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DOI :
10.3791/4321-v
•
16:59 min
October 10th, 2012
Chapters
0:05
Title
2:00
Helper-dependent Adenoviral Vector Production and Amplification
5:02
Large Scale HDAd Production
7:13
Vector Purification
11:19
Treatment of Diabetic Mice by HDAd-Ngn3 and -Btc
13:08
Evidence for Neo-islet Formation in Liver: Representative Results
16:14
Conclusion
我们描述了肝形成新胰岛STZ(链脲佐菌素)诱导的糖尿病小鼠的转基因Neurogenin3(Ngn3)和β细胞素(BTC)的使用依赖辅助腺病毒载体(HDAd)和反转高血糖。我们的方法的优点帮手依赖型腺病毒载体,其高度有效的体内转导和持久的基因表达。
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