Name: synthesis of phase-shift nanoemulsions with narrow size distributions for acoustic droplet vaporization and bubble-enhanced ultrasound-mediated ablation
Uploaded: 2012-09-13T12:00:00
Description: Watch this Scientific Journal Video about Synthesis of Phase-shift Nanoemulsions with Narrow Size Distributions for Acoustic Droplet Vaporization and Bubble-enhanced Ultrasound-mediated Ablation at Jo

Dette arbejde blev støttet af en BU / CIMIT Applied Healthcare Engineering Predoctoral Fellowship, en National Science Foundation udvide deltagelsen Research Initiation Grant i Engineering (Brige), og National Institutes of Health (R21EB0094930).

1. Fremstilling af Phase-shift nanoemulsion (PSNE) <ol><li> Opløs 11 mg DPPC og 1,68 mg DSPE-PEG2000 i chloroform </li><li> Afdamp det organiske opløsningsmiddel til dannelse af en tør lipidfilm i et glas rundbundet kolbe </li><li> Dessicate lipidfilmen overnight </li><li> Rehydrere lipidfilmen med 5,5 ml phosphatpufret saltvand (PBS) </li><li> Varme opløsning i et 45 ° C vandbad, indtil lipidfilm opløses, vortexbehandling periodisk </li><li> Overfør lipidopløsning i 7 ml hætteglas </li><li> Sonikeres lipidop...

<ol>
	<li>Hynynen, K., Darkazanli, A., Unger, E., Schenck, J. F. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=MRI-guided+noninvasive+ultrasound+surgery.">MRI-guided noninvasive ultrasound surgery.</a> Med. Phys. 20, 107-115 (1993).</li><li>Baban, D. F., Seymour, L. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Control+of+tumour+vascular+permeability.">Control of tumour vascular permeability.</a> Adv. Drug Deliv. Rev. 34, 109-119 (1998).</li><li>Maeda, H., Wu, J., Sawa, T., Matsumura, Y., Hori, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tumor+vascular+permeability+and+the+EPR+effect+in+macromolecular+therapeutics:+a+review.">Tumor vascular permeability and the EPR effect in macromolecular therapeutics: a review.</a> J. Control. Release. 65, 271-284 (2000).</li><li>Schadlich, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Tumor+accumulation+of+NIR+fluorescent+PEG-PLA+nanoparticles:+impact+of+particle+size+and+human+xenograft+tumor+model.">Tumor accumulation of NIR fluorescent PEG-PLA nanoparticles: impact of particle size and human xenograft tumor model.</a> ACS Nano. 5, 8710-8720 (2011).</li><li>Williams, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Convertible+perfluorocarbon+droplets+for+cancer+detection+and+therapy.">Convertible perfluorocarbon droplets for cancer detection and therapy.</a> 2010 IEEE Ultrasonics Symposium. , (2010).</li><li>Martz, T. D., Sheeran, P. S., Bardin, D., Lee, A. P., Dayton, P. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Precision+manufacture+of+phase-change+perfluorocarbon+droplets+using+microfluidics.">Precision manufacture of phase-change perfluorocarbon droplets using microfluidics.</a> Ultrasound Med. Biol. 37, 1952-1957 (2011).</li><li>Giesecke, T., Hynynen, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ultrasound-mediated+cavitation+thresholds+of+liquid+perfluorocarbon+droplets+in+vitro.">Ultrasound-mediated cavitation thresholds of liquid perfluorocarbon droplets in vitro.</a> Ultrasound Med. Biol. 29, 1359-1365 (2003).</li><li>Sheeran, P. S., Luois, S., Dayton, P. A., Matsunaga, T. O. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Formulation+and+Acoustic+Studies+of+a+New+Phase-Shift+Agent+for+Diagnostic+and+Therapeutic+Ultrasound.">Formulation and Acoustic Studies of a New Phase-Shift Agent for Diagnostic and Therapeutic Ultrasound.</a> Langmuir. 27, 10412-10420 (2011).</li><li>Sheeran, P. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Decafluorobutane+as+a+phase-change+contrast+agent+for+low-energy+extravascular+ultrasonic+imaging.">Decafluorobutane as a phase-change contrast agent for low-energy extravascular ultrasonic imaging.</a> Ultrasound Med. Biol. 37, 1518-1530 (2011).</li><li>Zhang, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> The Application of Phase-Shift Nanoemulsion in High Intensity Focused Ultrasound: An In Vitro Study [Doctoral Dissertation]. , (2011).</li><li>Allen, T. M., Hansen, C., Martin, F., Redemann, C., Yau-Young, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Liposomes+containing+synthetic+lipid+derivatives+of+poly(ethylene+glycol)+show+prolonged+circulation+half-lives+in+vivo.">Liposomes containing synthetic lipid derivatives of poly(ethylene glycol) show prolonged circulation half-lives in vivo.</a> Biochim. Biophys. Acta. 1066, 29-36 (1991).</li><li>Klibanov, A. L., Maruyama, K., Beckerleg, A. M., Torchilin, V. P., Huang, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Activity+of+amphipathic+poly(ethylene+glycol)+5000+to+prolong+the+circulation+time+of+liposomes+depends+on+the+liposome+size+and+is+unfavorable+for+immunoliposome+binding+to+target.">Activity of amphipathic poly(ethylene glycol) 5000 to prolong the circulation time of liposomes depends on the liposome size and is unfavorable for immunoliposome binding to target.</a> Biochim. Biophys. Acta. 1062, 142-148 (1991).</li><li>Klibanov, A. L., Maryama, K., Torchilin, V. P., Huang, L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Amphipathic+polyethyleneglycols+effectively+prolong+the+circulation+time+of+liposomes.">Amphipathic polyethyleneglycols effectively prolong the circulation time of liposomes.</a> FEBS Lett. 268, 235-237 (1990).</li><li>Gabizon, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Prolonged+circulation+time+and+enhanced+accumulation+in+malignant+exudates+of+Doxorubicin+encapsulated+in+polyethylene-glycol+coated+liposomes.">Prolonged circulation time and enhanced accumulation in malignant exudates of Doxorubicin encapsulated in polyethylene-glycol coated liposomes.</a> Cancer Res. 54, 987-992 (1994).</li><li>Awasthi, V. D., Garcia, D., Goins, B. A., Philips, W. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Circulation+and+biodistribution+profiles+of+long-circulating+PEG-liposomes+of+various+sizes+in+rabbits.">Circulation and biodistribution profiles of long-circulating PEG-liposomes of various sizes in rabbits.</a> Int. J. Pharm. 253, 121-132 (2003).</li><li>Zhang, P., Porter, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=An+in+vitro+study+of+a+phase-shift+nanoemulsion:+a+potential+nucleation+agent+for+bubble-enhanced+HIFU+tumor+ablation.">An in vitro study of a phase-shift nanoemulsion: a potential nucleation agent for bubble-enhanced HIFU tumor ablation.</a> Ultrasound Med. Biol. 36, 1856-1866 (2010).</li><li>Lafon, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gel+phantom+for+use+in+high-intensity+focused+ultrasound+dosimetry.">Gel phantom for use in high-intensity focused ultrasound dosimetry.</a> Ultrasound Med. Biol. 31, 1383-1389 (2005).</li></ol>

Høj intensitet fokuseret ultralyd (HIFU) anvendes klinisk til termisk ablatere tumorer. 1 For at forbedre lokal opvarmning og forbedre termisk ablation i tumorer, har lipid-belagt perfluorcarbongasser dråber blevet udviklet, som kan fordampes ved HIFU. Vaskulaturen i mange tumorer er unormalt utæt på grund af deres hurtige vækst. 2 således nanopartikler er i stand til at trænge ind i fenestrationer og passivt akkumuleres i tumorer, en proces kendt som den forøgede permeabilitet og retention...

<table border="1"><tbody><tr><td> Almindeligt navn </td><td> Producent </td><td> Kat. Nummer </td><td> Fulde navn / beskrivelse </td></tr><tr><td> DPPC </td><td> Avanti Lipids, Alabaster, AL, USA </td><td> 850355P </td><td> 1,2-dipalmitoyl-sn-glycero-3-phosphocholin </td></tr><tr><td> DSPE-PEG2000 </td><td> Avanti Lipids, Alabaster, AL, USA </td><td> 880120P </td><td> 1,2-distearoyl-sn-glycero-3-phosphoethanolamin-N-[methoxy (polyethylenglycol) -2000] (ammoniumsalt) </td></tr><tr><td> DDFP </td><td> Fluoromed, Round Rock, TX, USA </td><td> CAS: 138495-42-8 </td><td> Dodecafluorpentan (C 5 F 12) </td></tr><tr><td> PBS </td><td> Sigma-Aldrich, St. Louis, MO, USA </td><td> P2194 </td><td> Phosphatpufret saltvand </td></tr><tr><td> Chloroform </td><td> Sigma-Aldrich, St. Louis, MO, USA </td><td> 372.978 </td><td> Chloroform </td></tr><tr><td> Akrylamid </td><td> Sigma-Aldrich, St. Louis, MO, USA </td><td> A9926 </td><td> 40% 19:01 acrylamid / bis-acrylamid </td></tr><tr><td> Tris-puffer </td><td> Sigma-Aldrich, St. Louis, MO, USA </td><td> T2694 </td><td> 1M, pH 8, Trizma-hydrochlorid og Trizma-base </td></tr><tr><td> BSA </td><td> Sigma-Aldrich, St. Louis, MO, USA </td><td> A3059 </td><td> Bovint serumalbumin </td></tr><tr><td> APS </td><td> Sigma-Aldrich, St. Louis, MO, USA </td><td> A3678 </td><td> Ammoniumpersulfatopløsning </td></tr><tr><td> TEMED </td><td> Sigma-Aldrich, St. Louis, MO, USA </td><td> 87.689 </td><td> N, N, N &#39;, N&#39;-tetramethylethylendiamin </td></tr><tr><td></td><td></td><td></td><td> Udstyr </td></tr><tr><td> Sonikator (3 mm spids) </td&gt; <td> Sonics &amp; Materials, Inc., Newtown, CT, USA </td><td> Vibra-Cell </td><td></td></tr><tr><td> Vandbad </td><td> Thermo Fisher Scientific, Waltham, MA, USA </td><td> Neslab EX-7 </td><td></td></tr><tr><td> Ekstruder </td><td> Northern Lipids, Burnaby, BC, Canada </td><td> Lipex </td><td></td></tr><tr><td> Ekstruder Filtre </td><td> Whatman, Piscataway, NJ, USA </td><td> Nuclepore # 110.605 og # 110.606 </td><td></td></tr><tr><td> Extruder Drain Disc </td><td> Sterlitech Corporation, Kent, WA, USA </td><td> # PETEDD25100 </td><td></td></tr><tr><td> Plastic kammer </td><td> U.S. Plastic Corporation, Lima, OH, USA </td><td> # 55.288, 1 3/16 &quot;x1 3/16&quot; x2 7/16 &quot; </td><td></td></tr></tbody></table>

synthesis of phase-shift nanoemulsions with narrow size distributions for acoustic droplet vaporization and bubble-enhanced ultrasound-mediated ablation

Høj intensitet fokuseret ultralyd (HIFU) anvendes klinisk til termisk ablatere tumorer. At øge lokal opvarmning og forbedre termisk ablation i tumorer, er lipid-overtrukne perfluorcarbongasser smådråber blevet udviklet, som kan fordampes ved HIFU. Vaskulaturen i mange tumorer er unormalt utæt på grund af deres hurtige vækst og nanopartikler kan trænge ind i fenestrationer og passivt akkumuleres i tumorer. Således kan styring af størrelsen af ​​smådråberne resultere i bedre akkumulering i tumorer. I denne rapport er fremstillingen af ​​stabile dråber i en faseforskydning nanoemulsion (PSNE) med en snæver størrelsesfordeling beskrevet. PSNE blev syntetiseret ved sonikering af en lipidopløsning i nærværelse af flydende carbonperfluorid. En snæver størrelsesfordeling blev fremstillet ved at ekstrudere de PSNE flere gange under anvendelse af filtre med porestørrelser på 100 eller 200 nm. Størrelsesfordelingen blev målt over en periode på 7 dage ved hjælp af dynamisk lysspredning. Polyacrylamide hydrogeler indeholdende PSNE blev fremstillet til in vitro forsøg. PSNE smådråber i hydrogelerne blev fordampet med ultralyd og de resulterende bobler forbedret lokal opvarmning. Fordampet PSNE muliggør hurtigere opvarmning og reducerer også ultralyd intensitet nødvendig for termisk ablation. Således er PSNE forventes at forbedre termisk ablation i tumorer, potentielt forbedre terapeutiske resultater af HIFU-medierede termisk ablation behandlinger.

Watch this Scientific Journal Video about Synthesis of Phase-shift Nanoemulsions with Narrow Size Distributions for Acoustic Droplet Vaporization and Bubble-enhanced Ultrasound-mediated Ablation at Jo

Synthesis of Phase-shift Nanoemulsions with Narrow Size Distributions for Acoustic Droplet Vaporization and Bubble-enhanced Ultrasound-mediated Ablation

Phase-shift nanoemulsions (PSNE) kan fordampes ved hjælp af høj intensitet fokuseret ultralyd til at forbedre lokal opvarmning og forbedre termisk ablation i tumorer. I denne rapport, er fremstillingen af ​​stabile PSNE med en snæver størrelsesfordeling beskrevet. Endvidere er effekten af ​​fordampet PSNE på ultralyd-medieret ablation påvist i væv der efterligner fantomer.

Syntese af Phase-shift nanoemulsions med smalle størrelse distributioner til Akustisk Droplet fordampning og Bubble-forstærket Ultralyd-medieret Ablation

High-intensity  focused ultrasound (HIFU) is used clinically to thermally ablate tumors. To  enhance localized heating and improve thermal ablation in ...

Research

JoVE Journal

Engineering

Controlling the Size, Shape and Stability of Supramolecular Polymers in Water

Styring af størrelse, form og stabilitet af supramolekylære polymerer i vand

For aqueous based supramolecular polymers, the simultaneous control over shape, size and stability is very difficult1. At the same time, the ability to do ...

Synthesis and Operation of Fluorescent-core Microcavities for Refractometric Sensing

Syntese og drift af fluorescerende-core mikrokaviteter for refraktometriske Sensing

This paper discusses  fluorescent core microcavity-based sensors that can operate in a microfluidic  analysis setup. These structures are based on the ...

Fabrication, Operation and Flow Visualization in Surface-acoustic-wave-driven Acoustic-counterflow Microfluidics

Fabrikation, Drift og Flow Visualisering i Overflade-akustisk-bølgedrevne Acoustic-modstrøms Microfluidics

Surface acoustic waves (SAWs) can be used to drive liquids in portable microfluidic chips via the acoustic counterflow phenomenon. In this video we ...

Characterization of Nanocrystal Size Distribution using Raman Spectroscopy with a Multi-particle Phonon Confinement Model

Karakterisering af Nanocrystal størrelsesfordeling hjælp Raman spektroskopi med en Multi-partikel Phonon Indespærring Model

Analysis of the size distribution of nanocrystals is a critical requirement for the processing and optimization of their size-dependent properties. The ...

Characterization of Full Set Material Constants and Their Temperature Dependence for Piezoelectric Materials Using Resonant Ultrasound Spectroscopy

Karakterisering af komplet sæt Materiale konstanter og deres temperatur Afhængighed for piezoelektriske materialer Brug Resonant Ultralyd spektroskopi

During the operation of high power electromechanical devices, a temperature rise is unavoidable due to mechanical and electrical losses, causing the ...

Capillary-based Centrifugal Microfluidic Device for Size-controllable Formation of Monodisperse Microdroplets

Kapillær-baserede Centrifugal mikrofluidanordning for Size-styrbar Dannelse monodisperse Mikrodråber

Here, we demonstrate a simple method for the rapid production of size-controllable, monodisperse, W/O microdroplets using a capillary-based centrifugal ...

Fabrication and Operation of Acoustofluidic Devices Supporting Bulk Acoustic Standing Waves for Sheathless Focusing of Particles

Fabrikation og drift af Acoustofluidic Devices Støtte Bulk Acoustic stående bølger for Sheathless Fokusering af partikler

Acoustophoresis refers to the displacement of suspended objects in response to directional forces from sound energy. Given that the suspended objects must ...

Measuring Spray Droplet Size from Agricultural Nozzles Using Laser Diffraction

Måling Spray Droplet Størrelse fra Jordbrugsvidenskabelige Dyser Brug Laser Diffraktion

When making an application of any crop protection material such as an herbicide or pesticide, the applicator uses a variety of skills and information to ...

Preparation of Liquid-exfoliated Transition Metal Dichalcogenide Nanosheets with Controlled Size and Thickness: A State of the Art Protocol

Udarbejdelse af Liquid-afstødes Transition Metal Dichalcogenide Nanosheets med kontrolleret størrelse og tykkelse: En State of the Art protokollen

We summarize recent advances in the production of liquid-exfoliated transition metal dichalcogenide (TMD) nanosheets with controlled size and thickness. ...

A Pipette-Tip Based Method for Seeding Cells to Droplet Microfluidic Platforms

En Pipette-Tip baseret metode for såning celler til dråbe mikrofluid platforme

Amongst various microfluidic platform designs frequently used for cellular analysis, droplet-microfluidics provides a robust tool for isolating and ...