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DOI :
10.3791/4333-v
•
13:18 min
December 29th, 2012
Chapters
0:05
Title
1:36
Generation of Artificial Antigen Presenting Cells (aAPC)
3:39
Isolation of CD161+CD3+ Cells
7:22
aAPC-mediated NKT Cell Expansion
8:16
Functional Test: aAPC-mediated Stimulation of NKT Cells
8:47
Results: aAPC-mediated Activation and Expansion of NKT Cells
12:48
Conclusion
ここでは、人工抗原提示細胞(AAPC)を使用してバルクT細胞集団から、ヒトNKT細胞を活性化し、拡大するための方法を説明します。 CD1dのベースAAPCの使用は、機能的なNKT細胞の高い数値を生成するための標準化された方法を提供します。
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