dyrets...

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Dyremodeller av intracerebral blødning (ICH) bidra sterkt til en avansert forståelse av sykdommens patofysiologi, og blir ofte brukt til å utvikle og evaluere nye terapeutiske strategier i en preklinisk innstilling. Intraparenchymal injeksjoner av autologt blod eller bakteriell collagenase er veletablerte metoder for å generere ICH hos gnagere. Begge metoder ble opprinnelig utviklet i rotte, men på grunn av den raskt økende tilgjengelighet av transgene og knockout stammer, mus ble uunnværlig å ytterligere belyse...

<table border="1"><tbody><tr><td> Materiale </td><td> Selskapet </td><td> Katalognummer </td><td> Kommentar </td></tr><tr><td> Stereotactic hodet Frame </td><td> Stoelting Co </td><td> 51600 </td><td></td></tr><tr><td> Nanomite Sprøytepumpe </td><td> Harvard Apparatus </td><td> PY2 70-2217 </td><td></td></tr><tr><td> Hamilton Sprøyte </td><td> Hamilton Selskapet </td><td> 1725RN (250 pl) 1701 RN (10 pl) </td><td> 26 gauge kanyle for 250 pl og 10 pl sprøyter. </td></tr><tr><td> Microdrill </td><td> Fin Science Verktøy </td><td> 18000-17 </td><td></td></tr><tr><td> Microdrill burr </td><td> Fin Science Verktøy </td><td> 19007-09 </td><td> 0,9 mm diameter </td></tr><tr><td> Kollagenase Type VII-S </td><td> Sigma-Aldrich </td><td> C2399 </td><td></td></tr><tr><td> Microhematocrit kapillarrør </td><td> Fisher Scientific </td><td> 22-362-574 </td><td> unheparinized </td></tr><tr><td> Bone Wax </td><td> Ethicon </td><td> W31 </td><td></td></tr><tr><td> Suture </td><td> Ethicon </td><td> 1676G </td><td></td></tr><tr><td> Ketamin </td><td> JHP Pharmaceuticals </td><td> 42023-115-10 </td><td> Ketalar </td></tr><tr><td> Xylazin </td><td> LLOYD Laboratories </td><td> 139-236 </td><td> AnaSed </td></tr></tbody></table>

modeling intracerebral hemorrhage in mice: injection of autologous blood or bacterial collagenase

Spontan intracerebral blødning (ICH) definerer en potensielt livstruende nevrologisk sykdom som står for 10-15% av alle hjerneslag-relaterte sykehusinnleggelser og som ingen effektiv behandling er tilgjengelig for å date 1,2. På grunn av heterogeniteten av ICH hos mennesker, er ulike prekliniske modeller måtte grundig utforske potensielle terapeutiske strategier 3. Eksperimentell ICH er ofte indusert i gnagere ved intraparenchymal injeksjon av enten autologt blod eller bakteriell collagenase 4. Den aktuelle modellen velges basert på patofysiologien for blødning induksjon og skade progresjon. Blodet injeksjon modellen etterligner en raskt framover blødning. Alternativt, bakteriell collagenase enzymatisk forstyrrer basal lamina av hjernen kapillærer, forårsaker en aktiv blødning som vanligvis utvikler seg over flere timer 5. Resulterende perihematomal ødem og neurofunctional underskudd kan kvantifiseres from begge modellene. I denne studien, beskrev vi og evaluert en modifisert dobbelt injeksjon modell av autologt helblod 6 samt en ICH injeksjon modell av bakteriell kollagenase 7, som begge er rettet mot basalgangliene (corpus striatum) av mannlige CD-1 mus. Vi vurderte neurofunctional underskudd og hjerne ødem ved 24 og 72 timer etter ICH induksjon. Intrastriatal injeksjon av autologt blod (30 pl) eller bakteriell collagenase (0.075U) forårsaket reproduserbare neurofunctional underskudd i mus og betydelig økt hjerne ødem på 24 og 72 timer etter operasjonen (p &lt;0,05). I konklusjonen, begge modellene gi konsistente hemoragisk infarkt og representerer grunnleggende metoder for preklinisk ICH forskning.

Watch this Scientific Journal Video about Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase at JoVE.com

Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase

Klinisk relevante dyremodeller av intracerebral blødning (ICH) for å utvide vår kunnskap om hjerneslag og å undersøke nye terapeutiske strategier. I denne studien beskriver vi og vurdere to ICH modeller som implementerer ensidige injeksjoner av enten autologt fullblod eller bakteriell collagenase i basalgangliene (corpus striatum) av mus.

Modellering intracerebral blødning i Mus: Injeksjon av autologt blod eller bakteriell collagenase

Spontaneous intracerebral hemorrhage (ICH) defines a potentially life-threatening neurological malady that accounts for 10-15% of all stroke-related ...

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