Name: simultaneous intracellular recording of a lumbar motoneuron and the force produced by its motor unit in the adult mouse in vivo
Uploaded: 2012-12-05T12:00:00
Description: Watch this Scientific Journal Video about Simultaneous Intracellular Recording of a Lumbar Motoneuron and the Force Produced by its Motor Unit in the Adult Mouse In vivo at JoVE.com

यह काम Fondation डालना ला Recherche MEDICALE (एफ आर एम), ALS अनुसंधान (ALS एसोसिएशन), NIH NINDS NS05462 और NS034382 अनुदान, और ANR अनुदान HyperMND के लिए मिल्टन Safenowitz Postdoctoral फैलोशिप से वित्तीय सहायता के लिए संभव धन्यवाद किया गया था.

1. एक कदम पूर्व चतनाशून्य करनेवाली औषधि दवा: संज्ञाहरण के शामिल होने से पहले 10-15 मिनट, atropine (0.20 मिलीग्राम / किग्रा) और (0.05 मिलीग्राम) methylprenidsolone उप cutaneously इंजेक्षन राल निकालना और edema, क्रमशः रोकने के. <p class="jove_tit...

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यहाँ वर्णित तैयारी पहली बार है कि वयस्क माउस, एक काठ motoneuron और पेशी अपने अक्षतंतु द्वारा innervated फाइबर द्वारा उत्पादित बल की माप के साथ - साथ intracellular रिकॉर्डिंग की अनुमति देता है, है. जानवर के छोटे आकार ...

A correction was made to <a href="http://www.jove.com/video/4312">Simultaneous Intracellular Recording of a Lumbar Motoneuron and the Force Produced by its Motor Unit in the Adult Mouse In vivo</a>. There was an error in the name of one author, Marin Manuel. The author's name has been corrected to:
Marin Manuel 
 
instead of: 
 
Manuel Marin

A correction to author list has been made for article <a href="http://www.jove.com/video/4312">Simultaneous Intracellular Recording of a Lumbar Motoneuron and the Force Produced by its Motor Unit in the Adult Mouse In vivo</a>.

Erratum: Simultaneous Intracellular Recording of a Lumbar Motoneuron and the Force Produced by its Motor Unit in the Adult Mouse In Vivo

<table border="1"><tbody><tr><td> अभिकर्मक के नाम </td><td> कंपनी </td><td> सूचीपत्र संख्या </td><td> टिप्पणियां (वैकल्पिक) </td></tr><tr><td> Atropine सल्फेट </td><td> Aguettant </td><td></td><td></td></tr><tr><td> Methylprenidsolone </td><td> फ़िज़र </td><td> Solu - Medrol </td><td></td></tr><tr><td> सोडियम pentobarbitone </td><td> Sanofi-एवेंटिस </td><td> Pentobarbital </td><td></td></tr><tr><td> Ketamine </td><td></td><td></td><td></td></tr><tr><td> Xylazine </td><td></td><td></td><td></td></tr><tr><td> ग्लूकोज </td><td></td><td></td><td></td></tr><tr><td> प्लाज्मा विस्तारक </td><td> रोजर Bellon </td><td> Plasmagel </td><td></td></tr><tr><td> ब्लंट कैंची </td><td> FST </td><td> 14079-10 </td><td></td></tr><tr><td> ब्लंट ठीक कैंची </td><td> FST </td><td> 15025-10 </td><td></td></tr><tr><td> Vannas स्प्रिंग कैंची </td><td> FST </td><td> 15002-08 </td><td></td></tr><tr><td> ठीक संदंश दाँतेदार </td><td> FST </td><td> 11370-32 </td><td></td></tr><tr><td> ठीक संदंश दाँतेदार </td><td> FST </td><td> 11370-31 </td><td></td></tr><tr><td> कनिंघम रीढ़ की हड्डी अडैप्टर </td><td> Stoelting कं </td><td></td><td></td></tr><tr><td> Kwik - कास्ट सीलेंट </td><td> थोक मूल्य सूचकांक </td><td> Kwik - कास्ट # </td><td></td></tr><tr><td> पंखा </td><td> CWE इंक </td><td> SAR-830/AP </td><td></td></tr><tr><td> Capnograph </td><td> CWE इंक </td><td> μcapstar </td><td></td></tr><tr><td> ताप कंबल </td><td> हार्वर्ड उपकरण </td><td> 507221F </td><td></td></tr><tr><td> Intracellular एम्पलीफायर </td><td> Axon instrumeपड़ोसी देशों की यात्रा योजना </td><td> 2B Axoclamp </td><td></td></tr><tr><td> पिपेट डांड़ी </td><td> Sutter उपकरण </td><td> P-97 </td><td></td></tr><tr><td> KCl </td><td> सिग्मा Aldrich </td><td> P9333-500G </td><td></td></tr></tbody></table>

simultaneous intracellular recording of a lumbar motoneuron and the force produced by its motor unit in the adult mouse in vivo

The spinal motoneuron has long been a good model system for studying neural function because it is a neuron of the central nervous system with the unique properties of (1) having readily identifiable targets (the muscle fibers) and therefore having a very well-known function (to control muscle contraction); (2) being the convergent target of many spinal and descending networks, hence the name of "final common pathway"; and (3) having a large soma which makes it possible to penetrate them with sharp intracellular electrodes. Furthermore, when studied in vivo, it is possible to record simultaneously the electrical activity of the motoneurons and the force developed by their muscle targets. Performing intracellular recordings of motoneurons in vivo therefore put the experimentalist in the unique position of being able to study, at the same time, all the compartments of the "motor unit" (the name given to the motoneuron, its axon, and the muscle fibers it innervates1): the inputs impinging on the motoneuron, the electrophysiological properties of the motoneuron, and the impact of these properties on the physiological function of the motoneurons, i.e. the force produced by its motor unit. However, this approach is very challenging because the preparation cannot be paralyzed and thus the mechanical stability for the intracellular recording is reduced. Thus, this kind of experiments has only been achieved in cats and in rats. However, the study of spinal motor systems could make a formidable leap if it was possible to perform similar experiments in normal and genetically modified mice.
For technical reasons, the study of the spinal networks in mice has mostly been limited to neonatal in vitro preparations, where the motoneurons and the spinal networks are immature, the motoneurons are separated from their targets, and when studied in slices, the motoneurons are separated from most of their inputs. Until recently, only a few groups had managed to perform intracellular recordings of motoneurons in vivo2-4 , including our team who published a new preparation which allowed us to obtain very stable recordings of motoneurons in vivo in adult mice5,6. However, these recordings were obtained in paralyzed animals, i.e. without the possibility to record the force output of these motoneurons. Here we present an extension of this original preparation in which we were able to obtain simultaneous recordings of the electrophysiological properties of the motoneurons and of the force developed by their motor unit. This is an important achievement, as it allows us to identify the different types of motoneurons based on their force profile, and thereby revealing their function. Coupled with genetic models disturbing spinal segmental circuitry7-9, or reproducting human disease10,11, we expect this technique to be an essential tool for the study of spinal motor system.

चित्रा 1 से पता चलता है कि किस तरह प्रवेश के बाद Triceps Surae समूह से एक motoneuron की पहचान करने के लिए. कम उत्तेजना की तीव्रता में, केवल एक monosynaptic EPSP (चित्रा 1 ए) देखा जा सकता है. उच्च तीव्रता EPSP काफी बड़े के लिए ए...

Watch this Scientific Journal Video about Simultaneous Intracellular Recording of a Lumbar Motoneuron and the Force Produced by its Motor Unit in the Adult Mouse In vivo at JoVE.com

Simultaneous Intracellular Recording of a Lumbar Motoneuron and the Force Produced by its Motor Unit in the Adult Mouse In vivo

इस नई विधि में एक एकल वयस्क माउस motoneuron और अपनी मांसपेशी फाइबर द्वारा उत्पादित बल की माप के साथ - साथ intracellular रिकॉर्डिंग परमिट. सामान्य और आनुवंशिक रूप से संशोधित पशुओं में मोटर इकाइयों के विद्युत और यांत्रिक गुणों की संयुक्त जांच neuromuscular प्रणाली के अध्ययन के लिए एक सफलता है.

एक काठ Motoneuron और फोर्स वयस्क चूहे में अपनी मोटर यूनिट द्वारा उत्पादित के साथ - साथ Intracellular रिकॉर्डिंग<em&gt; Vivo में</em

The  spinal motoneuron has long been a good model system for studying neural  function because it is a neuron of the central nervous system with the ...

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Simultaneous Intracellular Recording of a Lumbar Motoneuron and the Force Produced by its Motor Unit in the Adult Mouse In vivo

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