Name: bioluminescent bacterial imaging in vivo
Uploaded: 2012-11-04T23:00:00
Description: Watch this Scientific Journal Video about Bioluminescent Bacterial Imaging In Vivo at JoVE.com

Die Autoren möchten die Unterstützung in bezug auf dieses Manuskript von der Europäischen Kommission Siebten Rahmenprogramms (PIOF-GA-2009 bis 255.466) und dem Irish Health Research Board (HRA_POR/2010/138) quittieren. Lux-markierten E. coli war eine Art Geschenk von Dr. Cormac Gahan, University College Cork.

Ein. Tumorinduktion <ol><li> Für die Routine Tumorinduktion wurde die minimale tumorigenen Dosis von Zellen in 200 ul serumfreiem Kulturmedium suspendiert subkutan (sc) in die Flanke frei von Infektionen 6-8 Wochen alte weibliche Balb / C oder athymischen MF1-nu/nu Mäusen injiziert n = 6 (Harlan, Oxfordshire, UK) (1 x 10 6 4T1-Zellen) mit einer 21-Gauge Injektionsnadel. Die Lebensfähigkeit der Zellen zur Inokulation verwendet wurde, war größer als 95%, wie durch visuelle Zählung mit einem Hämozytomet...

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Im Rahmen der Gentherapie hat sich die Verwendung von biologischen Agenzien zur Abgabe von therapeutischen Genen für Patienten sehr vielversprechend 3-5 gezeigt. Wie Viren erlauben die angeborenen biologischen Eigenschaften von Bakterien effizienten DNA-Abgabe an Zellen oder Gewebe, insbesondere im Zusammenhang mit Krebserkrankungen. Es hat sich gezeigt, daß Bakterien in der Lage sind natürlich Homing auf Tumoren, wenn systemisch verabreicht, was zu hohen Replikation lokal entweder außerhalb (nicht-invasi...

<table border="1"><tbody><tr><td> Name des Reagenzes </td><td> Firma </td><td> Katalog-Nummer </td><td> Kommentare </td></tr><tr><td> 4T1-Zelllinie </td><td> ATCC </td><td> CRL-2539 </td><td> Syngenen Brustkrebs-Modell aus einer spontan entstehenden BALB / c Brusttumor abgeleitet </td></tr><tr><td> DMEM </td><td> Sigma-Aldrich </td><td> D6429 </td><td> Dulbecco modifiziertem Eagle-Medium </td></tr><tr><td> PBS </td><td> Sigma-Aldrich </td><td> D8537 </td><td> Phosphate Buffered Saline </td></tr><tr><td> Xenogen IVIS </td><td> Caliper Life Sciences </td><td></td><td> IVIS 100 für 2D-Bildgebung; IVIS Spectrum für 3D. </td></tr><tr><td> Luria Broth Miller (LB) </td><td> Sigma-Aldrich </td><td> L2542 </td><td> Wachstumsmedium für E. coli </td></tr&gt; <tr><td> Erythromycin </td><td> Sigma-Aldrich </td><td> E5389 </td><td> Antibiotikum </td></tr><tr><td> Streptomycin </td><td> Sigma-Aldrich </td><td> S9137 </td><td> Antibiotikum </td></tr><tr><td> MF1nu/nu Mäusen </td><td> Harlan (UK) </td><td> 069 (nu) / 070 (nu / +) </td><td> Hsd: athymischer-Foxn1nu </td></tr><tr><td> Balb / c-Mäusen </td><td> Harlan (UK) </td><td> 066 </td><td> Haplotyp: H-2 d </td></tr><tr><td> Gavage Nadel </td><td> Vet-Tech-Solutions (UK) </td><td> DE009 </td><td> 22G x 38mm gerade Magensonde Nadel </td></tr><tr><td> Spritze für iv Injektion </td><td> BD Biosciences </td><td> 309.309 bis 1 ml </td><td> Insulinspritze mit 28 G x ½ Zoll mikro-feinen IV Nadel. </td></tr><tr><td> Spritze für Tumorinokulation </td><td> Braun </td><td> 9161376V </td><td> Omnifix 26 G x &amp;frac12; Zoll-Nadel </td></tr></tbody></table>

bioluminescent bacterial imaging in vivo

Dieses Video beschreibt den Einsatz des ganzen Körpers bioluminesce Imaging (BLI) für die Untersuchung von bakteriellen Handel mit lebenden Mäusen, mit einem Schwerpunkt auf der Verwendung von Bakterien in der Gen-und Zelltherapie bei Krebs. Bakterien stellen eine attraktive Klasse von Vektor für die Krebstherapie, die über eine natürliche Fähigkeit, vorzugsweise wachsen innerhalb Tumoren nach systemischer Verabreichung. Bakterien entwickelt, um die lux Genkassette Erlaubnis BLI Nachweis der Bakterien und gleichzeitig Tumorstellen auszudrücken. Die Lage und Höhe der Bakterien innerhalb Tumoren im Laufe der Zeit leicht geprüft werden kann, visualisiert in zwei oder drei Dimensionen. Das Verfahren ist für ein breites Spektrum von Bakterien-Spezies und Tumorxenotransplantat Typen. Dieser Artikel beschreibt das Protokoll für die Analyse der Biolumineszenz Bakterien im subkutanen Tumor tragenden Mäusen. Visualisierung von symbiotischer Bakterien im Magen-Darm-Trakt (GIT) von BLI wird ebenfalls beschrieben. Diese leistungsstarke und billig, Bildgebung in Echtzeit Strategie Vertreterts eine ideale Methode zur Untersuchung von Bakterien in vivo im Kontext der Krebsforschung, insbesondere Gentherapie, und Infektionskrankheiten. Dieses Video beschreibt das Verfahren für die Untersuchung lux-markierten E. coli in lebenden Mäusen, zeigt die räumliche und zeitliche Auslesen erreichbaren Nutzung BLI mit dem IVIS System.

Watch this Scientific Journal Video about Bioluminescent Bacterial Imaging In Vivo at JoVE.com

Bioluminescent Bacterial Imaging In Vivo

Dieser Artikel beschreibt die Verwaltung von<em&gt; Lux-getaggten</em&gt; Bakterien an Mäusen und anschließende<em&gt; In vivo</em&gt; Analyse mit IVIS Biolumineszenz-Bildgebung.

Bioluminescent Bakterielle Imaging<em&gt; In Vivo</em

This video describes the use of whole body bioluminesce imaging (BLI) for the study of bacterial trafficking in live mice, with an emphasis on the use of ...

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Bioluminescent Bacterial Imaging In Vivo

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Invasion and colonization of host cells by bacterial pathogens depend on the activity of a large number of prokaryotic proteins, defined as virulence ...

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In order to cause endovascular infections and infective endocarditis, bacteria need to be able to adhere to the vessel wall while being exposed to the ...