Name: synthesis of phase-shift nanoemulsions with narrow size distributions for acoustic droplet vaporization and bubble-enhanced ultrasound-mediated ablation
Uploaded: 2012-09-13T12:00:00
Description: Watch this Scientific Journal Video about Synthesis of Phase-shift Nanoemulsions with Narrow Size Distributions for Acoustic Droplet Vaporization and Bubble-enhanced Ultrasound-mediated Ablation at Jo

Dit werk werd ondersteund door een BU / CIMIT Toegepaste Gezondheidszorg Techniek Predoctoraal Fellowship, een National Science Foundation Verbreding van de deelname Onderzoek Initiatie Grant in de ingenieurswetenschappen (BRIGE) en de National Institutes of Health (R21EB0094930).

1. Voorbereiding van Phase-shift Nanoemulsion (PSNE) <ol><li> Los 11 mg DPPC en 1,68 mg DSPE-PEG2000 in chloroform </li><li> Damp het organisch oplosmiddel om een ​​droge lipide film vormen in een glazen rondbodemkolf </li><li> &#39;S nachts Dessicate de lipide film </li><li> Hydrateren de lipidefilm met 5,5 ml fosfaat-gebufferde zoutoplossing (PBS) </li><li> Warmte oplossing in een 45 ° C waterbad lipidefilm oplost, vortexen periodiek </li><li> Breng lipideoplossing in 7 ml flacon </li><li> Sonificeer lipideoplo...

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High-intensity focused ultrasound (HIFU) wordt klinisch gebruikt om thermisch ablatie tumoren. 1 Om plaatselijke verwarming te versterken en te verbeteren thermische ablatie in tumoren, hebben lipiden gecoate perfluorkoolstof druppels ontwikkeld die kunnen worden verdampt door HIFU. De bloedvaten in veel tumoren abnormaal lek vanwege hun snelle groei. &#39;2, Nanodeeltjes kunnen de fenestraties dringen en passief verzamelen in tumoren, een proces dat de verhoogde permeabiliteit en retentie (EPR) ef...

<table border="1"><tbody><tr><td> Common Name </td><td> Fabrikant </td><td> Cat. Aantal </td><td> Volledige Naam / Beschrijving </td></tr><tr><td> DPPC </td><td> Avanti Lipiden, Alabaster, AL, Verenigde Staten </td><td> 850355P </td><td> 1,2-dipalmitoyl-sn-glycero-3-fosfocholine </td></tr><tr><td> DSPE-PEG2000 </td><td> Avanti Lipiden, Alabaster, AL, Verenigde Staten </td><td> 880120P </td><td> 1,2-distearoyl-sn-glycero-3-fosfoethanolamine-N-[methoxy (polyethyleenglycol) -2,000] (ammoniumzout) </td></tr><tr><td> DDFP </td><td> Fluoromed, Round Rock, TX, Verenigde Staten </td><td> CAS: 138495-42-8 </td><td> Dodecafluorpentaan (C 5 F 12) </td></tr><tr><td> PBS </td><td> Sigma-Aldrich, St. Louis, MO, USA </td><td> P2194 </td><td> Fosfaat gebufferde zoutoplossing </td></tr><tr><td> Chloroform </td><td> Sigma-Aldrich, St. Louis, MO, USA </td><td> 372978 </td><td> Chloroform </td></tr><tr><td> Acrylamide </td><td> Sigma-Aldrich, St. Louis, MO, USA </td><td> A9926 </td><td> 40% 19:1 acrylamide / bis-acrylamide </td></tr><tr><td> Tris buffer </td><td> Sigma-Aldrich, St. Louis, MO, USA </td><td> T2694 </td><td> 1M, pH 8, Trizma hydrochloride en Trizma base </td></tr><tr><td> BSA </td><td> Sigma-Aldrich, St. Louis, MO, USA </td><td> A3059 </td><td> Runderserumalbumine </td></tr><tr><td> APS </td><td> Sigma-Aldrich, St. Louis, MO, USA </td><td> A3678 </td><td> Ammoniumpersulfaat oplossing </td></tr><tr><td> TEMED </td><td> Sigma-Aldrich, St. Louis, MO, USA </td><td> 87689 </td><td> N, N, N &#39;, N&#39;-tetramethylethyleendiamine </td></tr><tr><td></td><td></td><td></td><td> Uitrusting </td></tr><tr><td> Sonicator (3 mm tip) </td&gt; <td> Sonics &amp; Materials, Inc, Newtown, CT, USA </td><td> Vibra-Cell </td><td></td></tr><tr><td> Waterbad </td><td> Thermo Fisher Scientific, Waltham, MA, Verenigde Staten </td><td> Neslab EX-7 </td><td></td></tr><tr><td> Extruder </td><td> Noord-Lipiden, Burnaby, BC, Canada </td><td> Lipex </td><td></td></tr><tr><td> Extruder Filters </td><td> Whatman, Piscataway, NJ, USA </td><td> Nuclepore # 110605 en # 110606 </td><td></td></tr><tr><td> Extruder Drain Disc </td><td> Sterlitech Corporation, Kent, WA, Verenigde Staten </td><td> # PETEDD25100 </td><td></td></tr><tr><td> Plastic kamer </td><td> VS Plastic Corporation, Lima, OH, Verenigde Staten </td><td> # 55288, 1 3/16 &quot;x1 3/16&quot; x2 7/16 &quot; </td><td></td></tr></tbody></table>

synthesis of phase-shift nanoemulsions with narrow size distributions for acoustic droplet vaporization and bubble-enhanced ultrasound-mediated ablation

High-intensity focused ultrasound (HIFU) wordt klinisch gebruikt om thermisch ablatie tumoren. Om plaatselijke verwarming verhogen en thermische ablatie in tumoren, zijn lipiden gecoate perfluorkoolstof druppeltjes ontwikkeld die worden verdampt door HIFU. De bloedvaten in veel tumoren abnormaal lek vanwege hun snelle groei en nanodeeltjes kunnen de fenestraties dringen en passief accumuleren in tumoren. Zo kan het regelen van de grootte van de druppeltjes tot een betere accumulatie in tumoren. In dit rapport wordt de bereiding van stabiele druppeltjes in een faseverschuiving nanoemulsion (PSNE) met een smalle grootteverdeling beschreven. PSNE werden gesynthetiseerd door sonicatie een lipideoplossing in aanwezigheid van vloeistof perfluorkoolstof. Een smalle grootteverdeling werd verkregen door het extruderen van de PSNE meerdere keren met filters met poriegrootten van 100 of 200 nm. De grootteverdeling werd gemeten over een periode van 7 dagen met behulp van dynamische lichtverstrooiing. Polyacrylamide hydrogelen met PSNE werden voorbereid voor in vitro experimenten. PSNE druppeltjes in de hydrogelen werden verdampt met ultrageluid en het resulterende bellen versterkt plaatselijke verhitting. Verdampte PSNE laat sneller verwarmen en vermindert ook de ultrasone intensiteit nodig voor thermische ablatie. Aldus wordt verwacht PSNE thermische ablatie in tumoren bevorderen, mogelijk verbeteren van therapeutische resultaten van HIFU gemedieerde thermische ablatie behandelingen.

Watch this Scientific Journal Video about Synthesis of Phase-shift Nanoemulsions with Narrow Size Distributions for Acoustic Droplet Vaporization and Bubble-enhanced Ultrasound-mediated Ablation at Jo

Synthesis of Phase-shift Nanoemulsions with Narrow Size Distributions for Acoustic Droplet Vaporization and Bubble-enhanced Ultrasound-mediated Ablation

Faseverschuiving nanoemulsions (PSNE) kan worden verdampt met behulp van High Intensity Focused Ultrasound bij plaatselijke verwarming te verbeteren en thermische ablatie in tumoren te verbeteren. In dit rapport wordt de bereiding van stabiele PSNE met een smalle grootteverdeling beschreven. Bovendien is de impact van de verdampte PSNE op echografie-gemedieerde ablatie aangetoond in weefsel-nabootsende fantomen.

Synthese van Phase-shift Nanoemulsions met smalle Grootte Uitkeringen voor akoestische Droplet-verdamping en Bubble-enhanced Ultrasound-gemedieerde Ablation

High-intensity  focused ultrasound (HIFU) is used clinically to thermally ablate tumors. To  enhance localized heating and improve thermal ablation in ...

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