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DOI :
10.3791/4290-v
•
13:54 min
July 7th, 2012
Chapters
0:05
Title
3:24
Step 1: Preparation of Growth Cones for Seed Germination
5:02
Step 3: Transfer of Seedlings onto the RootChip
3:58
Step 2: Seed Germination and Seedling Growth
6:51
Step 4: Connecting the RootChip to the Carrier
9:18
Step 6: Operating the RootChip using the LabView Interface
8:11
Step 5: Mounting the RootChip at the Microscope
11:09
Results
12:43
Conclusion
この資料では、RootChipのシロイヌナズナ実生の栽培のためのプロトコル、細胞内の代謝産物レベルの微視的なルートのモニタリングおよびFRETベースの測定と成長条件の自動制御を組み合わせたマイクロイメージングプラットフォームを提供します。
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