Name: modeling intracerebral hemorrhage in mice: injection of autologous blood or bacterial collagenase
Uploaded: 2012-09-22T14:00:00
Description: Watch this Scientific Journal Video about Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase at JoVE.com

Bu çalışma kısmen JH Zhang NIH hibe RO1NS053407 tarafından desteklenmiştir. Biz onun değerli katkıları için Sayın Damon Klebe teşekkür etmek istiyorum.

Tüm prosedürler Laboratuvar Hayvanlarının Bakımı ve Kullanımı için NIH Kılavuz uygun olarak yürütülmesi ve Loma Linda Üniversitesi Hayvan Bakım ve Kullanım Kurulu tarafından onaylanmıştır. 1. Cerrahi öncesi hazırlıklar Aseptik teknikler tüm cerrahi işlemler için tavsiye edilir. Stereotaktik cihazları dezenfekte ve cerrahi öncesinde steril cerrahi araçları hazırlamak. Tüm hayvan taşıma sırasında kişisel koruyucu ekipman ...

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Intraserebral kanama (İSK) hayvan modelleri hastalığın patofizyolojisinin anlaşılmasına büyük katkı, ve genellikle bir preklinik ortamda yeni tedavi stratejileri geliştirmek ve değerlendirmek için kullanılır. Otolog kan veya bakteriyel kollajenaz intraparankimal enjeksiyonları kemirgenlerde ICH oluşturmak için köklü yöntem vardır. Her iki yöntem başlangıçta sıçan geliştirilmiştir, ancak transgenik ve nakavt suşların hızla artan durumu nedeniyle, fareler daha hemorajik beyin yaralanması ...

<table border="1"><tbody><tr><td> Malzeme </td><td> Şirket </td><td> Katalog Numarası </td><td> Yorumlamak </td></tr><tr><td> Stereotaksik Kafa Çerçeve </td><td> Stoelting Co </td><td> 51600 </td><td></td></tr><tr><td> Nanomit Şırınga Pompası </td><td> Harvard Apparatus </td><td> PY2 70-2217 </td><td></td></tr><tr><td> Hamilton Şırınga </td><td> Hamilton Company </td><td> 1725RN (250 ul) 1701 RN (10 ul) </td><td> 250 ul ve 10 ul şırıngalar için 26 Gauge iğne. </td></tr><tr><td> Mikrotur </td><td> Güzel Bilim Araçları </td><td> 18000-17 </td><td></td></tr><tr><td> Mikrotur çapak </td><td> Güzel Bilim Araçları </td><td> 19007-09 </td><td> 0.9 mm çapında </td></tr><tr><td> Kollajenaz Tip VII-S </td><td> Sigma-Aldrich </td><td> C2399 </td><td></td></tr><tr><td> Kapiler Tüpler Mikrohematokrit </td><td> Fisher Scientific </td><td> 22-362-574 </td><td> heparinize olmayan </td></tr><tr><td> Kemik Wax </td><td> Ethicon </td><td> W31 </td><td></td></tr><tr><td> Dikin </td><td> Ethicon </td><td> 1676G </td><td></td></tr><tr><td> Ketamin </td><td> Jhp İlaç </td><td> 42023-115-10 </td><td> Ketalar </td></tr><tr><td> Ksilazin </td><td> LLOYD Laboratuvarlar </td><td> 139-236 </td><td> AnaSed </td></tr></tbody></table>

modeling intracerebral hemorrhage in mice: injection of autologous blood or bacterial collagenase

Spontan intraserebral kanama (İSK) tüm inme ile ilişkili hastaneye yatışların% 10-15 ve hiçbir etkili tedavi 1,2 bugüne kadar mevcut olduğu için hesapları potansiyel olarak yaşamı tehdit eden nörolojik illet tanımlar. Çünkü insanlarda İSH heterojenliği, çeşitli preklinik modeller iyice 3 prospektif tedavi stratejileri keşfetmek için ihtiyaç vardır. Deneysel ICH yaygın otolog kan ya da bakteriyel ya da kollajenaz 4 arasında intraparenkimal enjeksiyon yoluyla kemirgenlerde meydana gelir. Uygun modeli kanama indüksiyon ve yaralanma ilerleme patofizyolojisi dayanarak seçilir. Kan enjeksiyon modeli bir hızla ilerleyen kanama taklit eder. Alternatif olarak, bakteriyel kollajenaz enzimatik genellikle birkaç saat içindeki 5 geliştikçe aktif kanama neden, beyin kılcal damarların bazal lamina bozar. Sonuç perihematomal ödem ve nöro açıkları fro ölçülebilirm modelleri hem. Bu çalışmada açıklanan ve otolog kan 6 modifiye edilmiş bir çift enjeksiyon modeli hem de bakteri kollajenaz 7&#39;nin bir ICH enjeksiyon modeli, her ikisi de hedef erkek CD-1 mice ve bazal gangliyonlar (corpus striatum). Değerlendirildi Biz ICH indüksiyon sonrası nörofonksiyonel açıkları ve 24. beyin ödemi ve 72 saat değerlendirilmiştir. Otolog kan (30 ul) veya bakteriyel kollajenaz (0.075U) arasında intrastriatal enjeksiyonu farelerde tekrarlanabilir nörofonksiyonel açıkları ve ameliyat sonrası anlamlı artış beynin 24 ödem ve 72 saat (p &lt;0.05) neden oldu. Sonuç olarak, her iki model de tutarlı hemorajik enfarkt verim ve preklinik ICH araştırma için temel yöntemlerini temsil eder.

Watch this Scientific Journal Video about Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase at JoVE.com

Modeling Intracerebral Hemorrhage in Mice: Injection of Autologous Blood or Bacterial Collagenase

Intraserebral kanama (İSK) ve Klinik ilgili hayvan modelleri hemorajik inme bilgimizi genişletmek ve yeni tedavi stratejileri incelemek gereklidir. Bu çalışmada, biz tanımlamak ve farelerin bazal ganglion (korpus striatum) içine otolog tam kan ya da bakteriyel kollajenaz ya tek taraflı enjeksiyon uygulamak iki ICH modelleri değerlendirir.

Farelerde Modelleme İntraserebral Kanama: Otolog Kan veya bakteriyel kollajenaz Enjeksiyon

Spontaneous intracerebral hemorrhage (ICH) defines a potentially life-threatening neurological malady that accounts for 10-15% of all stroke-related ...

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