Name: synthesis of phase-shift nanoemulsions with narrow size distributions for acoustic droplet vaporization and bubble-enhanced ultrasound-mediated ablation
Uploaded: 2012-09-13T12:00:00
Description: Watch this Scientific Journal Video about Synthesis of Phase-shift Nanoemulsions with Narrow Size Distributions for Acoustic Droplet Vaporization and Bubble-enhanced Ultrasound-mediated Ablation at Jo

这项工作是由一个BU / CIMIT应用医疗保健工程博士前奖学金，扩大参与科研启动资助工程（桥接器）美国国家科学基金会，美国国立卫生研究院（R21EB0094930）。

1。制备纳米乳剂相移（PSNE） <ol><li> DPPC将11毫克和1.68毫克DSPE-PEG2000在氯仿</li><li>蒸除有机溶剂中的玻璃圆底烧瓶中，以形成干燥的脂质膜</li><li> Dessicate的脂质膜过夜</li><li>用5.5毫升的磷酸盐缓冲盐水（PBS）再水合的脂质膜</li><li>热解决方案，在45℃水浴中，直到脂膜溶解，涡旋式定期</li><li>转移脂解到7毫升小瓶</li><li>超声脂质溶液，持续2分钟，在20％的振幅</li><li>划分的解决方案分为两小瓶2....

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临床使用高强度聚焦超声（HIFU）热消融肿瘤1为了提高局部加热，并提高热消融在肿瘤中，已经开发了脂质包衣的全氟化碳的液滴可以汽化HIFU。在许多肿瘤的血管异常漏由于其快速增长。2因此，纳米粒子能够穿透窗孔和被动肿瘤内积聚，这个过程被称为增强的渗透性和保持率（EPR）的效果。3它已被证明是在70和200nm之间的纳米粒子最有效地积累在肿瘤。4本报告中?...

<table border="1"><tbody><tr><td> 通用名 </td><td> 生产厂家 </td><td> 猫。数 </td><td> 姓名/说明 </td></tr><tr><td> DPPC </td><td>阿凡提血脂，雪花石膏，AL，USA </td><td> 850355P </td><td> 1,2 - 二棕榈酰-sn-甘油-3 - 磷酸胆碱</td></tr><tr><td> DSPE-PEG2000 </td><td>阿凡提血脂，雪花石膏，AL，USA </td><td> 880120P </td><td> 1,2 - 二硬脂酰-sn-甘油-3 - 磷酸乙醇胺-N-[甲氧基（聚乙二醇）-2000]（铵盐） </td></tr><tr><td> DDFP </td><td> Fluoromed的Round Rock，TX，USA </td><td> CAS号：138495-42-8 </td><td> Dodecafluoropentane（C 5 F 12） </td></tr><tr><td> PBS </td><td> Sigma-Aldrich公司，圣路易斯，MO，USA </td><td> P2194 </td><td>磷酸盐缓冲盐水</td></tr><tr><td>氯仿</td><td> Sigma-Aldrich公司，圣。路易斯，MO，USA </td><td> 372978 </td><td>氯仿</td></tr><tr><td>丙烯酰胺</td><td> Sigma-Aldrich公司，圣路易斯，MO，USA </td><td> A9926 </td><td> 40％，19:1的丙烯酰胺/双丙烯酰胺 - 丙烯酰胺</td></tr><tr><td> Tris缓冲液</td><td> Sigma-Aldrich公司，圣路易斯，MO，USA </td><td> T2694 </td><td> 1M，pH为8，TRIZMA盐酸盐和Trizma碱</td></tr><tr><td> BSA </td><td> Sigma-Aldrich公司，圣路易斯，MO，USA </td><td> A3059 </td><td>牛血清白蛋白</td></tr><tr><td> APS </td><td> Sigma-Aldrich公司，圣路易斯，MO，USA </td><td> A3678 </td><td>过硫酸铵水溶液</td></tr><tr><td> TEMED </td><td> Sigma-Aldrich公司，圣路易斯，MO，USA </td><td> 87689 </td><td> N，N，N&#39;，N&#39;-四甲基乙二胺</td></tr><tr><td></td><td></td><td></td><td> 设备 </td></tr><tr><td>超声发生器（3毫米尖） </TD&gt; <td>超音速材料公司，新城，CT，USA </td><td>振动细胞</td><td></td></tr><tr><td>水浴</td><td>赛默飞世尔科技，美国马萨诸塞州沃尔瑟姆， </td><td> NESLAB EX-7 </td><td></td></tr><tr><td>挤出机</td><td>北血脂，伯纳比，不列颠哥伦比亚省，加拿大</td><td> LIPEX </td><td></td></tr><tr><td>挤出机过滤器</td><td>美国Whatman公司，新泽西州皮斯卡塔韦， </td><td>核孔＃110605和＃110606 </td><td></td></tr><tr><td>挤出机漏光盘</td><td> Sterlitech公司，肯特，WA，USA </td><td> ＃PETEDD25100 </td><td></td></tr><tr><td>塑胶室</td><td>美国塑胶工业股份有限公司，利马，OH，USA </td><td> ＃55288，1 3/16“×1 3/16”×2 7/16“的</td><td></td></tr></tbody></table>

synthesis of phase-shift nanoemulsions with narrow size distributions for acoustic droplet vaporization and bubble-enhanced ultrasound-mediated ablation

高强度聚焦超声（HIFU）是临床上常用的热消融肿瘤。为了提高局部加热和提高热消融在肿瘤中，已经开发了脂质包衣的全氟化碳的液滴可以汽化HIFU。在许多肿瘤的血管由于其快速增长是不正常的漏水，纳米粒子能够渗透开窗和被动积聚在肿瘤。因此，控制液滴的大小，可以导致更好的肿瘤内积累。在这份报告中，稳定的液滴在与窄粒度分布的相移纳米乳（PSNE）的编制说明。 PSNE合成了在全氟化碳液体的存在下，通过超声处理的脂质溶液。一种窄的粒度分布，得到挤出PSNE多次使用与100或200 nm的孔径的过滤器。超过一个7天的期间，使用动态光散射的粒度分布测定。 Polyac在体外实验中，制备含PSNE的​​rylamide水凝胶。 PSNE的水凝胶中的液滴汽化与超声和增强局部加热产生的气泡。汽化PSNE使更多的快速加热，也减少了所需的热消融的超声波强度。因此，PSNE，预计将提高肿瘤热消融治疗中，有可能改善的高强度聚焦超声介导的热消融治疗的治疗效果。

Watch this Scientific Journal Video about Synthesis of Phase-shift Nanoemulsions with Narrow Size Distributions for Acoustic Droplet Vaporization and Bubble-enhanced Ultrasound-mediated Ablation at Jo

Synthesis of Phase-shift Nanoemulsions with Narrow Size Distributions for Acoustic Droplet Vaporization and Bubble-enhanced Ultrasound-mediated Ablation

使用高强度聚焦超声增强局部加热和改善热消融在肿瘤相移纳米乳剂（PSNE）的可以被汽化。在这份报告中，描述的窄粒度分布PSNE稳定的编制。此外，超声介导消融汽化PSNE的影响表现在组织模仿幻影。

相移窄粒度分布的纳米乳液的合成声波液滴蒸发和泡增强的超声介导消融

High-intensity  focused ultrasound (HIFU) is used clinically to thermally ablate tumors. To  enhance localized heating and improve thermal ablation in ...

Research

JoVE Journal

Engineering

Controlling the Size, Shape and Stability of Supramolecular Polymers in Water

控制的大小，形状和稳定性超分子聚合物在水

For aqueous based supramolecular polymers, the simultaneous control over shape, size and stability is very difficult1. At the same time, the ability to do ...

科研

工程学

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

折光传感荧光芯微腔的合成和操作

This paper discusses  fluorescent core microcavity-based sensors that can operate in a microfluidic  analysis setup. These structures are based on the ...

Fabrication, Operation and Flow Visualization in Surface-acoustic-wave-driven Acoustic-counterflow Microfluidics

制造，操作和表面声波驱动声逆流微流体流动可视化

Surface acoustic waves (SAWs) can be used to drive liquids in portable microfluidic chips via the acoustic counterflow phenomenon. In this video we ...

Characterization of Nanocrystal Size Distribution using Raman Spectroscopy with a Multi-particle Phonon Confinement Model

使用拉曼光谱与多颗粒的声子坐月子型号纳米大小分布的特征

Analysis of the size distribution of nanocrystals is a critical requirement for the processing and optimization of their size-dependent properties. The ...

Characterization of Full Set Material Constants and Their Temperature Dependence for Piezoelectric Materials Using Resonant Ultrasound Spectroscopy

全套材料常数表征及其温度依赖性的压电材料使用共振超声波谱

During the operation of high power electromechanical devices, a temperature rise is unavoidable due to mechanical and electrical losses, causing the ...

Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets

基于毛细管的离心微流体装置单分散微滴的大小可控的形成

Here, we demonstrate a simple method for the rapid production of size-controllable, monodisperse, W/O microdroplets using a capillary-based centrifugal ...

Fabrication and Operation of Acoustofluidic Devices Supporting Bulk Acoustic Standing Waves for Sheathless Focusing of Particles

制造和Acoustofluidic设备的运营支撑体声波驻波的聚焦鞘颗粒

Acoustophoresis refers to the displacement of suspended objects in response to directional forces from sound energy. Given that the suspended objects must ...

Measuring Spray Droplet Size from Agricultural Nozzles Using Laser Diffraction

从农业喷嘴采用激光衍射测量喷雾的雾滴尺寸

When making an application of any crop protection material such as an herbicide or pesticide, the applicator uses a variety of skills and information to ...

Preparation of Liquid-exfoliated Transition Metal Dichalcogenide Nanosheets with Controlled Size and Thickness: A State of the Art Protocol

与尺寸可控液体剥离的过渡金属二硫属化物纳米片和厚度的制备：艺术议定书的国家

We summarize recent advances in the production of liquid-exfoliated transition metal dichalcogenide (TMD) nanosheets with controlled size and thickness. ...

A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms

一种基于管尖的液滴微流化平台种子细胞的方法

Amongst various microfluidic platform designs frequently used for cellular analysis, droplet-microfluidics provides a robust tool for isolating and ...