Name: modeling intracerebral hemorrhage in mice: injection of autologous blood or bacterial collagenase
Uploaded: 2012-09-22T14:00:00
Description: Watch this Scientific Journal Video about Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase at JoVE.com

Denna studie har delvis stöd av NIH bidrag RO1NS053407 till JH Zhang. Vi vill tacka Mr Damon Klebe för hans värdefulla bidrag.

Alla förfaranden genomfördes i enlighet med NIH Guide för vård och användning av försöksdjur och godkänts av Animal Care och användning kommittén vid Loma Linda University. 1. Presurgical Förberedelser Aseptisk teknik rekommenderas för alla kirurgiska ingrepp. Desinficera stereotaktiska apparater och förbereda sterila kirurgiska instrument före operation. Använd personlig skyddsutrustning (PPE) under hela djurhantering. Använd en värmedyna...

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Djurmodeller av intracerebral blödning (ICH) bidrar starkt till en fördjupad förståelse av sjukdomens patofysiologi, och används ofta för att utveckla och utvärdera nya terapeutiska strategier i en preklinisk miljö. Intraparenkymal injektioner av autologt blod eller bakteriell kollagenas är väletablerade metoder för att generera ICH hos gnagare. Båda metoderna utvecklades ursprungligen på råtta, men på grund av den snabbt ökande tillgången på transgena och knockout stammar, blev mössen nödvändigt at...

<table border="1"><tbody><tr><td> Material </td><td> Företag </td><td> Katalognummer </td><td> Kommentar </td></tr><tr><td> Stereotactic huvudram </td><td> Stoelting Co </td><td> 51.600 </td><td></td></tr><tr><td> Nanomite sprutpump </td><td> Harvard Apparatus </td><td> PY2 70-2217 </td><td></td></tr><tr><td> Hamilton-spruta </td><td> Hamilton Company </td><td> 1725RN (250 pl) 1701 RN (10 pl) </td><td> 26 gauge nål vid 250 ^ och 10 pl sprutor. </td></tr><tr><td> Microdrill </td><td> Fine Science Tools </td><td> 18.000-17 </td><td></td></tr><tr><td> Microdrill burr </td><td> Fine Science Tools </td><td> 19.007-09 </td><td> 0,9 mm i diameter </td></tr><tr><td> Kollagenas typ VII-S </td><td> Sigma-Aldrich </td><td> C2399 </td><td></td></tr><tr><td> Mikrohematokritmetoden kapillärrör </td><td> Fisher Scientific </td><td> 22-362-574 </td><td> unheparinized </td></tr><tr><td> Benvax </td><td> Ethicon </td><td> W31 </td><td></td></tr><tr><td> Sutur </td><td> Ethicon </td><td> 1676G </td><td></td></tr><tr><td> Ketamin </td><td> JHP Pharmaceuticals </td><td> 42023-115-10 </td><td> Ketalar </td></tr><tr><td> Xylazin </td><td> LLOYD Laboratories </td><td> 139-236 </td><td> AnaSed </td></tr></tbody></table>

modeling intracerebral hemorrhage in mice: injection of autologous blood or bacterial collagenase

Spontan intracerebral blödning (ICH) definierar en potentiellt livshotande neurologiska sjukdom som står för 10-15% av alla stroke-relaterade sjukhusinläggningar och för vilka inga effektiva behandlingar finns tillgängliga hittills 1,2. På grund av heterogenitet ICH hos människor, är olika prekliniska modeller som behövs för att noggrant undersöka potentiella terapeutiska strategier 3. Experimentell ICH vanligen framkallas hos gnagare genom intraparenkymal injektion av antingen autologt blod eller bakteriell kollagenas 4. Den lämplig modell väljs utifrån patofysiologi blödning induktion och skada progression. Blodet injektionen Modellen härmar en snabbt progredierande blödning. Alternativt, bakteriell kollagenas stör enzymatiskt basala lamina av hjärnan kapillärerna, vilket en aktiv blödning som i allmänhet utvecklas under flera timmar 5. Resulterande perihematomal ödem och neurofunctional underskott kan kvantifieras tillbakam båda modellerna. I denna studie, som beskrivs vi och utvärderat en modifierad dubbel injektion modell av autologt helblod 6 såväl som en ICH injektion modell av bakteriellt kollagenas 7, vilka båda mål basala ganglierna (corpus striatum) av hankön CD-1-möss. Vi bedömde neurofunctional underskott och hjärnödem vid 24 och 72 timmar efter ICH induktion. Intrastriatal injektion av autologt blod (30 ^ il) eller bakteriellt kollagenas (0.075U) orsakade reproducerbara neurofunctional underskott i möss och ökade signifikant hjärnans ödem vid 24 och 72 timmar efter operationen (p &lt;0,05). Sammanfattningsvis båda modellerna ger konsekventa hemorragiska infarkter och utgör grundläggande metoder för preklinisk ICH forskning.

Watch this Scientific Journal Video about Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase at JoVE.com

Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase

Kliniskt relevanta djurmodeller för intracerebral blödning (ICH) behövs för att utöka vår kunskap om hemorragisk stroke och att undersöka nya terapeutiska strategier. I denna studie beskriver vi och utvärdera två ICH modeller som genomför ensidiga injektioner av antingen autologt helblod eller bakteriell kollagenas i de basala ganglierna (corpus striatum) hos möss.

Modellering intracerebral blödning hos möss: Injektion av autologt blod eller bakteriell Kollagenas

Spontaneous intracerebral hemorrhage (ICH) defines a potentially life-threatening neurological malady that accounts for 10-15% of all stroke-related ...

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