Name: synthesis of phase-shift nanoemulsions with narrow size distributions for acoustic droplet vaporization and bubble-enhanced ultrasound-mediated ablation
Uploaded: 2012-09-13T12:00:00
Description: Watch this Scientific Journal Video about Synthesis of Phase-shift Nanoemulsions with Narrow Size Distributions for Acoustic Droplet Vaporization and Bubble-enhanced Ultrasound-mediated Ablation at Jo

Dette arbeidet ble støttet av en BU / CIMIT Applied Healthcare Engineering Predoctoral Fellowship, en National Science Foundation Utvide Deltakelse Forskning Initiation Grant i Engineering (Brige), og National Institutes of Health (R21EB0094930).

1. Utarbeidelse av fase-shift nanoemulsion (PSNE) <ol><li> Oppløs 11 mg DPPC og 1,68 mg DSPE-PEG2000 i kloroform </li><li> Fordamp det organiske oppløsningsmiddel for å danne en tørr lipidfilm i et glass rundkolbe </li><li> Dessicate lipid film over natten </li><li> Rehydrere lipidfilmen med 5,5 ml fosfat-bufret saltvann (PBS) </li><li> Heat oppløsning i et 45 ° C vannbad inntil lipidfilm oppløses, vortexblanding periodisk </li><li> Overfør lipidoppløsningen inn 7 ml hetteglass </li><li> Sonicate lipidoppløs...

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Høyintensitets fokusert ultralyd (HIFU) brukes klinisk til termisk ablate svulster. 1 For å forbedre lokal oppvarmning og forbedre termisk ablasjon i tumorer, har lipid-belagt perfluorocarbon dråper blitt utviklet som kan fordampes ved HIFU. Blodkar i mange tumorer er unormalt utett på grunn av deres raske vekst. 2 Dermed nanopartikler er i stand til å trenge gjennom fenestrations og passivt akkumuleres i tumorer, en prosess kjent som forbedret permeabilitet og retensjon (EPR) effekt. 3</...

<table border="1"><tbody><tr><td> Vanlig navn </td><td> Produsent </td><td> Kat. Nummer </td><td> Fullt navn / beskrivelse </td></tr><tr><td> DPPC </td><td> Avanti Lipider, Alabaster, AL, USA </td><td> 850355P </td><td> 1,2-dipalmitoyl-sn-glycero-3-phosphocholine </td></tr><tr><td> DSPE-PEG2000 </td><td> Avanti Lipider, Alabaster, AL, USA </td><td> 880120P </td><td> 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[metoksy (polyetylenglykol) -2000] (ammoniumsalt) </td></tr><tr><td> DDFP </td><td> Fluoromed, Round Rock, TX, USA </td><td> CAS: 138495-42-8 </td><td> Dodecafluoropentane (C 5 F 12) </td></tr><tr><td> PBS </td><td> Sigma-Aldrich, St. Louis, MO, USA </td><td> P2194 </td><td> Fosfat-bufret saltvann </td></tr><tr><td> Kloroform </td><td> Sigma-Aldrich, St. Louis, MO, USA </td><td> 372978 </td><td> Kloroform </td></tr><tr><td> Akrylamid </td><td> Sigma-Aldrich, St. Louis, MO, USA </td><td> A9926 </td><td> 40% 19:01 akrylamid / bis-akrylamid </td></tr><tr><td> Tris-buffer </td><td> Sigma-Aldrich, St. Louis, MO, USA </td><td> T2694 </td><td> 1M, pH 8, Trizma hydroklorid og Trizma base </td></tr><tr><td> BSA </td><td> Sigma-Aldrich, St. Louis, MO, USA </td><td> A3059 </td><td> Bovint serumalbumin </td></tr><tr><td> APS </td><td> Sigma-Aldrich, St. Louis, MO, USA </td><td> A3678 </td><td> Ammoniumpersulfat løsning </td></tr><tr><td> TEMED </td><td> Sigma-Aldrich, St. Louis, MO, USA </td><td> 87689 </td><td> N, N, N &#39;, N&#39;-tetrametyletylendiamin </td></tr><tr><td></td><td></td><td></td><td> Utstyr </td></tr><tr><td> Sonikator (3 mm tip) </td&gt; <td> Sonics &amp; Materials, Inc., Newtown, CT, USA </td><td> Vibra-Cell </td><td></td></tr><tr><td> Vannbad </td><td> Thermo Fisher Scientific, Waltham, MA, USA </td><td> Neslab EX-7 </td><td></td></tr><tr><td> Ekstruder </td><td> Nordlige Lipider, Burnaby, BC, Canada </td><td> LIPEX </td><td></td></tr><tr><td> Extruder Filters </td><td> Whatman, Piscataway, NJ, USA </td><td> Nuclepore # 110605 og # 110606 </td><td></td></tr><tr><td> Ekstruder Drain Disc </td><td> Sterlitech Corporation, Kent, WA, USA </td><td> # PETEDD25100 </td><td></td></tr><tr><td> Plastkammer </td><td> US Plastic Corporation, Lima, OH, USA </td><td> # 55288, 1 3/16 &quot;x 1 3/16&quot; x2 7/16 &quot; </td><td></td></tr></tbody></table>

synthesis of phase-shift nanoemulsions with narrow size distributions for acoustic droplet vaporization and bubble-enhanced ultrasound-mediated ablation

Høy intensitet fokusert ultralyd (HIFU) brukes klinisk til termisk ablate svulster. For å forbedre lokal oppvarmning og forbedre termisk ablasjon i tumorer, har lipid-belagt perfluorocarbon dråper blitt utviklet som kan fordampes ved HIFU. Blodkar i mange svulster er unormalt lekk på grunn av sin raske vekst, og nanopartikler er i stand til å trenge gjennom fenestrations og passivt akkumuleres i svulster. Således kan kontrollere størrelsen av dråpene resultere i bedre akkumulering i tumorer. I denne rapporten er utarbeidelse av stabile dråper i en fase-shift nanoemulsion (PSNE) med en smal størrelsesfordeling beskrevet. PSNE ble syntetisert ved sonicating en lipidoppløsningen i nærvær av flytende perfluorkarbonene. En smal størrelsesfordeling ble oppnådd ved å ekstrudere de PSNE flere ganger ved hjelp av filtre med porestørrelser på 100 eller 200 nm. Størrelsesfordelingen ble målt over en 7-dagers periode ved hjelp av dynamisk lysspredning. Polyacrylamide hydrogeler inneholder PSNE var forberedt på in vitro eksperimenter. PSNE dråper i hydrogeler ble fordampet med ultralyd og de resulterende bobler forbedret lokalisert oppvarming. Fordampede PSNE muliggjør raskere oppvarming og reduserer også ultralyd intensitet er nødvendig for termisk ablasjon. Dermed er PSNE forventes å øke termisk ablasjon i svulster, potensielt forbedre terapeutiske utfall av HIFU-medierte termisk ablasjon behandlinger.

Watch this Scientific Journal Video about Synthesis of Phase-shift Nanoemulsions with Narrow Size Distributions for Acoustic Droplet Vaporization and Bubble-enhanced Ultrasound-mediated Ablation at Jo

Synthesis of Phase-shift Nanoemulsions with Narrow Size Distributions for Acoustic Droplet Vaporization and Bubble-enhanced Ultrasound-mediated Ablation

Phase-shift nanoemulsions (PSNE) kan fordampet ved hjelp av høy intensitet fokusert ultralyd for å forbedre lokal oppvarming og bedre termisk ablasjon i svulster. I denne rapporten er utarbeidelse av stabile PSNE med en smal størrelsesfordeling beskrevet. Videre er effekten av fordampede PSNE på ultralyd-mediert ablasjon demonstrert i vev-etterligne fantomer.

Syntese av fase-shift nanoemulsions med smale størrelse Distribusjoner for Acoustic Droplet Fordamping og Bubble-forbedret ultralyd-mediert Ablasjon

High-intensity  focused ultrasound (HIFU) is used clinically to thermally ablate tumors. To  enhance localized heating and improve thermal ablation in ...

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Engineering

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