Name: modeling intracerebral hemorrhage in mice: injection of autologous blood or bacterial collagenase
Uploaded: 2012-09-22T14:00:00
Description: Watch this Scientific Journal Video about Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase at JoVE.com

Este estudo foi parcialmente financiado pelo NIH concessão RO1NS053407 a JH Zhang. Gostaríamos de agradecer ao Sr. Damon Klebe por suas valiosas contribuições.

Todos os procedimentos foram realizados de acordo com o Guia de NIH para o Cuidado e Uso de Animais de Laboratório e aprovado pelo Comitê Animal Care e Uso da Universidade Loma Linda. 1. Preparação pré-cirúrgica Técnicas assépticas são recomendados para todos os procedimentos cirúrgicos. Desinfectar o aparelho estereotáxico e preparar instrumentos cirúrgicos estéreis antes da cirurgia. Usar equipamento de proteção individual (EPI), ...

<ol>
	<li>Broderick, J. P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Guidelines+for+the+management+of+spontaneous+intracerebral+hemorrhage:+A+statement+for+healthcare+professionals+from+a+special+writing+group+of+the+Stroke+Council,+American+Heart+Association.">Guidelines for the management of spontaneous intracerebral hemorrhage: A statement for healthcare professionals from a special writing group of the Stroke Council, American Heart Association.</a> Stroke. 30, 905-915 (1999).</li><li>Qureshi, A. I., Mendelow, A. D., Hanley, D. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intracerebral+haemorrhage.">Intracerebral haemorrhage.</a> Lancet. 373, 1632-1644 (2009).</li><li>MacLellan, C. L., Silasi, G., Auriat, A. M., Colbourne, F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Rodent+models+of+intracerebral+hemorrhage.">Rodent models of intracerebral hemorrhage.</a> Stroke. 41, 95-98 (2010).</li><li>James, M. L., Warner, D. S., Laskowitz, D. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Preclinical+models+of+intracerebral+hemorrhage:+a+translational+perspective.">Preclinical models of intracerebral hemorrhage: a translational perspective.</a> Neurocrit Care. 9, 139-152 (2008).</li><li>MacLellan, C. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intracerebral+hemorrhage+models+in+rat:+comparing+collagenase+to+blood+infusion.">Intracerebral hemorrhage models in rat: comparing collagenase to blood infusion.</a> J. Cereb. Blood Flow Metab. 28, 516-525 (2008).</li><li>Belayev, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+intracerebral+hemorrhage+in+the+mouse:+histological,+behavioral,+and+hemodynamic+characterization+of+a+double-injection+model.">Experimental intracerebral hemorrhage in the mouse: histological, behavioral, and hemodynamic characterization of a double-injection model.</a> Stroke. 34, 2221-2227 (2003).</li><li>Clark, W., Gunion-Rinker, L., Lessov, N., Hazel, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Citicoline+treatment+for+experimental+intracerebral+hemorrhage+in+mice.">Citicoline treatment for experimental intracerebral hemorrhage in mice.</a> Stroke. 29, 2136-2140 (1998).</li><li>Nakamura, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intracerebral+hemorrhage+in+mice:+model+characterization+and+application+for+genetically+modified+mice.">Intracerebral hemorrhage in mice: model characterization and application for genetically modified mice.</a> J. Cereb. Blood Flow Metab. 24, 487-494 (2004).</li><li>Schallert, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Behavioral+tests+for+preclinical+intervention+assessment.">Behavioral tests for preclinical intervention assessment.</a> NeuroRx. 3, 497-504 (2006).</li><li>Hartman, R., Lekic, T., Rojas, H., Tang, J., Zhang, J. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Assessing+functional+outcomes+following+intracerebral+hemorrhage+in+rats.">Assessing functional outcomes following intracerebral hemorrhage in rats.</a> Brain Res. 1280, 148-157 (2009).</li><li>Hua, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Behavioral+tests+after+intracerebral+hemorrhage+in+the+rat.">Behavioral tests after intracerebral hemorrhage in the rat.</a> Stroke. 33, 2478-2484 (2002).</li><li>Tang, J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mmp-9+deficiency+enhances+collagenase-induced+intracerebral+hemorrhage+and+brain+injury+in+mutant+mice.">Mmp-9 deficiency enhances collagenase-induced intracerebral hemorrhage and brain injury in mutant mice.</a> J. Cereb. Blood Flow Metab. 24, 1133-1145 (2004).</li><li>Ma, Q. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Vascular+adhesion+protein-1+inhibition+provides+antiinflammatory+protection+after+an+intracerebral+hemorrhagic+stroke+in+mice.">Vascular adhesion protein-1 inhibition provides antiinflammatory protection after an intracerebral hemorrhagic stroke in mice.</a> J. Cereb. Blood Flow Metab. , (2010).</li><li>Bullock, R., Mendelow, A. D., Teasdale, G. M., Graham, D. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Intracranial+haemorrhage+induced+at+arterial+pressure+in+the+rat.+Part+1:+Description+of+technique,+ICP+changes+and+neuropathological+findings.">Intracranial haemorrhage induced at arterial pressure in the rat. Part 1: Description of technique, ICP changes and neuropathological findings.</a> Neurol Res. 6, 184-188 (1984).</li><li>Yang, G. Y., Betz, A. L., Chenevert, T. L., Brunberg, J. A., Hoff, J. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Experimental+intracerebral+hemorrhage:+relationship+between+brain+edema,+blood+flow,+and+blood-brain+barrier+permeability+in+rats.">Experimental intracerebral hemorrhage: relationship between brain edema, blood flow, and blood-brain barrier permeability in rats.</a> J. Neurosurg. 81, 93-102 (1994).</li><li>Rosenberg, G. A., Mun-Bryce, S., Wesley, M., Kornfeld, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Collagenase-induced+intracerebral+hemorrhage+in+rats.">Collagenase-induced intracerebral hemorrhage in rats.</a> Stroke. 21, 801-807 (1990).</li><li>Alkayed, N. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gender-linked+brain+injury+in+experimental+stroke.">Gender-linked brain injury in experimental stroke.</a> Stroke. 29, 159-165 (1998).</li><li>Saha, J. K., Xia, J., Grondin, J. M., Engle, S. K., Jakubowski, J. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Acute+hyperglycemia+induced+by+ketamine/xylazine+anesthesia+in+rats:+mechanisms+and+implications+for+preclinical+models.">Acute hyperglycemia induced by ketamine/xylazine anesthesia in rats: mechanisms and implications for preclinical models.</a> Exp Biol Med. (Maywood). 230, 777-784 (2005).</li><li>Fujiwara, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Effect+of+normobaric+oxygen+therapy+in+a+rat+model+of+intracerebral+hemorrhage.">Effect of normobaric oxygen therapy in a rat model of intracerebral hemorrhage.</a> Stroke. 42, 1469-1472 (2011).</li><li>Khatibi, N. H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Isoflurane+posttreatment+reduces+brain+injury+after+an+intracerebral+hemorrhagic+stroke+in+mice.">Isoflurane posttreatment reduces brain injury after an intracerebral hemorrhagic stroke in mice.</a> Anesth Analg. 113, 343-348 (2011).</li></ol>

Modelos animais de hemorragia intracerebral (ICH) contribuem grandemente para um entendimento avançado da fisiopatologia da doença, e são comumente usados ​​para desenvolver e avaliar novas estratégias terapêuticas em um ambiente pré-clínica. Intraparenquimatosos injecções de sangue autólogo ou colagenase bacteriana são bem estabelecidos métodos para a geração de ICH em roedores. Ambos os métodos foram desenvolvidos inicialmente no rato, no entanto, devido à disponibilidade crescente de estirpes tran...

<table border="1"><tbody><tr><td> Material </td><td> Companhia </td><td> Número de Catálogo </td><td> Comentário </td></tr><tr><td> Quadro Chefe estereotáxica </td><td> Stoelting Co. </td><td> 51600 </td><td></td></tr><tr><td> Nanomite bomba de seringa </td><td> Harvard Apparatus </td><td> PY2 70-2217 </td><td></td></tr><tr><td> Hamilton Seringa </td><td> Hamilton Company </td><td> 1725RN (250 ul) 1701 RN (10 ul) </td><td> Agulha de calibre 26 para 250 ul e 10 ul de seringas. </td></tr><tr><td> Microdrill </td><td> Belas Science Tools </td><td> 18000-17 </td><td></td></tr><tr><td> Microdrill rebarba </td><td> Belas Science Tools </td><td> 19007-09 </td><td> 0,9 mm de diâmetro </td></tr><tr><td> Colagenase tipo VII-S </td><td> Sigma-Aldrich </td><td> C2399 </td><td></td></tr><tr><td> Microhematócrito tubos capilares </td><td> Fisher Scientific </td><td> 22-362-574 </td><td> unheparinized </td></tr><tr><td> Cera de osso </td><td> Ethicon </td><td> W31 </td><td></td></tr><tr><td> Suture </td><td> Ethicon </td><td> 1676G </td><td></td></tr><tr><td> Cetamina </td><td> JHP Pharmaceuticals </td><td> 42023-115-10 </td><td> Ketalar </td></tr><tr><td> Xilazina </td><td> LLOYD Laboratories </td><td> 139-236 </td><td> AnaSed </td></tr></tbody></table>

modeling intracerebral hemorrhage in mice: injection of autologous blood or bacterial collagenase

Hemorragia intracerebral espontânea (ICH) define uma doença potencialmente fatal neurológica que responde por 10-15% de todas as internações relacionados ao AVC e para os quais não há tratamentos eficazes estão disponíveis até o momento 1,2. Devido à heterogeneidade do ICH em humanos, vários modelos pré-clínicos são necessários para explorar completamente possíveis estratégias terapêuticas 3. ICH experimental é geralmente induzida em roedores, por injecção ou intraparenquimatosa de sangue autólogo ou colagenase bacteriana 4. O modelo adequado é selecionado com base na fisiopatologia da indução hemorragia e progressão das lesões. O modelo de injeção de sangue imita uma hemorragia evoluindo rapidamente. Alternativamente, a colagenase bacteriana enzimaticamente interrompe a lâmina basal do cérebro capilares, causando uma hemorragia activa, que geralmente se desenvolve ao longo de várias horas 5. Perihematomal edema resultante e déficits neurofuncionais pode ser quantificado from ambos os modelos. Neste estudo, foram descritos e avaliados de um modelo modificado de injecção dupla de sangue total autólogo 6, bem como um modelo de injecção de ICH de colagenase bacteriana 7, ambas as quais alvo os gânglios basais (corpo estriado) do sexo masculino ratinhos CD-1. Foram avaliados os déficits neurofuncionais e edema cerebral em 24 e 72 horas após a indução ICH. Injecção intraestrial de sangue autólogo (30 uL) ou colagenase bacteriana (0.075U) causada reprodutíveis défices neurofuncionais em camundongos e edema cerebral significativamente aumentados em 24 e 72 horas após a cirurgia (p &lt;0,05). Em conclusão, ambos os modelos consistentes produzir infartos hemorrágicos e representam métodos básicos para a pesquisa pré-clínica ICH.

Watch this Scientific Journal Video about Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase at JoVE.com

Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase

Modelos animais clinicamente relevantes de hemorragia intracerebral (ICH) são necessários para ampliar nosso conhecimento sobre AVC hemorrágico e para analisar novas estratégias terapêuticas. Neste estudo, descrever e avaliar dois modelos ICH que implementam injeções unilaterais de tanto sangue autólogo todo ou colagenase bacteriana no gânglio basal (corpo estriado) de camundongos.

Hemorragia modelagem intracerebral em camundongos: Injeção de sangue autólogo ou colagenase bacteriana

Spontaneous intracerebral hemorrhage (ICH) defines a potentially life-threatening neurological malady that accounts for 10-15% of all stroke-related ...

Research

JoVE Journal

Medicine

Modeling Stroke in Mice - Middle Cerebral Artery Occlusion with the Filament Model

Curso de modelagem em Ratos - Oclusão da Artéria Cerebral Média com o modelo de Filamento

Stroke is among the most frequent causes of death and adult disability, especially in highly developed countries. However, treatment options to date are ...

Medicina

Sequencing of Bacterial Microflora in Peripheral Blood: our Experience with HIV-infected Patients

Seqüenciamento da microflora bacteriana no sangue periférico: a nossa experiência com pacientes infectados pelo HIV

The healthy gastrointestinal tract is physiologically colonized by a large variety of commensal microbes that influence the development 
	of the humoral ...

Delivery of Therapeutic Agents Through Intracerebroventricular (ICV) and Intravenous (IV) Injection in Mice

Delivery of Therapeutic Agents Through Intracerebroventricular ICV and Intravenous IV Injection in Mice

Entrega de agentes terapêuticos Através intracerebroventricular (ICV) e injecção intravenosa (IV) em ratos

Despite the protective role that blood brain barrier plays in shielding the brain, it limits the access to the central nervous system (CNS) which most ...

Assessing Changes in Volatile General Anesthetic Sensitivity of Mice after Local or Systemic Pharmacological Intervention

Avaliar as alterações na volátil Geral Anestésica Sensibilidade de Ratos após intervenção farmacológica local ou sistêmica

One desirable endpoint of general anesthesia is  the state of unconsciousness, also known as hypnosis. Defining the hypnotic state in animals is  less ...

Intravital Video Microscopy Measurements of Retinal Blood Flow in Mice

Alterations in retinal blood flow can contribute to, or be a consequence of, ocular disease and visual dysfunction. Therefore, quantitation of altered ...

Intrastriatal Injection of Autologous Blood or Clostridial Collagenase as Murine Models of Intracerebral Hemorrhage

Injection intraestriatal de sangue autólogo ou Clostridial colagenase como modelos murinos de hemorragia intracerebral

Intracerebral hemorrhage (ICH) is a common form of cerebrovascular disease and is associated with significant morbidity and mortality. Lack of effective ...

Modeling Spontaneous Metastatic Renal Cell Carcinoma (mRCC) in Mice Following Nephrectomy

Modeling Spontaneous Metastatic Renal Cell Carcinoma mRCC in Mice Following Nephrectomy

Modelagem espontânea metastático carcinoma de células renais (mRCC) em ratos após nefrectomia

One of the key challenges to improved testing of new experimental therapeutics in renal cell carcinoma (RCC) is the development of models that faithfully ...

Ultrasound-guided Transthoracic Intramyocardial Injection in Mice

Guiada por ultra-som transtorácica intramiocárdico Injection em Ratos

Murine models of cardiovascular disease are important for investigating pathophysiological mechanisms and exploring potential regenerative therapies. ...

Modeling Stroke in Mice: Permanent Coagulation of the Distal Middle Cerebral Artery

Curso de modelagem em Ratos: Coagulação permanente da artéria distal Cerebral Média

Stroke is the third most common cause of death and a main cause of acquired adult disability in developed countries. Only very limited therapeutical ...

The Rabbit Blood-shunt Model for the Study of Acute and Late Sequelae of Subarachnoid Hemorrhage: Technical Aspects

O coelho Modelo Blood-shunt para o Estudo da aguda e tardia Sequelas de hemorragia subaracnóidea: Aspectos Técnicos

Early brain injury and delayed cerebral vasospasm both contribute to unfavorable outcomes after subarachnoid hemorrhage (SAH). Reproducible and ...